BRENDA - Enzyme Database show

Mammalian deoxynucleoside kinases. II. Deoxyadenosine kinase: purification and properties

Krygier, V.; Momparler, R.L.; J. Biol. Chem. 246, 2745-2751 (1971)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.76
Hg2+
0.01 mM, 25% inhibition, 0.1 mM, almost complete inhibition
Bos taurus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.76
Ca2+
activation, can replace Mg2+
Bos taurus
2.7.1.76
Mg2+
required for activity
Bos taurus
2.7.1.76
Mn2+
activation, can replace Mg2+
Bos taurus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.76
63000
-
gel filtration
Bos taurus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.76
Bos taurus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.76
streptomycin sulfate, protamine sulfate, ammonium sulfate, Sephadex G-150
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.76
thymus
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.76
0.073
-
-
Bos taurus
Storage Stability
EC Number
Storage Stability
Organism
2.7.1.76
-20°C, 60% glycerol, at least 3 months, slight loss of activity
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.76
ATP + cytidine
approx. 18% of activity with deoxyadenosine
641981
Bos taurus
ADP + CMP
-
641981
Bos taurus
?
2.7.1.76
ATP + deoxyadenosine
-
641981
Bos taurus
ADP + dAMP
-
641981
Bos taurus
?
2.7.1.76
ATP + deoxycytidine
approx. 12% of activity with deoxyadenosine
641981
Bos taurus
ADP + dCMP
-
641981
Bos taurus
?
2.7.1.76
ATP + deoxyguanosine
-
641981
Bos taurus
ADP + dGMP
-
641981
Bos taurus
?
2.7.1.76
dTTP + deoxyadenosine
75% of activity with ATP
641981
Bos taurus
dTDP + dAMP
-
641981
Bos taurus
?
2.7.1.76
GTP + deoxyadenosine
-
641981
Bos taurus
GDP + dAMP
-
641981
Bos taurus
?
2.7.1.76
UTP + deoxyadenosine
90% of activity with ATP
641981
Bos taurus
UDP + dAMP
-
641981
Bos taurus
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.76
37
-
assay at
Bos taurus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.76
7.5
-
broad optimum, 10% difference in activity between pH 6.5 and pH 8.5
Bos taurus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.76
5.7
8.8
approx. half-maximal activity at pH 5.7, approx. 80% of maximal activity at pH 8.8
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.76
Hg2+
0.01 mM, 25% inhibition, 0.1 mM, almost complete inhibition
Bos taurus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.76
Ca2+
activation, can replace Mg2+
Bos taurus
2.7.1.76
Mg2+
required for activity
Bos taurus
2.7.1.76
Mn2+
activation, can replace Mg2+
Bos taurus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.76
63000
-
gel filtration
Bos taurus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.76
streptomycin sulfate, protamine sulfate, ammonium sulfate, Sephadex G-150
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.76
thymus
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.76
0.073
-
-
Bos taurus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.1.76
-20°C, 60% glycerol, at least 3 months, slight loss of activity
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.76
ATP + cytidine
approx. 18% of activity with deoxyadenosine
641981
Bos taurus
ADP + CMP
-
641981
Bos taurus
?
2.7.1.76
ATP + deoxyadenosine
-
641981
Bos taurus
ADP + dAMP
-
641981
Bos taurus
?
2.7.1.76
ATP + deoxycytidine
approx. 12% of activity with deoxyadenosine
641981
Bos taurus
ADP + dCMP
-
641981
Bos taurus
?
2.7.1.76
ATP + deoxyguanosine
-
641981
Bos taurus
ADP + dGMP
-
641981
Bos taurus
?
2.7.1.76
dTTP + deoxyadenosine
75% of activity with ATP
641981
Bos taurus
dTDP + dAMP
-
641981
Bos taurus
?
2.7.1.76
GTP + deoxyadenosine
-
641981
Bos taurus
GDP + dAMP
-
641981
Bos taurus
?
2.7.1.76
UTP + deoxyadenosine
90% of activity with ATP
641981
Bos taurus
UDP + dAMP
-
641981
Bos taurus
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.76
37
-
assay at
Bos taurus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.76
7.5
-
broad optimum, 10% difference in activity between pH 6.5 and pH 8.5
Bos taurus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.76
5.7
8.8
approx. half-maximal activity at pH 5.7, approx. 80% of maximal activity at pH 8.8
Bos taurus