show Purification and properties of a nucleoside phosphotransferase from carrot
Brunngraber, E.F.; Chargaff, E.; J. Biol. Chem. 242, 4834-4840 (1967)
Data extracted from this reference:
General Stability
EC Number
General Stability
Organism
2.7.1.77
dialysis first against EDTA or oxime and then against water produces an inhibition which results in the recovery of 36% of the initial phosphotransferase activity and 65% of the initial phosphatase activity
Daucus carota
2.7.1.77
no loss of activity after dialysis overnight against distilled water
Daucus carota
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.77
Ca2+
-
Daucus carota
2.7.1.77
cysteine
2.5 mM, depresses both transferase and phosphatase activity by about 25%
Daucus carota
2.7.1.77
EDTA
0.625 mM, inhibition is reversed by 5 mM MgCl2
Daucus carota
2.7.1.77
Fe2+
-
Daucus carota
2.7.1.77
Fe3+
-
Daucus carota
2.7.1.77
glutathione
2.5 mM, depresses both transferase and phosphatase activity by about 25%
Daucus carota
2.7.1.77
Mn2+
-
Daucus carota
2.7.1.77
Pb2+
-
Daucus carota
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.77
Co2+
enhances phosphatase activity considerably, but transferase activity to a small extent
Daucus carota
2.7.1.77
Cu2+
enhances phosphatase activity considerably, but transferase activity to a small extent
Daucus carota
2.7.1.77
Mg2+
enhances phosphatase activity considerably, but transferase activity to a small extent
Daucus carota
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.77
additional information
-
the particle weight of the enzyme as determined by gel filtration varies with pH. At pH 5.0 one species of 45000 Da is detected, at pH 9.5 two species of 44000 Da and 63000 Da are observed
Daucus carota
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.77
Daucus carota
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.77
-
Daucus carota
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.77
root
-
Daucus carota
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.77
additional information
-
-
Daucus carota
Storage Stability
EC Number
Storage Stability
Organism
2.7.1.77
-20°C, stable for at least 1 months
Daucus carota
2.7.1.77
4°C, 1 week, 80% of the transferase activity is retained, no loss of hydrolase activity
Daucus carota
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.77
(2',3')-uridylic acid + uridine
about 10% of the activity with phenyl phosphate
641990
Daucus carota
uridine + UMP
-
-
-
?
2.7.1.77
3'-AMP + uridine
70% of the activity with phenyl phosphate
641990
Daucus carota
adenosine + UMP
-
-
-
?
2.7.1.77
5'-adenylic acid + uridine
70% of the activity with phenyl phosphate
641990
Daucus carota
adenosine + UMP
-
-
-
?
2.7.1.77
beta-glycerophosphate + uridine
about 15% of the activity with phenyl phosphate
641990
Daucus carota
glycerol + UMP
-
-
-
?
2.7.1.77
D-ribose 5-phosphate + uridine
about 30% of the activity with phenyl phosphate
641990
Daucus carota
D-ribose + UMP
-
-
-
?
2.7.1.77
additional information
the enzyme also exhibits phosphatase activity
641990
Daucus carota
?
-
-
-
-
2.7.1.77
additional information
protein carries both transfer and hydrolytic functions
641990
Daucus carota
?
-
-
-
-
2.7.1.77
phenyl phosphate + adenosine
-
641990
Daucus carota
AMP + phenol
-
-
-
?
2.7.1.77
phenyl phosphate + cytidine
-
641990
Daucus carota
CMP + phenol
-
-
-
?
2.7.1.77
phenyl phosphate + deoxyadenosine
-
641990
Daucus carota
dAMP + phenol
-
-
-
?
2.7.1.77
phenyl phosphate + deoxycytidine
-
641990
Daucus carota
dCMP + phenol
-
-
-
?
2.7.1.77
phenyl phosphate + deoxyguanosine
-
641990
Daucus carota
dGMP + phenol
-
-
-
?
2.7.1.77
phenyl phosphate + deoxyuridine
-
641990
Daucus carota
dUMP + phenol
-
-
-
?
2.7.1.77
phenyl phosphate + guanosine
-
641990
Daucus carota
GMP + phenol
-
-
-
?
2.7.1.77
phenyl phosphate + thymidine
-
641990
Daucus carota
TMP + phenol
-
-
-
?
2.7.1.77
phenyl phosphate + uridine
-
641990
Daucus carota
5'-UMP + phenol
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.77
5
-
reaction with phenylphosphate and uridine or deoxycytidine
Daucus carota
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.77
4
7
pH 4.0: about 25% of maximal activity in the reaction with phenyl phosphate and uridine, about 40% of maximal activity with phenyl phosphate and deoxycytidine , pH 7.0: about 20% of maximal activity in the reaction with phenyl phosphate and uridine, about 50% of maximal activity with phenyl phosphate and deoxycytidine
Daucus carota
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.1.77
dialysis first against EDTA or oxime and then against water produces an inhibition which results in the recovery of 36% of the initial phosphotransferase activity and 65% of the initial phosphatase activity
Daucus carota
2.7.1.77
no loss of activity after dialysis overnight against distilled water
Daucus carota
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.77
Ca2+
-
Daucus carota
2.7.1.77
cysteine
2.5 mM, depresses both transferase and phosphatase activity by about 25%
Daucus carota
2.7.1.77
EDTA
0.625 mM, inhibition is reversed by 5 mM MgCl2
Daucus carota
2.7.1.77
Fe2+
-
Daucus carota
2.7.1.77
Fe3+
-
Daucus carota
2.7.1.77
glutathione
2.5 mM, depresses both transferase and phosphatase activity by about 25%
Daucus carota
2.7.1.77
Mn2+
-
Daucus carota
2.7.1.77
Pb2+
-
Daucus carota
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.77
Co2+
enhances phosphatase activity considerably, but transferase activity to a small extent
Daucus carota
2.7.1.77
Cu2+
enhances phosphatase activity considerably, but transferase activity to a small extent
Daucus carota
2.7.1.77
Mg2+
enhances phosphatase activity considerably, but transferase activity to a small extent
Daucus carota
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.77
additional information
-
the particle weight of the enzyme as determined by gel filtration varies with pH. At pH 5.0 one species of 45000 Da is detected, at pH 9.5 two species of 44000 Da and 63000 Da are observed
Daucus carota
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.77
-
Daucus carota
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.77
root
-
Daucus carota
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.77
additional information
-
-
Daucus carota
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.1.77
-20°C, stable for at least 1 months
Daucus carota
2.7.1.77
4°C, 1 week, 80% of the transferase activity is retained, no loss of hydrolase activity
Daucus carota
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.77
(2',3')-uridylic acid + uridine
about 10% of the activity with phenyl phosphate
641990
Daucus carota
uridine + UMP
-
-
-
?
2.7.1.77
3'-AMP + uridine
70% of the activity with phenyl phosphate
641990
Daucus carota
adenosine + UMP
-
-
-
?
2.7.1.77
5'-adenylic acid + uridine
70% of the activity with phenyl phosphate
641990
Daucus carota
adenosine + UMP
-
-
-
?
2.7.1.77
beta-glycerophosphate + uridine
about 15% of the activity with phenyl phosphate
641990
Daucus carota
glycerol + UMP
-
-
-
?
2.7.1.77
D-ribose 5-phosphate + uridine
about 30% of the activity with phenyl phosphate
641990
Daucus carota
D-ribose + UMP
-
-
-
?
2.7.1.77
additional information
the enzyme also exhibits phosphatase activity
641990
Daucus carota
?
-
-
-
-
2.7.1.77
additional information
protein carries both transfer and hydrolytic functions
641990
Daucus carota
?
-
-
-
-
2.7.1.77
phenyl phosphate + adenosine
-
641990
Daucus carota
AMP + phenol
-
-
-
?
2.7.1.77
phenyl phosphate + cytidine
-
641990
Daucus carota
CMP + phenol
-
-
-
?
2.7.1.77
phenyl phosphate + deoxyadenosine
-
641990
Daucus carota
dAMP + phenol
-
-
-
?
2.7.1.77
phenyl phosphate + deoxycytidine
-
641990
Daucus carota
dCMP + phenol
-
-
-
?
2.7.1.77
phenyl phosphate + deoxyguanosine
-
641990
Daucus carota
dGMP + phenol
-
-
-
?
2.7.1.77
phenyl phosphate + deoxyuridine
-
641990
Daucus carota
dUMP + phenol
-
-
-
?
2.7.1.77
phenyl phosphate + guanosine
-
641990
Daucus carota
GMP + phenol
-
-
-
?
2.7.1.77
phenyl phosphate + thymidine
-
641990
Daucus carota
TMP + phenol
-
-
-
?
2.7.1.77
phenyl phosphate + uridine
-
641990
Daucus carota
5'-UMP + phenol
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.77
5
-
reaction with phenylphosphate and uridine or deoxycytidine
Daucus carota
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.77
4
7
pH 4.0: about 25% of maximal activity in the reaction with phenyl phosphate and uridine, about 40% of maximal activity with phenyl phosphate and deoxycytidine , pH 7.0: about 20% of maximal activity in the reaction with phenyl phosphate and uridine, about 50% of maximal activity with phenyl phosphate and deoxycytidine
Daucus carota