EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.76 | Hg2+ | 0.01 mM, 25% inhibition, 0.1 mM, almost complete inhibition | Bos taurus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.76 | Ca2+ | activation, can replace Mg2+ | Bos taurus | |
2.7.1.76 | Mg2+ | required for activity | Bos taurus | |
2.7.1.76 | Mn2+ | activation, can replace Mg2+ | Bos taurus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.76 | 63000 | - |
gel filtration | Bos taurus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.76 | Bos taurus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.76 | streptomycin sulfate, protamine sulfate, ammonium sulfate, Sephadex G-150 | Bos taurus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.1.76 | thymus | - |
Bos taurus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.76 | 0.073 | - |
- |
Bos taurus |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.1.76 | -20°C, 60% glycerol, at least 3 months, slight loss of activity | Bos taurus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.76 | ATP + cytidine | approx. 18% of activity with deoxyadenosine | Bos taurus | ADP + CMP | - |
? | |
2.7.1.76 | ATP + deoxyadenosine | - |
Bos taurus | ADP + dAMP | - |
? | |
2.7.1.76 | ATP + deoxycytidine | approx. 12% of activity with deoxyadenosine | Bos taurus | ADP + dCMP | - |
? | |
2.7.1.76 | ATP + deoxyguanosine | - |
Bos taurus | ADP + dGMP | - |
? | |
2.7.1.76 | dTTP + deoxyadenosine | 75% of activity with ATP | Bos taurus | dTDP + dAMP | - |
? | |
2.7.1.76 | GTP + deoxyadenosine | - |
Bos taurus | GDP + dAMP | - |
? | |
2.7.1.76 | UTP + deoxyadenosine | 90% of activity with ATP | Bos taurus | UDP + dAMP | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.76 | 37 | - |
assay at | Bos taurus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.76 | 7.5 | - |
broad optimum, 10% difference in activity between pH 6.5 and pH 8.5 | Bos taurus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.76 | 5.7 | 8.8 | approx. half-maximal activity at pH 5.7, approx. 80% of maximal activity at pH 8.8 | Bos taurus |