BRENDA - Enzyme Database show

Substrate/inhibitor properties of human deoxycytidine kinase (dCK) and thymidine kinases (TK1 and TK2) towards the sugar moiety of nucleosides, including O'-alkyl analogues

Kierdaszuk, B.; Krawiec, K.; Kazimierczuk, Z.; Jacobsson, U.; Johansson, N.G.; Munch-Petersen, B.; Eriksson, S.; Shugar, D.; Nucleosides Nucleotides 18, 1883-1903 (1999)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
2'-O-methylcytidine
-
Homo sapiens
2.7.1.74
3'-O-methyl-2'deoxycytidine
-
Homo sapiens
2.7.1.74
5'-O-ethyl-2'-deoxycytidine
-
Homo sapiens
2.7.1.74
5'-O-methyl-2'-deoxycytidine
-
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.74
Homo sapiens
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
leukemia cell
from spleen
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
2',2'-difluoro-2'-deoxyguanosine + ATP
-
641965
Homo sapiens
ADP + 2',2'-difluoro-2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.74
2'-fluoro-2'-deoxyarabinosylcytidine + ATP
-
641965
Homo sapiens
ADP + 2'-fluoro-2'-deoxyarabinosylcytidine 5'-phosphate
-
-
-
?
2.7.1.74
2'-fluoro-2'-deoxycytidine + ATP
-
641965
Homo sapiens
ADP + 2'-fluoro-2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
2'-O-methyl-cytidine + ATP
-
641965
Homo sapiens
ADP + 2'-O-methyl-cytidine 5'-phosphate
-
-
-
?
2.7.1.74
2-chloro-2'-deoxyadenosine + NTP
-
641965
Homo sapiens
NDP + 2-chloro-2'-deoxy-AMP
-
-
-
?
2.7.1.74
3'-O-methyl-2'-deoxycytidine + ATP
-
641965
Homo sapiens
ADP + 3'-O-methyl-2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
9-(beta-D-arabinofuranosyl)-adenine + ATP
-
641965
Homo sapiens
9-(beta-D-arabinofuranosyl)-adenine 5'-phosphate + ADP
-
-
-
?
2.7.1.74
beta-D-3'-hydroxymethyl-2',3'dideoxycytidine + ATP
-
641965
Homo sapiens
ADP + beta-D-3'-hydroxymethyl-2',3'dideoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
NTP + cytosine arabinoside
-
641965
Homo sapiens
NDP + cytosine arabinoside 5'-monophosphate
-
-
-
?
2.7.1.74
NTP + deoxyadenosine
-
641965
Homo sapiens
NDP + dAMP
-
-
-
?
2.7.1.74
NTP + deoxycytidine
-
641965
Homo sapiens
NDP + dCMP
-
-
-
?
2.7.1.74
NTP + deoxyguanosine
-
641965
Homo sapiens
NDP + dGMP
-
-
-
?
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.74
0.02
-
3'-O-methyl-2'deoxycytidine
37°C, pH 7.6
Homo sapiens
2.7.1.74
0.032
-
5'-O-ethyl-2'-deoxycytidine
37°C, pH 7.6
Homo sapiens
2.7.1.74
0.23
-
5'-O-methyl-2'-deoxycytidine
37°C, pH 7.6
Homo sapiens
2.7.1.74
0.3
-
2'-O-methylcytidine
37°C, pH 7.6
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
2'-O-methylcytidine
-
Homo sapiens
2.7.1.74
3'-O-methyl-2'deoxycytidine
-
Homo sapiens
2.7.1.74
5'-O-ethyl-2'-deoxycytidine
-
Homo sapiens
2.7.1.74
5'-O-methyl-2'-deoxycytidine
-
Homo sapiens
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.74
0.02
-
3'-O-methyl-2'deoxycytidine
37°C, pH 7.6
Homo sapiens
2.7.1.74
0.032
-
5'-O-ethyl-2'-deoxycytidine
37°C, pH 7.6
Homo sapiens
2.7.1.74
0.23
-
5'-O-methyl-2'-deoxycytidine
37°C, pH 7.6
Homo sapiens
2.7.1.74
0.3
-
2'-O-methylcytidine
37°C, pH 7.6
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
leukemia cell
from spleen
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
2',2'-difluoro-2'-deoxyguanosine + ATP
-
641965
Homo sapiens
ADP + 2',2'-difluoro-2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.74
2'-fluoro-2'-deoxyarabinosylcytidine + ATP
-
641965
Homo sapiens
ADP + 2'-fluoro-2'-deoxyarabinosylcytidine 5'-phosphate
-
-
-
?
2.7.1.74
2'-fluoro-2'-deoxycytidine + ATP
-
641965
Homo sapiens
ADP + 2'-fluoro-2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
2'-O-methyl-cytidine + ATP
-
641965
Homo sapiens
ADP + 2'-O-methyl-cytidine 5'-phosphate
-
-
-
?
2.7.1.74
2-chloro-2'-deoxyadenosine + NTP
-
641965
Homo sapiens
NDP + 2-chloro-2'-deoxy-AMP
-
-
-
?
2.7.1.74
3'-O-methyl-2'-deoxycytidine + ATP
-
641965
Homo sapiens
ADP + 3'-O-methyl-2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
9-(beta-D-arabinofuranosyl)-adenine + ATP
-
641965
Homo sapiens
9-(beta-D-arabinofuranosyl)-adenine 5'-phosphate + ADP
-
-
-
?
2.7.1.74
beta-D-3'-hydroxymethyl-2',3'dideoxycytidine + ATP
-
641965
Homo sapiens
ADP + beta-D-3'-hydroxymethyl-2',3'dideoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
NTP + cytosine arabinoside
-
641965
Homo sapiens
NDP + cytosine arabinoside 5'-monophosphate
-
-
-
?
2.7.1.74
NTP + deoxyadenosine
-
641965
Homo sapiens
NDP + dAMP
-
-
-
?
2.7.1.74
NTP + deoxycytidine
-
641965
Homo sapiens
NDP + dCMP
-
-
-
?
2.7.1.74
NTP + deoxyguanosine
-
641965
Homo sapiens
NDP + dGMP
-
-
-
?