BRENDA - Enzyme Database show

Kinetic analysis of human deoxycytidine kinase with the true phosphate donor uridine triphosphate

Hughes, T.L.; Hahn, T.M.; Reynolds, K.K.; Shewach, D.S.; Biochemistry 36, 7540-7547 (1997)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.74
ATP
substrate activation, at high concentrations of deoxycytidine
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
dCMP
-
Homo sapiens
2.7.1.74
dCTP
-
Homo sapiens
2.7.1.74
deoxycytidine
at concentrations greater than 0.003 mM, UTP as substrate, noncompetitive
Homo sapiens
2.7.1.74
UDP
-
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.0005
-
UTP
cosubstrate 2'-deoxycytidine, 37°C, pH 7.4
Homo sapiens
2.7.1.74
0.0009
-
GTP
cosubstrate 2'-deoxycytidine, 37°C, pH 7.4
Homo sapiens
2.7.1.74
0.001
-
ATP
cosubstrate 2'-deoxycytidine, 37°C, pH 7.4
Homo sapiens
2.7.1.74
0.001
-
dTTP
cosubstrate 2'-deoxycytidine, 37°C, pH 7.4
Homo sapiens
2.7.1.74
0.0013
-
CTP
cosubstrate 2'-deoxycytidine, 37°C, pH 7.4
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.74
Homo sapiens
-
-
-
Reaction
EC Number
Reaction
Commentary
Organism
2.7.1.74
NTP + deoxycytidine = NDP + dCMP
mechanism; ordered A-B random B-Q reaction sequence
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
CTP + 2'-deoxycytidine
-
641958
Homo sapiens
CDP + 2'-deoxy-CMP
-
-
-
-
2.7.1.74
dTTP + 2'-deoxycytidine
-
641958
Homo sapiens
dTDP + 2'-deoxy-CMP
-
-
-
-
2.7.1.74
GTP + 2'-deoxycytidine
-
641958
Homo sapiens
GDP + 2'-deoxy-CMP
-
-
-
-
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.74
0.0004
-
UDP
cosubstrate UTP, 37°C, pH 7.4
Homo sapiens
2.7.1.74
0.0015
-
dCMP
cosubstrate UTP, 37°C, pH 7.4
Homo sapiens
2.7.1.74
0.0069
-
UDP
cosubstrate deoxycytidine, 37°C, pH 7.4
Homo sapiens
2.7.1.74
0.022
-
dCMP
cosubstrate deoxycytidine, 37°C, pH 7.4
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.74
ATP
substrate activation, at high concentrations of deoxycytidine
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
dCMP
-
Homo sapiens
2.7.1.74
dCTP
-
Homo sapiens
2.7.1.74
deoxycytidine
at concentrations greater than 0.003 mM, UTP as substrate, noncompetitive
Homo sapiens
2.7.1.74
UDP
-
Homo sapiens
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.74
0.0004
-
UDP
cosubstrate UTP, 37°C, pH 7.4
Homo sapiens
2.7.1.74
0.0015
-
dCMP
cosubstrate UTP, 37°C, pH 7.4
Homo sapiens
2.7.1.74
0.0069
-
UDP
cosubstrate deoxycytidine, 37°C, pH 7.4
Homo sapiens
2.7.1.74
0.022
-
dCMP
cosubstrate deoxycytidine, 37°C, pH 7.4
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.0005
-
UTP
cosubstrate 2'-deoxycytidine, 37°C, pH 7.4
Homo sapiens
2.7.1.74
0.0009
-
GTP
cosubstrate 2'-deoxycytidine, 37°C, pH 7.4
Homo sapiens
2.7.1.74
0.001
-
ATP
cosubstrate 2'-deoxycytidine, 37°C, pH 7.4
Homo sapiens
2.7.1.74
0.001
-
dTTP
cosubstrate 2'-deoxycytidine, 37°C, pH 7.4
Homo sapiens
2.7.1.74
0.0013
-
CTP
cosubstrate 2'-deoxycytidine, 37°C, pH 7.4
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
CTP + 2'-deoxycytidine
-
641958
Homo sapiens
CDP + 2'-deoxy-CMP
-
-
-
-
2.7.1.74
dTTP + 2'-deoxycytidine
-
641958
Homo sapiens
dTDP + 2'-deoxy-CMP
-
-
-
-
2.7.1.74
GTP + 2'-deoxycytidine
-
641958
Homo sapiens
GDP + 2'-deoxy-CMP
-
-
-
-