BRENDA - Enzyme Database show

Affinity purification of human deoxycytidine kinase: avoidance of structural and kinetic artifacts arising from limited proteolysis

Kim, M.Y.; Ikeda, S.; Ives, D.H.; Biochem. Biophys. Res. Commun. 156, 92-98 (1988)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.0033
0.0093
deoxycytidine
-
Homo sapiens
2.7.1.74
0.64
-
deoxyguanosine
37°C, pH 7.5
Homo sapiens
2.7.1.74
0.89
-
deoxyadenosine
37°C, pH 7.5
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.74
59300
-
sedimentation equilibrium determination, PAGE, both in the presence of protease inhibitors
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.74
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.74
affinity chromatography
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
leukemia cell
leukemic T-lymphoblasts
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
-
641944
Homo sapiens
ADP + 5'-dCMP
-
-
-
?
2.7.1.74
CTP + 2'-deoxycytidine
-
641944
Homo sapiens
CDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
dGTP + 2'-deoxycytidine
-
641944
Homo sapiens
dGDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
dTTP + 2'-deoxycytidine
-
641944
Homo sapiens
dTDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
GTP + 2'-deoxycytidine
-
641944
Homo sapiens
GDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
additional information
purine deoxynucleoside activity inseparably associated with deoxycytidine kinase protein
641944
Homo sapiens
?
-
-
-
-
2.7.1.74
NTP + deoxyadenosine
-
641944
Homo sapiens
NDP + dAMP
-
-
-
?
2.7.1.74
NTP + deoxycytidine
no substrate: dCTP
641944
Homo sapiens
NDP + dCMP
-
-
-
?
2.7.1.74
NTP + deoxyguanosine
-
641944
Homo sapiens
NDP + dGMP
-
-
-
?
2.7.1.74
UTP + 2'-deoxycytidine
-
641944
Homo sapiens
UDP + 2'-deoxy-CMP
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.1.74
monomer
-
Homo sapiens
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.74
37
-
assay at
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.0033
0.0093
deoxycytidine
-
Homo sapiens
2.7.1.74
0.64
-
deoxyguanosine
37°C, pH 7.5
Homo sapiens
2.7.1.74
0.89
-
deoxyadenosine
37°C, pH 7.5
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.74
59300
-
sedimentation equilibrium determination, PAGE, both in the presence of protease inhibitors
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.74
affinity chromatography
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
leukemia cell
leukemic T-lymphoblasts
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
-
641944
Homo sapiens
ADP + 5'-dCMP
-
-
-
?
2.7.1.74
CTP + 2'-deoxycytidine
-
641944
Homo sapiens
CDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
dGTP + 2'-deoxycytidine
-
641944
Homo sapiens
dGDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
dTTP + 2'-deoxycytidine
-
641944
Homo sapiens
dTDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
GTP + 2'-deoxycytidine
-
641944
Homo sapiens
GDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
additional information
purine deoxynucleoside activity inseparably associated with deoxycytidine kinase protein
641944
Homo sapiens
?
-
-
-
-
2.7.1.74
NTP + deoxyadenosine
-
641944
Homo sapiens
NDP + dAMP
-
-
-
?
2.7.1.74
NTP + deoxycytidine
no substrate: dCTP
641944
Homo sapiens
NDP + dCMP
-
-
-
?
2.7.1.74
NTP + deoxyguanosine
-
641944
Homo sapiens
NDP + dGMP
-
-
-
?
2.7.1.74
UTP + 2'-deoxycytidine
-
641944
Homo sapiens
UDP + 2'-deoxy-CMP
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.1.74
monomer
-
Homo sapiens
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.74
37
-
assay at
Homo sapiens