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Literature summary extracted from

  • Durham, J.P.; Ives, D.H.
    Deoxycytidine kinase. II. Purification and general properties of the calf thymus enzyme (1970), J. Biol. Chem., 245, 2276-2284.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.74 bovine serum albumin activation Bos taurus
2.7.1.74 dithiothreitol activation Bos taurus

General Stability

EC Number General Stability Organism
2.7.1.74 2-mercaptoethanol stabilizes Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.74 ADP not Bos taurus
2.7.1.74 CMP not Bos taurus
2.7.1.74 cytidine cytosine arabinoside as substrate Bos taurus
2.7.1.74 cytosine arabinoside deoxyguanosine as substrate Bos taurus
2.7.1.74 dAMP not Bos taurus
2.7.1.74 dCDP
-
Bos taurus
2.7.1.74 dCMP
-
Bos taurus
2.7.1.74 dCTP reversible by dTTP; reversible by UTP, dUTP Bos taurus
2.7.1.74 deoxyadenosine cytosine arabinoside or deoxyguanosine as substrate Bos taurus
2.7.1.74 deoxycytidine cytosine arabinoside as substrate; deoxyadenosine as substrate; deoxyguanosine as substrate Bos taurus
2.7.1.74 deoxyguanosine cytosine arabinoside as substrate Bos taurus
2.7.1.74 deoxythymidine cytosolic isozyme I, not Bos taurus
2.7.1.74 dTDP
-
Bos taurus
2.7.1.74 dUMP not Bos taurus
2.7.1.74 GDP not Bos taurus
2.7.1.74 GSSG thiol reagents protect or reverse Bos taurus
2.7.1.74 HgCl2 strong, thiol reagents protect Bos taurus
2.7.1.74 additional information little or no inhibition by AMP, dTMP, adenosine, guanosine; little or no inhibition by deoxyuridine Bos taurus
2.7.1.74 N-ethylmaleimide thiol reagents protect or reverse Bos taurus
2.7.1.74 p-chloromercuribenzoate thiol reagents protect or reverse Bos taurus
2.7.1.74 UDP
-
Bos taurus
2.7.1.74 UMP not Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.74 Ca2+ activation Bos taurus
2.7.1.74 Ca2+ about 55% as efficient as Mg2+ Bos taurus
2.7.1.74 CdCl2 activation, in the absence of dithiothreitol Bos taurus
2.7.1.74 Co2+ activation Bos taurus
2.7.1.74 Co2+ about 40% as efficient as Mg2+ Bos taurus
2.7.1.74 Fe2+ activation Bos taurus
2.7.1.74 Fe2+ about 45% as efficient as Mg2+ Bos taurus
2.7.1.74 Mg2+
-
Bos taurus
2.7.1.74 Mn2+ requirement Bos taurus
2.7.1.74 Mn2+ about 80% as efficient as Mg2+ Bos taurus
2.7.1.74 additional information no activation by Ba2+, Cu2+ Bos taurus
2.7.1.74 Ni2+ activation Bos taurus
2.7.1.74 Ni2+ about 20% as efficient as Mg2+ Bos taurus
2.7.1.74 Sr2+ activation Bos taurus
2.7.1.74 Sr2+ about 15% as efficient as Mg2+ Bos taurus
2.7.1.74 Zn2+ activation Bos taurus
2.7.1.74 Zn2+ about 40% as efficient as Mg2+ Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.74 56000
-
gel filtration, equilibrium density and sucrose density gradient centrifugation Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.74 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.74
-
Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.74 thymus
-
Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.74 0.012
-
37°C, pH 8.0 Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.7.1.74 -30°C, several months in the presence of 2-mercaptoethanol Bos taurus
2.7.1.74 4°C, if purified in the absence of 2-mercaptoethanol it loses most of its activity within 2 days, DTT restores Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.74 ATP + 2'-deoxycytidine in the presence of DTT less effective than dUTP, UTP, dTTP or GTP, without DTT only less effective than dUTP Bos taurus ADP + 5'-dCMP
-
?
2.7.1.74 dTTP + 2'-deoxycytidine
-
Bos taurus dTDP + 2'-deoxy-CMP
-
?
2.7.1.74 dUTP + 2'-deoxycytidine best phosphate donor Bos taurus dUDP + 2'-deoxy-CMP
-
?
2.7.1.74 GTP + 2'-deoxycytidine
-
Bos taurus GDP + 2'-deoxy-CMP
-
?
2.7.1.74 NTP + cytosine arabinoside
-
Bos taurus NDP + cytosine arabinoside 5'-monophosphate
-
?
2.7.1.74 NTP + deoxyadenosine
-
Bos taurus NDP + dAMP
-
?
2.7.1.74 NTP + deoxycytidine
-
Bos taurus NDP + dCMP
-
?
2.7.1.74 NTP + deoxyguanosine
-
Bos taurus NDP + dGMP
-
?
2.7.1.74 UTP + 2'-deoxycytidine
-
Bos taurus UDP + 2'-deoxy-CMP
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.74 37
-
assay at Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.74 22
-
at least 24 h, in the presence of dithiothreitol Bos taurus
2.7.1.74 60
-
dCTP enhances heat stability at pH 6, dithiothreitol slightly, not deoxycytidine or other nucleosides Bos taurus
2.7.1.74 60
-
t1/2: 7.9 min at pH 8, t1/2: 2 min at pH 6, unstable at pH 10 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.74 6 10 broad plateau, with very slight optima at pH 6 and 10 Bos taurus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.74 6
-
t1/2: 2 min at 60°C Bos taurus
2.7.1.74 8
-
moderately stable, t1/2: 7.9 min at 60°C Bos taurus
2.7.1.74 10
-
unstable at 60°C Bos taurus