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Literature summary extracted from

  • Ives, D.H.; Wang, S.M.
    Deoxycytidine kinase from calf thymus (1978), Methods Enzymol., 51, 337-345.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.74 bovine serum albumin stabilizes Bos taurus
2.7.1.74 dithiothreitol stabilizes Bos taurus
2.7.1.74 SH-reducing agents stabilize Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.74 dCMP dTTP reverses; product inhibition, strong Bos taurus
2.7.1.74 dCTP no reversion by beta-gamma-methylene diphosphonate analogue of dTTP Bos taurus
2.7.1.74 dTDP
-
 Bos taurus
2.7.1.74 Nucleoside analogs related to deoxycytidine, deoxyadenosine or deoxyguanosine Bos taurus
2.7.1.74 UDP
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.74 additional information
-
 additional information linear kinetics with deoxyguanosine or cytidine, bimodal kinetics with deoxycytidine as substrate Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.74 cytosol
-
 Bos taurus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.74 Ca2+ activation Bos taurus
2.7.1.74 Co2+ activation Bos taurus
2.7.1.74 Fe2+ activation Bos taurus
2.7.1.74 Mg2+
-
 Bos taurus
2.7.1.74 Mn2+ requirement Bos taurus
2.7.1.74 Ni2+ activation Bos taurus
2.7.1.74 Sr2+ activation Bos taurus
2.7.1.74 Zn2+ activation Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.74 56000
-
 gel filtration, equilibrium density and sucrose density gradient centrifugation Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.74 ATP + deoxycytidine Bos taurus
-
 ADP + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.74 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.74 preparative PAGE Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.74 thymus
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.74 0.0206
-
 37°C, pH 8.0 Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.7.1.74 -20°C, in 5 mM DTT and 50% glycerol, stable Bos taurus
2.7.1.74 dithiothreitol is more effective than 2-mercaptoethanol in stabilizing during storage Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.74 ATP + 2'-deoxycytidine
-
 Bos taurus ADP + 5'-dCMP
-
 ?
2.7.1.74 ATP + cytidine
-
 Bos taurus ADP + ?
-
 ?
2.7.1.74 ATP + deoxycytidine
-
 Bos taurus ADP + ?
-
 ?
2.7.1.74 NTP + deoxyadenosine
-
 Bos taurus NDP + dAMP
-
 ?
2.7.1.74 NTP + deoxycytidine no substrate: beta-gamma-methylene diphosphonate analogue of dTTP Bos taurus NDP + dCMP
-
 ?
2.7.1.74 NTP + deoxycytidine best acceptor substrate at low substrate concentrations Bos taurus NDP + dCMP
-
 ?
2.7.1.74 NTP + deoxyguanosine
-
 Bos taurus NDP + dGMP
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.74 37
-
 assay at Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.74 60
-
 t1/2: 7.9 min at pH 8, t1/2: 2 min at pH 6, unstable at pH 10 Bos taurus
2.7.1.74 60
-
 ATP, dCTP or glycerol stabilizes against heat inactivation Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.74 6 10 nearly flat response to pH-variations Bos taurus