BRENDA - Enzyme Database

Kinetic properties and inhibition of human T lymphoblast deoxycytidine kinase

Datta, N.S.; Shewach, D.S.; Mitchell, B.S.; Fox, I.H.; J. Biol. Chem. 264, 9359-9364 (1989)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
ADP
kinetics
Homo sapiens
2.7.1.74
CMP
-
Homo sapiens
2.7.1.74
CTP
-
Homo sapiens
2.7.1.74
Cytosine arabinoside monophosphate
strong
Homo sapiens
2.7.1.74
Cytosine arabinoside triphosphate
-
Homo sapiens
2.7.1.74
dAMP
strong
Homo sapiens
2.7.1.74
dATP
strong
Homo sapiens
2.7.1.74
dCDP
-
Homo sapiens
2.7.1.74
dCMP
product inhibition, strong
Homo sapiens
2.7.1.74
dCTP
kinetics
Homo sapiens
2.7.1.74
dGMP
strong
Homo sapiens
2.7.1.74
Dideoxyadenosine
-
Homo sapiens
2.7.1.74
Dideoxycytidine
-
Homo sapiens
2.7.1.74
dUMP
strong
Homo sapiens
2.7.1.74
dUTP
-
Homo sapiens
2.7.1.74
GDP
-
Homo sapiens
2.7.1.74
IMP
-
Homo sapiens
2.7.1.74
P1,P4-diadenosine 5'-tetraphosphate
not di-, tri- or pentaphosphate derivative
Homo sapiens
2.7.1.74
Sodium diphosphate
not monophosphate
Homo sapiens
2.7.1.74
UDP
strong
Homo sapiens
2.7.1.74
UMP
strong
Homo sapiens
2.7.1.74
UTP
-
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
additional information
-
additional information
-
Homo sapiens
2.7.1.74
0.0008
0.0015
deoxycytidine
37°C, pH 7.0
Homo sapiens
2.7.1.74
0.02
0.04
cytosine arabinoside
pH 7.0, 37°C
Homo sapiens
2.7.1.74
0.33
0.43
deoxyguanosine
37°C, pH 7.0
Homo sapiens
2.7.1.74
0.45
-
cytidine
37°C, pH 7.0
Homo sapiens
2.7.1.74
0.5
-
deoxyadenosine
37°C, pH 7.0
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
maximal activity at a ATP:Mg2+-ratio of 1:1; Mg2+/ATP complex is the reactive species
Homo sapiens
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.7.1.74
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
2.7.1.74
-
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
leukemia cell
cell line MOLT-4; leukemic T-lymphoblasts
Homo sapiens
-
2.7.1.74
MOLT-4 cell
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.74
8
-
37°C, pH 7.0
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.7.1.74
ATP + 2'-deoxycytidine
-
641941
Homo sapiens
ADP + 5'-dCMP
-
-
-
?
2.7.1.74
ATP + cytidine
-
641941
Homo sapiens
ADP + ?
-
-
-
?
2.7.1.74
NTP + cytosine arabinoside
-
641941
Homo sapiens
NDP + cytosine arabinoside 5'-monophosphate
-
-
-
?
2.7.1.74
NTP + deoxyadenosine
-
641941
Homo sapiens
NDP + dAMP
-
-
-
?
2.7.1.74
NTP + deoxycytidine
enzyme shows little specificity towards phosphate donor
641941
Homo sapiens
NDP + dCMP
-
-
-
?
2.7.1.74
NTP + deoxyguanosine
-
641941
Homo sapiens
NDP + dGMP
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.74
37
-
assay at
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
ADP
kinetics
Homo sapiens
2.7.1.74
CMP
-
Homo sapiens
2.7.1.74
CTP
-
Homo sapiens
2.7.1.74
Cytosine arabinoside monophosphate
strong
Homo sapiens
2.7.1.74
Cytosine arabinoside triphosphate
-
Homo sapiens
2.7.1.74
dAMP
strong
Homo sapiens
2.7.1.74
dATP
strong
Homo sapiens
2.7.1.74
dCDP
-
Homo sapiens
2.7.1.74
dCMP
product inhibition, strong
Homo sapiens
2.7.1.74
dCTP
kinetics
Homo sapiens
2.7.1.74
dGMP
strong
Homo sapiens
2.7.1.74
Dideoxyadenosine
-
Homo sapiens
2.7.1.74
Dideoxycytidine
-
Homo sapiens
2.7.1.74
dUMP
strong
Homo sapiens
2.7.1.74
dUTP
-
Homo sapiens
2.7.1.74
GDP
-
Homo sapiens
2.7.1.74
IMP
-
Homo sapiens
2.7.1.74
P1,P4-diadenosine 5'-tetraphosphate
not di-, tri- or pentaphosphate derivative
Homo sapiens
2.7.1.74
Sodium diphosphate
not monophosphate
Homo sapiens
2.7.1.74
UDP
strong
Homo sapiens
2.7.1.74
UMP
strong
Homo sapiens
2.7.1.74
UTP
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
additional information
-
additional information
-
Homo sapiens
2.7.1.74
0.0008
0.0015
deoxycytidine
37°C, pH 7.0
Homo sapiens
2.7.1.74
0.02
0.04
cytosine arabinoside
pH 7.0, 37°C
Homo sapiens
2.7.1.74
0.33
0.43
deoxyguanosine
37°C, pH 7.0
Homo sapiens
2.7.1.74
0.45
-
cytidine
37°C, pH 7.0
Homo sapiens
2.7.1.74
0.5
-
deoxyadenosine
37°C, pH 7.0
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
maximal activity at a ATP:Mg2+-ratio of 1:1; Mg2+/ATP complex is the reactive species
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.74
-
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
leukemia cell
cell line MOLT-4; leukemic T-lymphoblasts
Homo sapiens
-
2.7.1.74
MOLT-4 cell
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.74
8
-
37°C, pH 7.0
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.7.1.74
ATP + 2'-deoxycytidine
-
641941
Homo sapiens
ADP + 5'-dCMP
-
-
-
?
2.7.1.74
ATP + cytidine
-
641941
Homo sapiens
ADP + ?
-
-
-
?
2.7.1.74
NTP + cytosine arabinoside
-
641941
Homo sapiens
NDP + cytosine arabinoside 5'-monophosphate
-
-
-
?
2.7.1.74
NTP + deoxyadenosine
-
641941
Homo sapiens
NDP + dAMP
-
-
-
?
2.7.1.74
NTP + deoxycytidine
enzyme shows little specificity towards phosphate donor
641941
Homo sapiens
NDP + dCMP
-
-
-
?
2.7.1.74
NTP + deoxyguanosine
-
641941
Homo sapiens
NDP + dGMP
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.74
37
-
assay at
Homo sapiens