BRENDA - Enzyme Database show

Selective inactivation of the deoxyadenosine phosphorylating activity of pure human deoxycytidine kinase: stabilization of different forms of the enzyme by substrates and biological detergents

Kierdaszuk, B.; Eriksson, S.; Biochemistry 29, 4109-4114 (1990)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.74
CHAPS
slight stimulation, deoxycytidine as substrate
Homo sapiens
2.7.1.74
sodium cholate
slight stimulation, deoxycytidine as substrate
Homo sapiens
2.7.1.74
Zwittergent 3-14
slight stimulation, deoxycytidine as substrate
Homo sapiens
General Stability
EC Number
General Stability
Organism
2.7.1.74
bovine serum albumin stabilizes
Homo sapiens
2.7.1.74
dTTP, ATP or deoxycytidine stabilizes
Homo sapiens
2.7.1.76
stability of deoxyadenosine kinase activity differs appreciably from deoxycytidine kinase activity
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
additional information
deoxycytidine phosphorylating ability shows different inhibition pattern than deoxyadenosine activity
Homo sapiens
2.7.1.76
CHAPS
at critical micelle concentration
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
additional information
-
additional information
-
Homo sapiens
2.7.1.76
additional information
-
additional information
kinetic study
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
-
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.74
ATP + deoxycytidine
Homo sapiens
key anabolic enzyme for activation of purine or pyrimidine deoxynucleosides as well as cytidine arabinoside and other anti-tumor drugs
ADP + ?
-
-
?
2.7.1.76
ATP + deoxyadenosine
Homo sapiens
key anabolic enzyme for activation of purine and pyrimidine deoxyribonucleosides as well as cytosine arabinoside and other anti-tumour drugs
ADP + 5'-dAMP
-
Homo sapiens
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.74
Homo sapiens
-
-
-
2.7.1.76
Homo sapiens
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
leukemia cell
from spleen
Homo sapiens
-
2.7.1.74
spleen
-
Homo sapiens
-
2.7.1.76
spleen
leukemic
Homo sapiens
-
Storage Stability
EC Number
Storage Stability
Organism
2.7.1.74
4°C, t1/2: 7 days with and t1/2: 72 h without bovine serum albumin
Homo sapiens
2.7.1.76
4°C, t1/2: 84 h with and t1/2: 32 h without addition of bovine serum albumin
Homo sapiens
2.7.1.76
ATP, dTTP, deoxyadenosine or CHAPS stabilizes at 4°C
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
-
641937
Homo sapiens
ADP + 5'-dCMP
-
641937
Homo sapiens
?
2.7.1.74
ATP + deoxycytidine
key anabolic enzyme for activation of purine or pyrimidine deoxynucleosides as well as cytidine arabinoside and other anti-tumor drugs
641937
Homo sapiens
ADP + ?
-
-
-
?
2.7.1.74
additional information
multisubstrate enzyme, that also phosphorylates purine deoxyribonucleotides
641937
Homo sapiens
?
-
-
-
-
2.7.1.74
additional information
enzyme exists in different conformational states with different substrate kinetic properties
641937
Homo sapiens
?
-
-
-
-
2.7.1.74
NTP + deoxyadenosine
-
641937
Homo sapiens
NDP + dAMP
-
-
-
?
2.7.1.74
NTP + deoxycytidine
-
641937
Homo sapiens
NDP + dCMP
-
641937
Homo sapiens
?
2.7.1.76
ATP + deoxyadenosine
-
641937
Homo sapiens
ADP + dAMP
-
641937
Homo sapiens
?
2.7.1.76
ATP + deoxyadenosine
key anabolic enzyme for activation of purine and pyrimidine deoxyribonucleosides as well as cytosine arabinoside and other anti-tumour drugs
641937
Homo sapiens
ADP + 5'-dAMP
-
641937
Homo sapiens
?
2.7.1.76
ATP + deoxycytidine
deoxycytidine kinase is a multisubstrate enzyme that also phosphorylates deoxyadenosine, it exists in different conformational states with different substrate kinetic properties
641937
Homo sapiens
ADP + dCMP
-
641937
Homo sapiens
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.74
50
-
-
Homo sapiens
2.7.1.76
44
-
-
Homo sapiens
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.7.1.74
37
55
about half-maximal activity at 37°C and 55°C
Homo sapiens
2.7.1.76
27
50
approx. half-maximal activity at 27°C and 50°C
Homo sapiens
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.1.74
additional information
-
temperature stability of deoxycytidine kinase activity differs appreciably from deoxyadenosine kinase activity
Homo sapiens
2.7.1.74
37
-
at least 6 h stable
Homo sapiens
2.7.1.74
54
-
inactivation
Homo sapiens
2.7.1.76
additional information
-
temperature stability of deoxyadenosine kinase activity differs appreciably from deoxycytidine kinase activity
Homo sapiens
2.7.1.76
4
-
half-life of deoxyadenosine kinase activity: 1.5 days
Homo sapiens
2.7.1.76
37
-
t1/2: 2 h, 1 mM ATP, 0.5 mM dTTP, 1 mM deoxyadenosine or 0.25 3-[(3-cholamidopropyl)dimethylamino]-1-propanesulfonate stabilize
Homo sapiens
2.7.1.76
48
-
inactivation of deoxyadenosine kinase activity
Homo sapiens
2.7.1.76
50
-
80% loss of activity
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.74
CHAPS
slight stimulation, deoxycytidine as substrate
Homo sapiens
2.7.1.74
sodium cholate
slight stimulation, deoxycytidine as substrate
Homo sapiens
2.7.1.74
Zwittergent 3-14
slight stimulation, deoxycytidine as substrate
Homo sapiens
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.1.74
bovine serum albumin stabilizes
Homo sapiens
2.7.1.74
dTTP, ATP or deoxycytidine stabilizes
Homo sapiens
2.7.1.76
stability of deoxyadenosine kinase activity differs appreciably from deoxycytidine kinase activity
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
additional information
deoxycytidine phosphorylating ability shows different inhibition pattern than deoxyadenosine activity
Homo sapiens
2.7.1.76
CHAPS
at critical micelle concentration
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
additional information
-
additional information
-
Homo sapiens
2.7.1.76
additional information
-
additional information
kinetic study
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
-
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.74
ATP + deoxycytidine
Homo sapiens
key anabolic enzyme for activation of purine or pyrimidine deoxynucleosides as well as cytidine arabinoside and other anti-tumor drugs
ADP + ?
-
-
?
2.7.1.76
ATP + deoxyadenosine
Homo sapiens
key anabolic enzyme for activation of purine and pyrimidine deoxyribonucleosides as well as cytosine arabinoside and other anti-tumour drugs
ADP + 5'-dAMP
-
Homo sapiens
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
leukemia cell
from spleen
Homo sapiens
-
2.7.1.74
spleen
-
Homo sapiens
-
2.7.1.76
spleen
leukemic
Homo sapiens
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.1.74
4°C, t1/2: 7 days with and t1/2: 72 h without bovine serum albumin
Homo sapiens
2.7.1.76
4°C, t1/2: 84 h with and t1/2: 32 h without addition of bovine serum albumin
Homo sapiens
2.7.1.76
ATP, dTTP, deoxyadenosine or CHAPS stabilizes at 4°C
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
-
641937
Homo sapiens
ADP + 5'-dCMP
-
641937
Homo sapiens
?
2.7.1.74
ATP + deoxycytidine
key anabolic enzyme for activation of purine or pyrimidine deoxynucleosides as well as cytidine arabinoside and other anti-tumor drugs
641937
Homo sapiens
ADP + ?
-
-
-
?
2.7.1.74
additional information
multisubstrate enzyme, that also phosphorylates purine deoxyribonucleotides
641937
Homo sapiens
?
-
-
-
-
2.7.1.74
additional information
enzyme exists in different conformational states with different substrate kinetic properties
641937
Homo sapiens
?
-
-
-
-
2.7.1.74
NTP + deoxyadenosine
-
641937
Homo sapiens
NDP + dAMP
-
-
-
?
2.7.1.74
NTP + deoxycytidine
-
641937
Homo sapiens
NDP + dCMP
-
641937
Homo sapiens
?
2.7.1.76
ATP + deoxyadenosine
-
641937
Homo sapiens
ADP + dAMP
-
641937
Homo sapiens
?
2.7.1.76
ATP + deoxyadenosine
key anabolic enzyme for activation of purine and pyrimidine deoxyribonucleosides as well as cytosine arabinoside and other anti-tumour drugs
641937
Homo sapiens
ADP + 5'-dAMP
-
641937
Homo sapiens
?
2.7.1.76
ATP + deoxycytidine
deoxycytidine kinase is a multisubstrate enzyme that also phosphorylates deoxyadenosine, it exists in different conformational states with different substrate kinetic properties
641937
Homo sapiens
ADP + dCMP
-
641937
Homo sapiens
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.74
50
-
-
Homo sapiens
2.7.1.76
44
-
-
Homo sapiens
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.7.1.74
37
55
about half-maximal activity at 37°C and 55°C
Homo sapiens
2.7.1.76
27
50
approx. half-maximal activity at 27°C and 50°C
Homo sapiens
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.1.74
additional information
-
temperature stability of deoxycytidine kinase activity differs appreciably from deoxyadenosine kinase activity
Homo sapiens
2.7.1.74
37
-
at least 6 h stable
Homo sapiens
2.7.1.74
54
-
inactivation
Homo sapiens
2.7.1.76
additional information
-
temperature stability of deoxyadenosine kinase activity differs appreciably from deoxycytidine kinase activity
Homo sapiens
2.7.1.76
4
-
half-life of deoxyadenosine kinase activity: 1.5 days
Homo sapiens
2.7.1.76
37
-
t1/2: 2 h, 1 mM ATP, 0.5 mM dTTP, 1 mM deoxyadenosine or 0.25 3-[(3-cholamidopropyl)dimethylamino]-1-propanesulfonate stabilize
Homo sapiens
2.7.1.76
48
-
inactivation of deoxyadenosine kinase activity
Homo sapiens
2.7.1.76
50
-
80% loss of activity
Homo sapiens