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Literature summary extracted from

  • Datta, N.S.; Shewach, D.S.; Mitchell, B.S.; Fox, I.H.
    Kinetic properties and inhibition of human T lymphoblast deoxycytidine kinase (1989), J. Biol. Chem., 264, 9359-9364.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.74 ADP kinetics Homo sapiens
2.7.1.74 CMP
-
 Homo sapiens
2.7.1.74 CTP
-
 Homo sapiens
2.7.1.74 Cytosine arabinoside monophosphate strong Homo sapiens
2.7.1.74 Cytosine arabinoside triphosphate
-
 Homo sapiens
2.7.1.74 dAMP strong Homo sapiens
2.7.1.74 dATP strong Homo sapiens
2.7.1.74 dCDP
-
 Homo sapiens
2.7.1.74 dCMP product inhibition, strong Homo sapiens
2.7.1.74 dCTP kinetics Homo sapiens
2.7.1.74 dGMP strong Homo sapiens
2.7.1.74 Dideoxyadenosine
-
 Homo sapiens
2.7.1.74 Dideoxycytidine
-
 Homo sapiens
2.7.1.74 dUMP strong Homo sapiens
2.7.1.74 dUTP
-
 Homo sapiens
2.7.1.74 GDP
-
 Homo sapiens
2.7.1.74 IMP
-
 Homo sapiens
2.7.1.74 P1,P4-diadenosine 5'-tetraphosphate not di-, tri- or pentaphosphate derivative Homo sapiens
2.7.1.74 Sodium diphosphate not monophosphate Homo sapiens
2.7.1.74 UDP strong Homo sapiens
2.7.1.74 UMP strong Homo sapiens
2.7.1.74 UTP
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.74 additional information
-
 additional information
-
 Homo sapiens
2.7.1.74 0.0008 0.0015 deoxycytidine 37°C, pH 7.0 Homo sapiens
2.7.1.74 0.02 0.04 cytosine arabinoside pH 7.0, 37°C Homo sapiens
2.7.1.74 0.33 0.43 deoxyguanosine 37°C, pH 7.0 Homo sapiens
2.7.1.74 0.45
-
 cytidine 37°C, pH 7.0 Homo sapiens
2.7.1.74 0.5
-
 deoxyadenosine 37°C, pH 7.0 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.74 Mg2+ Mg2+/ATP complex is the reactive species Homo sapiens
2.7.1.74 Mg2+ maximal activity at a ATP:Mg2+-ratio of 1:1 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.74 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.74
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.74 leukemia cell leukemic T-lymphoblasts Homo sapiens
-
2.7.1.74 leukemia cell cell line MOLT-4 Homo sapiens
-
2.7.1.74 MOLT-4 cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.74 8
-
 37°C, pH 7.0 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.74 ATP + 2'-deoxycytidine
-
 Homo sapiens ADP + 5'-dCMP
-
 ?
2.7.1.74 ATP + cytidine
-
 Homo sapiens ADP + ?
-
 ?
2.7.1.74 NTP + cytosine arabinoside
-
 Homo sapiens NDP + cytosine arabinoside 5'-monophosphate
-
 ?
2.7.1.74 NTP + deoxyadenosine
-
 Homo sapiens NDP + dAMP
-
 ?
2.7.1.74 NTP + deoxycytidine enzyme shows little specificity towards phosphate donor Homo sapiens NDP + dCMP
-
 ?
2.7.1.74 NTP + deoxyguanosine
-
 Homo sapiens NDP + dGMP
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.74 37
-
 assay at Homo sapiens