BRENDA - Enzyme Database show

Deoxycytidine kinase from calf thymus

Ives, D.H.; Wang, S.M.; Methods Enzymol. 51, 337-345 (1978)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.7.1.74
bovine serum albumin stabilizes
Bos taurus
2.7.1.74
dithiothreitol stabilizes
Bos taurus
2.7.1.74
SH-reducing agents stabilize
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
dCMP
dTTP reverses; product inhibition, strong
Bos taurus
2.7.1.74
dCTP
no reversion by beta-gamma-methylene diphosphonate analogue of dTTP
Bos taurus
2.7.1.74
dTDP
-
Bos taurus
2.7.1.74
Nucleoside analogs
related to deoxycytidine, deoxyadenosine or deoxyguanosine
Bos taurus
2.7.1.74
UDP
-
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
additional information
-
additional information
linear kinetics with deoxyguanosine or cytidine, bimodal kinetics with deoxycytidine as substrate
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.74
cytosol
-
Bos taurus
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Ca2+
activation
Bos taurus
2.7.1.74
Co2+
activation
Bos taurus
2.7.1.74
Fe2+
activation
Bos taurus
2.7.1.74
Mg2+
-
Bos taurus
2.7.1.74
Mn2+
requirement
Bos taurus
2.7.1.74
Ni2+
activation
Bos taurus
2.7.1.74
Sr2+
activation
Bos taurus
2.7.1.74
Zn2+
activation
Bos taurus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.74
56000
-
gel filtration, equilibrium density and sucrose density gradient centrifugation
Bos taurus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.74
ATP + deoxycytidine
Bos taurus
-
ADP + ?
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.74
Bos taurus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.74
preparative PAGE
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
thymus
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.74
0.0206
-
37°C, pH 8.0
Bos taurus
Storage Stability
EC Number
Storage Stability
Organism
2.7.1.74
-20°C, in 5 mM DTT and 50% glycerol, stable
Bos taurus
2.7.1.74
dithiothreitol is more effective than 2-mercaptoethanol in stabilizing during storage
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
-
641936
Bos taurus
ADP + 5'-dCMP
-
641936
Bos taurus
?
2.7.1.74
ATP + cytidine
-
641936
Bos taurus
ADP + ?
-
-
-
?
2.7.1.74
ATP + deoxycytidine
-
641936
Bos taurus
ADP + ?
-
-
-
?
2.7.1.74
NTP + deoxyadenosine
-
641936
Bos taurus
NDP + dAMP
-
-
-
?
2.7.1.74
NTP + deoxycytidine
no substrate: beta-gamma-methylene diphosphonate analogue of dTTP
641936
Bos taurus
NDP + dCMP
-
641936
Bos taurus
?
2.7.1.74
NTP + deoxycytidine
best acceptor substrate at low substrate concentrations
641936
Bos taurus
NDP + dCMP
-
641936
Bos taurus
?
2.7.1.74
NTP + deoxyguanosine
-
641936
Bos taurus
NDP + dGMP
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.74
37
-
assay at
Bos taurus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.1.74
60
-
ATP, dCTP or glycerol stabilizes against heat inactivation; t1/2: 7.9 min at pH 8, t1/2: 2 min at pH 6, unstable at pH 10
Bos taurus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.74
6
10
nearly flat response to pH-variations
Bos taurus
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.1.74
bovine serum albumin stabilizes
Bos taurus
2.7.1.74
dithiothreitol stabilizes
Bos taurus
2.7.1.74
SH-reducing agents stabilize
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.74
dCMP
dTTP reverses; product inhibition, strong
Bos taurus
2.7.1.74
dCTP
no reversion by beta-gamma-methylene diphosphonate analogue of dTTP
Bos taurus
2.7.1.74
dTDP
-
Bos taurus
2.7.1.74
Nucleoside analogs
related to deoxycytidine, deoxyadenosine or deoxyguanosine
Bos taurus
2.7.1.74
UDP
-
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
additional information
-
additional information
linear kinetics with deoxyguanosine or cytidine, bimodal kinetics with deoxycytidine as substrate
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.74
cytosol
-
Bos taurus
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Ca2+
activation
Bos taurus
2.7.1.74
Co2+
activation
Bos taurus
2.7.1.74
Fe2+
activation
Bos taurus
2.7.1.74
Mg2+
-
Bos taurus
2.7.1.74
Mn2+
requirement
Bos taurus
2.7.1.74
Ni2+
activation
Bos taurus
2.7.1.74
Sr2+
activation
Bos taurus
2.7.1.74
Zn2+
activation
Bos taurus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.74
56000
-
gel filtration, equilibrium density and sucrose density gradient centrifugation
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.74
ATP + deoxycytidine
Bos taurus
-
ADP + ?
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.74
preparative PAGE
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
thymus
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.74
0.0206
-
37°C, pH 8.0
Bos taurus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.1.74
-20°C, in 5 mM DTT and 50% glycerol, stable
Bos taurus
2.7.1.74
dithiothreitol is more effective than 2-mercaptoethanol in stabilizing during storage
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
-
641936
Bos taurus
ADP + 5'-dCMP
-
641936
Bos taurus
?
2.7.1.74
ATP + cytidine
-
641936
Bos taurus
ADP + ?
-
-
-
?
2.7.1.74
ATP + deoxycytidine
-
641936
Bos taurus
ADP + ?
-
-
-
?
2.7.1.74
NTP + deoxyadenosine
-
641936
Bos taurus
NDP + dAMP
-
-
-
?
2.7.1.74
NTP + deoxycytidine
no substrate: beta-gamma-methylene diphosphonate analogue of dTTP
641936
Bos taurus
NDP + dCMP
-
641936
Bos taurus
?
2.7.1.74
NTP + deoxycytidine
best acceptor substrate at low substrate concentrations
641936
Bos taurus
NDP + dCMP
-
641936
Bos taurus
?
2.7.1.74
NTP + deoxyguanosine
-
641936
Bos taurus
NDP + dGMP
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.74
37
-
assay at
Bos taurus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.1.74
60
-
ATP, dCTP or glycerol stabilizes against heat inactivation; t1/2: 7.9 min at pH 8, t1/2: 2 min at pH 6, unstable at pH 10
Bos taurus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.74
6
10
nearly flat response to pH-variations
Bos taurus