BRENDA - Enzyme Database show

Deoxycytidine kinase from human leukemic spleen: preparation and characteristics of homogeneous enzyme

Bohman, C.; Eriksson, S.; Biochemistry 27, 4258-4265 (1988)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.74
dithiothreitol
activation
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
dCTP
kinetics
Homo sapiens
2.7.1.74
deoxyadenosine
deoxycytidine
Homo sapiens
2.7.1.74
deoxycytidine
deoxyadenosine as substrate; deoxyguanosine as substrate
Homo sapiens
2.7.1.74
deoxyguanosine
deoxycytidine as substrate
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
additional information
-
additional information
-
Homo sapiens
2.7.1.74
0.0008
0.0015
deoxycytidine
pH 8.0, 37°C
Homo sapiens
2.7.1.74
0.02
0.04
cytosine arabinoside
pH 8.0, 37°C
Homo sapiens
2.7.1.74
0.12
-
deoxyadenosine
pH 8.0, 37°C
Homo sapiens
2.7.1.74
0.15
-
deoxyguanosine
pH 8.0, 37°C
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.74
mitochondrion
not
Homo sapiens
5739
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
Mg2+/ATP complex is the reactive species
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.74
30000
-
2 * 30000, SDS-PAGE
Homo sapiens
2.7.1.74
61000
-
gel filtration, glycerol gradient centrifugation
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.74
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.74
affinity chromatography; spleen
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
leukemia cell
from spleen; leukemic T-lymphoblasts
Homo sapiens
-
2.7.1.74
additional information
very low activity in normal spleens and placentas
Homo sapiens
-
2.7.1.74
spleen
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.74
0.11
0.15
pH 8.0, 37°C
Homo sapiens
Storage Stability
EC Number
Storage Stability
Organism
2.7.1.74
-70°C, concentrated enzyme solution in Tris-buffer with MgCl2 and mercaptoethanol, at least 12 months
Homo sapiens
2.7.1.74
4°C, concentrated enzyme solution in Tris-buffer with MgCl2 and mercaptoethanol, at least 2 weeks
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
-
641943
Homo sapiens
ADP + 5'-dCMP
-
-
-
?
2.7.1.74
dTTP + 2'-deoxycytidine
-
641943
Homo sapiens
dTDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
additional information
presumably one common nucleoside acceptor site
641943
Homo sapiens
?
-
-
-
-
2.7.1.74
NTP + cytosine arabinoside
-
641943
Homo sapiens
NDP + cytosine arabinoside 5'-monophosphate
-
-
-
?
2.7.1.74
NTP + deoxyadenosine
-
641943
Homo sapiens
NDP + dAMP
-
-
-
?
2.7.1.74
NTP + deoxycytidine
-
641943
Homo sapiens
NDP + dCMP
-
-
-
?
2.7.1.74
NTP + deoxyguanosine
-
641943
Homo sapiens
NDP + dGMP
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.1.74
dimer
2 * 30000, SDS-PAGE
Homo sapiens
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.74
37
-
assay at
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.74
5.5
8.5
plateau, higher activity in Tris than in phosphate buffer
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.74
dithiothreitol
activation
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
dCTP
kinetics
Homo sapiens
2.7.1.74
deoxyadenosine
deoxycytidine
Homo sapiens
2.7.1.74
deoxycytidine
deoxyadenosine as substrate; deoxyguanosine as substrate
Homo sapiens
2.7.1.74
deoxyguanosine
deoxycytidine as substrate
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
additional information
-
additional information
-
Homo sapiens
2.7.1.74
0.0008
0.0015
deoxycytidine
pH 8.0, 37°C
Homo sapiens
2.7.1.74
0.02
0.04
cytosine arabinoside
pH 8.0, 37°C
Homo sapiens
2.7.1.74
0.12
-
deoxyadenosine
pH 8.0, 37°C
Homo sapiens
2.7.1.74
0.15
-
deoxyguanosine
pH 8.0, 37°C
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.74
mitochondrion
not
Homo sapiens
5739
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
Mg2+/ATP complex is the reactive species
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.74
30000
-
2 * 30000, SDS-PAGE
Homo sapiens
2.7.1.74
61000
-
gel filtration, glycerol gradient centrifugation
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.74
affinity chromatography; spleen
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
leukemia cell
from spleen; leukemic T-lymphoblasts
Homo sapiens
-
2.7.1.74
additional information
very low activity in normal spleens and placentas
Homo sapiens
-
2.7.1.74
spleen
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.74
0.11
0.15
pH 8.0, 37°C
Homo sapiens
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.1.74
-70°C, concentrated enzyme solution in Tris-buffer with MgCl2 and mercaptoethanol, at least 12 months
Homo sapiens
2.7.1.74
4°C, concentrated enzyme solution in Tris-buffer with MgCl2 and mercaptoethanol, at least 2 weeks
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
-
641943
Homo sapiens
ADP + 5'-dCMP
-
-
-
?
2.7.1.74
dTTP + 2'-deoxycytidine
-
641943
Homo sapiens
dTDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
additional information
presumably one common nucleoside acceptor site
641943
Homo sapiens
?
-
-
-
-
2.7.1.74
NTP + cytosine arabinoside
-
641943
Homo sapiens
NDP + cytosine arabinoside 5'-monophosphate
-
-
-
?
2.7.1.74
NTP + deoxyadenosine
-
641943
Homo sapiens
NDP + dAMP
-
-
-
?
2.7.1.74
NTP + deoxycytidine
-
641943
Homo sapiens
NDP + dCMP
-
-
-
?
2.7.1.74
NTP + deoxyguanosine
-
641943
Homo sapiens
NDP + dGMP
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.1.74
dimer
2 * 30000, SDS-PAGE
Homo sapiens
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.74
37
-
assay at
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.74
5.5
8.5
plateau, higher activity in Tris than in phosphate buffer
Homo sapiens