Data extracted from this reference:
General Stability
2.7.1.77
dialysis first against EDTA or oxime and then against water produces an inhibition which results in the recovery of 36% of the initial phosphotransferase activity and 65% of the initial phosphatase activity
Daucus carota
2.7.1.77
no loss of activity after dialysis overnight against distilled water
Daucus carota
Inhibitors
2.7.1.77
Ca2+
Daucus carota
2.7.1.77
cysteine
2.5 mM, depresses both transferase and phosphatase activity by about 25%
Daucus carota
2.7.1.77
EDTA
0.625 mM, inhibition is reversed by 5 mM MgCl2
Daucus carota
2.7.1.77
Fe2+
Daucus carota
2.7.1.77
Fe3+
Daucus carota
2.7.1.77
glutathione
2.5 mM, depresses both transferase and phosphatase activity by about 25%
Daucus carota
2.7.1.77
Mn2+
Daucus carota
2.7.1.77
Pb2+
Daucus carota
Metals/Ions
2.7.1.77
Co2+
enhances phosphatase activity considerably, but transferase activity to a small extent
Daucus carota
2.7.1.77
Cu2+
enhances phosphatase activity considerably, but transferase activity to a small extent
Daucus carota
2.7.1.77
Mg2+
enhances phosphatase activity considerably, but transferase activity to a small extent
Daucus carota
Molecular Weight [Da]
2.7.1.77
additional information
the particle weight of the enzyme as determined by gel filtration varies with pH. At pH 5.0 one species of 45000 Da is detected, at pH 9.5 two species of 44000 Da and 63000 Da are observed
Daucus carota
Organism
Purification (Commentary)
Source Tissue
2.7.1.77
root
Daucus carota
Specific Activity [micromol/min/mg]
2.7.1.77
additional information
Daucus carota
Storage Stability
2.7.1.77
-20°C, stable for at least 1 months
Daucus carota
2.7.1.77
4°C, 1 week, 80% of the transferase activity is retained, no loss of hydrolase activity
Daucus carota
Substrates and Products (Substrate)
2.7.1.77
(2',3')-uridylic acid + uridine
about 10% of the activity with phenyl phosphate
641990
Daucus carota
uridine + UMP
?
2.7.1.77
3'-AMP + uridine
70% of the activity with phenyl phosphate
641990
Daucus carota
adenosine + UMP
?
2.7.1.77
5'-adenylic acid + uridine
70% of the activity with phenyl phosphate
641990
Daucus carota
adenosine + UMP
?
2.7.1.77
beta-glycerophosphate + uridine
about 15% of the activity with phenyl phosphate
641990
Daucus carota
glycerol + UMP
?
2.7.1.77
D-ribose 5-phosphate + uridine
about 30% of the activity with phenyl phosphate
641990
Daucus carota
D-ribose + UMP
?
2.7.1.77
additional information
the enzyme also exhibits phosphatase activity
641990
Daucus carota
?
2.7.1.77
additional information
protein carries both transfer and hydrolytic functions
641990
Daucus carota
?
2.7.1.77
phenyl phosphate + adenosine
641990
Daucus carota
AMP + phenol
?
2.7.1.77
phenyl phosphate + cytidine
641990
Daucus carota
CMP + phenol
?
2.7.1.77
phenyl phosphate + deoxyadenosine
641990
Daucus carota
dAMP + phenol
?
2.7.1.77
phenyl phosphate + deoxycytidine
641990
Daucus carota
dCMP + phenol
?
2.7.1.77
phenyl phosphate + deoxyguanosine
641990
Daucus carota
dGMP + phenol
?
2.7.1.77
phenyl phosphate + deoxyuridine
641990
Daucus carota
dUMP + phenol
?
2.7.1.77
phenyl phosphate + guanosine
641990
Daucus carota
GMP + phenol
?
2.7.1.77
phenyl phosphate + thymidine
641990
Daucus carota
TMP + phenol
?
2.7.1.77
phenyl phosphate + uridine
641990
Daucus carota
5'-UMP + phenol
?
pH Optimum
2.7.1.77
5
reaction with phenylphosphate and uridine or deoxycytidine
Daucus carota
pH Range
2.7.1.77
4
7
pH 4.0: about 25% of maximal activity in the reaction with phenyl phosphate and uridine, about 40% of maximal activity with phenyl phosphate and deoxycytidine , pH 7.0: about 20% of maximal activity in the reaction with phenyl phosphate and uridine, about 50% of maximal activity with phenyl phosphate and deoxycytidine
Daucus carota
General Stability (protein specific)
2.7.1.77
dialysis first against EDTA or oxime and then against water produces an inhibition which results in the recovery of 36% of the initial phosphotransferase activity and 65% of the initial phosphatase activity
Daucus carota
2.7.1.77
no loss of activity after dialysis overnight against distilled water
Daucus carota
Inhibitors (protein specific)
2.7.1.77
Ca2+
Daucus carota
2.7.1.77
cysteine
2.5 mM, depresses both transferase and phosphatase activity by about 25%
Daucus carota
2.7.1.77
EDTA
0.625 mM, inhibition is reversed by 5 mM MgCl2
Daucus carota
2.7.1.77
Fe2+
Daucus carota
2.7.1.77
Fe3+
Daucus carota
2.7.1.77
glutathione
2.5 mM, depresses both transferase and phosphatase activity by about 25%
Daucus carota
2.7.1.77
Mn2+
Daucus carota
2.7.1.77
Pb2+
Daucus carota
Metals/Ions (protein specific)
2.7.1.77
Co2+
enhances phosphatase activity considerably, but transferase activity to a small extent
Daucus carota
2.7.1.77
Cu2+
enhances phosphatase activity considerably, but transferase activity to a small extent
Daucus carota
2.7.1.77
Mg2+
enhances phosphatase activity considerably, but transferase activity to a small extent
Daucus carota
Molecular Weight [Da] (protein specific)
2.7.1.77
additional information
the particle weight of the enzyme as determined by gel filtration varies with pH. At pH 5.0 one species of 45000 Da is detected, at pH 9.5 two species of 44000 Da and 63000 Da are observed
Daucus carota
Purification (Commentary) (protein specific)
Source Tissue (protein specific)
2.7.1.77
root
Daucus carota
Specific Activity [micromol/min/mg] (protein specific)
2.7.1.77
additional information
Daucus carota
Storage Stability (protein specific)
2.7.1.77
-20°C, stable for at least 1 months
Daucus carota
2.7.1.77
4°C, 1 week, 80% of the transferase activity is retained, no loss of hydrolase activity
Daucus carota
Substrates and Products (Substrate) (protein specific)
2.7.1.77
(2',3')-uridylic acid + uridine
about 10% of the activity with phenyl phosphate
641990
Daucus carota
uridine + UMP
?
2.7.1.77
3'-AMP + uridine
70% of the activity with phenyl phosphate
641990
Daucus carota
adenosine + UMP
?
2.7.1.77
5'-adenylic acid + uridine
70% of the activity with phenyl phosphate
641990
Daucus carota
adenosine + UMP
?
2.7.1.77
beta-glycerophosphate + uridine
about 15% of the activity with phenyl phosphate
641990
Daucus carota
glycerol + UMP
?
2.7.1.77
D-ribose 5-phosphate + uridine
about 30% of the activity with phenyl phosphate
641990
Daucus carota
D-ribose + UMP
?
2.7.1.77
additional information
the enzyme also exhibits phosphatase activity
641990
Daucus carota
?
2.7.1.77
additional information
protein carries both transfer and hydrolytic functions
641990
Daucus carota
?
2.7.1.77
phenyl phosphate + adenosine
641990
Daucus carota
AMP + phenol
?
2.7.1.77
phenyl phosphate + cytidine
641990
Daucus carota
CMP + phenol
?
2.7.1.77
phenyl phosphate + deoxyadenosine
641990
Daucus carota
dAMP + phenol
?
2.7.1.77
phenyl phosphate + deoxycytidine
641990
Daucus carota
dCMP + phenol
?
2.7.1.77
phenyl phosphate + deoxyguanosine
641990
Daucus carota
dGMP + phenol
?
2.7.1.77
phenyl phosphate + deoxyuridine
641990
Daucus carota
dUMP + phenol
?
2.7.1.77
phenyl phosphate + guanosine
641990
Daucus carota
GMP + phenol
?
2.7.1.77
phenyl phosphate + thymidine
641990
Daucus carota
TMP + phenol
?
2.7.1.77
phenyl phosphate + uridine
641990
Daucus carota
5'-UMP + phenol
?
pH Optimum (protein specific)
2.7.1.77
5
reaction with phenylphosphate and uridine or deoxycytidine
Daucus carota
pH Range (protein specific)
2.7.1.77
4
7
pH 4.0: about 25% of maximal activity in the reaction with phenyl phosphate and uridine, about 40% of maximal activity with phenyl phosphate and deoxycytidine , pH 7.0: about 20% of maximal activity in the reaction with phenyl phosphate and uridine, about 50% of maximal activity with phenyl phosphate and deoxycytidine
Daucus carota