BRENDA - Enzyme Database show

Purification and characterization of deoxycytidine kinase from acute myeloid leukemia cell mitochondria

Wang, L.M.; Kucera, G.L.; Capizzi, R.L.; Biochim. Biophys. Acta 1202, 309-316 (1993)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.74
deoxyadenosine
activation, deoxycytidine as substrate, mitochondrial isozyme
Homo sapiens
2.7.1.74
deoxyguanosine
activation, deoxycytidine as substrate, mitochondrial isozyme
Homo sapiens
2.7.1.74
TTP
stimulation, cytosolic isozyme I
Homo sapiens
2.7.1.74
UDP
stimulation, mitochondrial isozyme
Homo sapiens
2.7.1.74
Uridine arabinoside
activation, deoxycytidine as substrate, mitochondrial isozyme
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
Cytosine arabinoside triphosphate
strong
Homo sapiens
2.7.1.74
dCMP
only cytosolic isozyme I; product inhibition, strong
Homo sapiens
2.7.1.74
dCTP
cytosine arabinoside as substrate; isozyme II
Homo sapiens
2.7.1.74
deoxyadenosine
cytosolic isozyme I, weak
Homo sapiens
2.7.1.74
deoxycytidine
cytosine arabinoside as substrate
Homo sapiens
2.7.1.74
deoxyguanosine
deoxyguanosine as substrate, cytosolic isozyme I, weak
Homo sapiens
2.7.1.74
deoxythymidine
weak
Homo sapiens
2.7.1.74
dGDP
not
Homo sapiens
2.7.1.74
dTTP
only mitochondrial isozyme; strong
Homo sapiens
2.7.1.74
dUDP
only cytosolic isozyme I; strong
Homo sapiens
2.7.1.74
GTP
not, deoxyadenosine as substrate
Homo sapiens
2.7.1.74
UDP
only cytosolic isozyme I; strong
Homo sapiens
2.7.1.74
Uridine arabinoside
weak, cytosolic isozyme I
Homo sapiens
2.7.1.74
UTP
not mitochondrial isozyme
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.002
-
cytosine arabinoside
deoxycytidine, cytosolic isozyme I, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.007
-
cytosine arabinoside
cytosolic isozyme I, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.014
0.0167
deoxycytidine
mitochondrial isozyme, pH 7.5, 37°C; pH 7.5, 37°C
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.74
cytosol
2 isozymes: I and II
Homo sapiens
5829
-
2.7.1.74
mitochondrion
outer membrane
Homo sapiens
5739
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.74
52000
-
1 * 52000, SDS-PAGE, mitochondrial isozyme
Homo sapiens
2.7.1.74
70000
-
human cytoplasmic isozymes
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.74
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.74
affinity chromatography; mitochondrial isozyme (extracted with digitonin); partial
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
BG-1 cell
-
Homo sapiens
-
2.7.1.74
leukemia cell
from acute myeloid leukemia (AML) patients; from peripheral blood; ML-1 cells
Homo sapiens
-
2.7.1.74
ML-1 cell
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.74
0.048
-
mitochondrial isozyme, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.072
-
cytoplasmic isozyme I, pH 7.5, 37°C
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
best phosphate donor cytosolic isozyme II and mitochondrial isozyme
641938
Homo sapiens
ADP + 5'-dCMP
-
641938
Homo sapiens
?
2.7.1.74
CTP + 2'-deoxycytidine
as good as GTP, UTP, dATP, mitochondrial isozyme
641938
Homo sapiens
CDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
dGTP + 2'-deoxycytidine
cytosolic isozyme I
641938
Homo sapiens
dGDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
dGTP + 2'-deoxycytidine
poor substrate of mitochondrial isozyme
641938
Homo sapiens
dGDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
dUTP + 2'-deoxycytidine
best phosphate donor, cytosolic enzyme I, poor, mitochondrial isozyme
641938
Homo sapiens
dUDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
GTP + 2'-deoxycytidine
as good as dGTP, dTTP or dUTP, cytosolic isozyme I
641938
Homo sapiens
GDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
GTP + 2'-deoxycytidine
as good as CTP, dATP or UTP, mitochondrial isozyme
641938
Homo sapiens
GDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
additional information
several isozymes: cytosolic deoxycytidine kinase I and II, plus mitochondrial isozyme
641938
Homo sapiens
?
-
-
-
-
2.7.1.74
NTP + 2',2'-difluoro-2'-deoxycytidine
cytosolic isozyme I, not mitochondrial isozyme
641938
Homo sapiens
NDP + 2',2'-difluoro-2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
NTP + cytosine arabinoside
D-isomer
641938
Homo sapiens
NDP + cytosine arabinoside 5'-monophosphate
-
-
-
?
2.7.1.74
NTP + cytosine arabinoside
cytosolic isozyme I
641938
Homo sapiens
NDP + cytosine arabinoside 5'-monophosphate
-
-
-
?
2.7.1.74
NTP + cytosine arabinoside
no acceptor substrate for cytosolic isozyme II or mitochondrial isozyme
641938
Homo sapiens
NDP + cytosine arabinoside 5'-monophosphate
-
-
-
?
2.7.1.74
NTP + deoxyadenosine
cytosolic isozyme I
641938
Homo sapiens
NDP + dAMP
-
-
-
?
2.7.1.74
NTP + deoxyadenosine
not mitochondrial isozyme
641938
Homo sapiens
NDP + dAMP
-
-
-
?
2.7.1.74
NTP + deoxycytidine
no substrate: guanosine
641938
Homo sapiens
NDP + dCMP
-
641938
Homo sapiens
?
2.7.1.74
NTP + deoxycytidine
poor substrate: arabinosyl-CTP
641938
Homo sapiens
NDP + dCMP
-
641938
Homo sapiens
?
2.7.1.74
NTP + deoxycytidine
no substrate: uridine arabinoside, cytosine
641938
Homo sapiens
NDP + dCMP
-
641938
Homo sapiens
?
2.7.1.74
NTP + deoxycytidine
no substrate: adenosine, uridine
641938
Homo sapiens
NDP + dCMP
-
641938
Homo sapiens
?
2.7.1.74
NTP + deoxyguanosine
cytosolic isozyme I
641938
Homo sapiens
NDP + dGMP
-
-
-
?
2.7.1.74
NTP + deoxyguanosine
not mitochondrial isozyme
641938
Homo sapiens
NDP + dGMP
-
-
-
?
2.7.1.74
NTP + deoxythymidine
mitochondrial isozyme, better than deoxycytidine, not cytosolic isozyme I
641938
Homo sapiens
NDP + dTMP
-
-
-
?
2.7.1.74
NTP + deoxyuridine
mitochondrial isozyme, not cytosolic isozyme I
641938
Homo sapiens
NDP + dUMP
-
-
-
?
2.7.1.74
UTP + 2'-deoxycytidine
as good as CTP, GTP or dATP
641938
Homo sapiens
UDP + 2'-deoxy-CMP
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.1.74
monomer
1 * 52000, SDS-PAGE, mitochondrial isozyme
Homo sapiens
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.74
37
-
assay at
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.74
deoxyadenosine
activation, deoxycytidine as substrate, mitochondrial isozyme
Homo sapiens
2.7.1.74
deoxyguanosine
activation, deoxycytidine as substrate, mitochondrial isozyme
Homo sapiens
2.7.1.74
TTP
stimulation, cytosolic isozyme I
Homo sapiens
2.7.1.74
UDP
stimulation, mitochondrial isozyme
Homo sapiens
2.7.1.74
Uridine arabinoside
activation, deoxycytidine as substrate, mitochondrial isozyme
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
Cytosine arabinoside triphosphate
strong
Homo sapiens
2.7.1.74
dCMP
only cytosolic isozyme I; product inhibition, strong
Homo sapiens
2.7.1.74
dCTP
cytosine arabinoside as substrate; isozyme II
Homo sapiens
2.7.1.74
deoxyadenosine
cytosolic isozyme I, weak
Homo sapiens
2.7.1.74
deoxycytidine
cytosine arabinoside as substrate
Homo sapiens
2.7.1.74
deoxyguanosine
deoxyguanosine as substrate, cytosolic isozyme I, weak
Homo sapiens
2.7.1.74
deoxythymidine
weak
Homo sapiens
2.7.1.74
dGDP
not
Homo sapiens
2.7.1.74
dTTP
only mitochondrial isozyme; strong
Homo sapiens
2.7.1.74
dUDP
only cytosolic isozyme I; strong
Homo sapiens
2.7.1.74
GTP
not, deoxyadenosine as substrate
Homo sapiens
2.7.1.74
UDP
only cytosolic isozyme I; strong
Homo sapiens
2.7.1.74
Uridine arabinoside
weak, cytosolic isozyme I
Homo sapiens
2.7.1.74
UTP
not mitochondrial isozyme
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.002
-
cytosine arabinoside
deoxycytidine, cytosolic isozyme I, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.007
-
cytosine arabinoside
cytosolic isozyme I, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.014
0.0167
deoxycytidine
mitochondrial isozyme, pH 7.5, 37°C; pH 7.5, 37°C
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.74
cytosol
2 isozymes: I and II
Homo sapiens
5829
-
2.7.1.74
mitochondrion
outer membrane
Homo sapiens
5739
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.74
52000
-
1 * 52000, SDS-PAGE, mitochondrial isozyme
Homo sapiens
2.7.1.74
70000
-
human cytoplasmic isozymes
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.74
affinity chromatography; mitochondrial isozyme (extracted with digitonin); partial
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
BG-1 cell
-
Homo sapiens
-
2.7.1.74
leukemia cell
from acute myeloid leukemia (AML) patients; from peripheral blood; ML-1 cells
Homo sapiens
-
2.7.1.74
ML-1 cell
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.74
0.048
-
mitochondrial isozyme, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.072
-
cytoplasmic isozyme I, pH 7.5, 37°C
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
best phosphate donor cytosolic isozyme II and mitochondrial isozyme
641938
Homo sapiens
ADP + 5'-dCMP
-
641938
Homo sapiens
?
2.7.1.74
CTP + 2'-deoxycytidine
as good as GTP, UTP, dATP, mitochondrial isozyme
641938
Homo sapiens
CDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
dGTP + 2'-deoxycytidine
cytosolic isozyme I
641938
Homo sapiens
dGDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
dGTP + 2'-deoxycytidine
poor substrate of mitochondrial isozyme
641938
Homo sapiens
dGDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
dUTP + 2'-deoxycytidine
best phosphate donor, cytosolic enzyme I, poor, mitochondrial isozyme
641938
Homo sapiens
dUDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
GTP + 2'-deoxycytidine
as good as dGTP, dTTP or dUTP, cytosolic isozyme I
641938
Homo sapiens
GDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
GTP + 2'-deoxycytidine
as good as CTP, dATP or UTP, mitochondrial isozyme
641938
Homo sapiens
GDP + 2'-deoxy-CMP
-
-
-
?
2.7.1.74
additional information
several isozymes: cytosolic deoxycytidine kinase I and II, plus mitochondrial isozyme
641938
Homo sapiens
?
-
-
-
-
2.7.1.74
NTP + 2',2'-difluoro-2'-deoxycytidine
cytosolic isozyme I, not mitochondrial isozyme
641938
Homo sapiens
NDP + 2',2'-difluoro-2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
NTP + cytosine arabinoside
D-isomer
641938
Homo sapiens
NDP + cytosine arabinoside 5'-monophosphate
-
-
-
?
2.7.1.74
NTP + cytosine arabinoside
cytosolic isozyme I
641938
Homo sapiens
NDP + cytosine arabinoside 5'-monophosphate
-
-
-
?
2.7.1.74
NTP + cytosine arabinoside
no acceptor substrate for cytosolic isozyme II or mitochondrial isozyme
641938
Homo sapiens
NDP + cytosine arabinoside 5'-monophosphate
-
-
-
?
2.7.1.74
NTP + deoxyadenosine
cytosolic isozyme I
641938
Homo sapiens
NDP + dAMP
-
-
-
?
2.7.1.74
NTP + deoxyadenosine
not mitochondrial isozyme
641938
Homo sapiens
NDP + dAMP
-
-
-
?
2.7.1.74
NTP + deoxycytidine
no substrate: guanosine
641938
Homo sapiens
NDP + dCMP
-
641938
Homo sapiens
?
2.7.1.74
NTP + deoxycytidine
poor substrate: arabinosyl-CTP
641938
Homo sapiens
NDP + dCMP
-
641938
Homo sapiens
?
2.7.1.74
NTP + deoxycytidine
no substrate: uridine arabinoside, cytosine
641938
Homo sapiens
NDP + dCMP
-
641938
Homo sapiens
?
2.7.1.74
NTP + deoxycytidine
no substrate: adenosine, uridine
641938
Homo sapiens
NDP + dCMP
-
641938
Homo sapiens
?
2.7.1.74
NTP + deoxyguanosine
cytosolic isozyme I
641938
Homo sapiens
NDP + dGMP
-
-
-
?
2.7.1.74
NTP + deoxyguanosine
not mitochondrial isozyme
641938
Homo sapiens
NDP + dGMP
-
-
-
?
2.7.1.74
NTP + deoxythymidine
mitochondrial isozyme, better than deoxycytidine, not cytosolic isozyme I
641938
Homo sapiens
NDP + dTMP
-
-
-
?
2.7.1.74
NTP + deoxyuridine
mitochondrial isozyme, not cytosolic isozyme I
641938
Homo sapiens
NDP + dUMP
-
-
-
?
2.7.1.74
UTP + 2'-deoxycytidine
as good as CTP, GTP or dATP
641938
Homo sapiens
UDP + 2'-deoxy-CMP
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.1.74
monomer
1 * 52000, SDS-PAGE, mitochondrial isozyme
Homo sapiens
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.74
37
-
assay at
Homo sapiens