EC Number | General Stability | Organism |
---|---|---|
2.7.1.77 | dialysis first against EDTA or oxime and then against water produces an inhibition which results in the recovery of 36% of the initial phosphotransferase activity and 65% of the initial phosphatase activity | Daucus carota |
2.7.1.77 | no loss of activity after dialysis overnight against distilled water | Daucus carota |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.77 | Ca2+ | - |
Daucus carota | |
2.7.1.77 | cysteine | 2.5 mM, depresses both transferase and phosphatase activity by about 25% | Daucus carota | |
2.7.1.77 | EDTA | 0.625 mM, inhibition is reversed by 5 mM MgCl2 | Daucus carota | |
2.7.1.77 | Fe2+ | - |
Daucus carota | |
2.7.1.77 | Fe3+ | - |
Daucus carota | |
2.7.1.77 | glutathione | 2.5 mM, depresses both transferase and phosphatase activity by about 25% | Daucus carota | |
2.7.1.77 | Mn2+ | - |
Daucus carota | |
2.7.1.77 | Pb2+ | - |
Daucus carota |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.77 | Co2+ | enhances phosphatase activity considerably, but transferase activity to a small extent | Daucus carota | |
2.7.1.77 | Cu2+ | enhances phosphatase activity considerably, but transferase activity to a small extent | Daucus carota | |
2.7.1.77 | Mg2+ | enhances phosphatase activity considerably, but transferase activity to a small extent | Daucus carota |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.77 | additional information | - |
the particle weight of the enzyme as determined by gel filtration varies with pH. At pH 5.0 one species of 45000 Da is detected, at pH 9.5 two species of 44000 Da and 63000 Da are observed | Daucus carota |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.77 | Daucus carota | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.77 | - |
Daucus carota |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.1.77 | root | - |
Daucus carota | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.77 | additional information | - |
- |
Daucus carota |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.1.77 | -20°C, stable for at least 1 months | Daucus carota |
2.7.1.77 | 4°C, 1 week, 80% of the transferase activity is retained, no loss of hydrolase activity | Daucus carota |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.77 | (2',3')-uridylic acid + uridine | about 10% of the activity with phenyl phosphate | Daucus carota | uridine + UMP | - |
? | |
2.7.1.77 | 3'-AMP + uridine | 70% of the activity with phenyl phosphate | Daucus carota | adenosine + UMP | - |
? | |
2.7.1.77 | 5'-adenylic acid + uridine | 70% of the activity with phenyl phosphate | Daucus carota | adenosine + UMP | - |
? | |
2.7.1.77 | beta-glycerophosphate + uridine | about 15% of the activity with phenyl phosphate | Daucus carota | glycerol + UMP | - |
? | |
2.7.1.77 | D-ribose 5-phosphate + uridine | about 30% of the activity with phenyl phosphate | Daucus carota | D-ribose + UMP | - |
? | |
2.7.1.77 | additional information | the enzyme also exhibits phosphatase activity | Daucus carota | ? | - |
? | |
2.7.1.77 | additional information | protein carries both transfer and hydrolytic functions | Daucus carota | ? | - |
? | |
2.7.1.77 | phenyl phosphate + adenosine | - |
Daucus carota | AMP + phenol | - |
? | |
2.7.1.77 | phenyl phosphate + cytidine | - |
Daucus carota | CMP + phenol | - |
? | |
2.7.1.77 | phenyl phosphate + deoxyadenosine | - |
Daucus carota | dAMP + phenol | - |
? | |
2.7.1.77 | phenyl phosphate + deoxycytidine | - |
Daucus carota | dCMP + phenol | - |
? | |
2.7.1.77 | phenyl phosphate + deoxyguanosine | - |
Daucus carota | dGMP + phenol | - |
? | |
2.7.1.77 | phenyl phosphate + deoxyuridine | - |
Daucus carota | dUMP + phenol | - |
? | |
2.7.1.77 | phenyl phosphate + guanosine | - |
Daucus carota | GMP + phenol | - |
? | |
2.7.1.77 | phenyl phosphate + thymidine | - |
Daucus carota | TMP + phenol | - |
? | |
2.7.1.77 | phenyl phosphate + uridine | - |
Daucus carota | 5'-UMP + phenol | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.77 | 5 | - |
reaction with phenylphosphate and uridine or deoxycytidine | Daucus carota |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.77 | 4 | 7 | pH 4.0: about 25% of maximal activity in the reaction with phenyl phosphate and uridine, about 40% of maximal activity with phenyl phosphate and deoxycytidine , pH 7.0: about 20% of maximal activity in the reaction with phenyl phosphate and uridine, about 50% of maximal activity with phenyl phosphate and deoxycytidine | Daucus carota |