EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.74 | bovine serum albumin | activation | Bos taurus | |
2.7.1.74 | dithiothreitol | activation | Bos taurus |
EC Number | General Stability | Organism |
---|---|---|
2.7.1.74 | 2-mercaptoethanol stabilizes | Bos taurus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.74 | ADP | not | Bos taurus | |
2.7.1.74 | CMP | not | Bos taurus | |
2.7.1.74 | cytidine | cytosine arabinoside as substrate | Bos taurus | |
2.7.1.74 | cytosine arabinoside | deoxyguanosine as substrate | Bos taurus | |
2.7.1.74 | dAMP | not | Bos taurus | |
2.7.1.74 | dCDP | - |
Bos taurus | |
2.7.1.74 | dCMP | - |
Bos taurus | |
2.7.1.74 | dCTP | reversible by dTTP; reversible by UTP, dUTP | Bos taurus | |
2.7.1.74 | deoxyadenosine | cytosine arabinoside or deoxyguanosine as substrate | Bos taurus | |
2.7.1.74 | deoxycytidine | cytosine arabinoside as substrate; deoxyadenosine as substrate; deoxyguanosine as substrate | Bos taurus | |
2.7.1.74 | deoxyguanosine | cytosine arabinoside as substrate | Bos taurus | |
2.7.1.74 | deoxythymidine | cytosolic isozyme I, not | Bos taurus | |
2.7.1.74 | dTDP | - |
Bos taurus | |
2.7.1.74 | dUMP | not | Bos taurus | |
2.7.1.74 | GDP | not | Bos taurus | |
2.7.1.74 | GSSG | thiol reagents protect or reverse | Bos taurus | |
2.7.1.74 | HgCl2 | strong, thiol reagents protect | Bos taurus | |
2.7.1.74 | additional information | little or no inhibition by AMP, dTMP, adenosine, guanosine; little or no inhibition by deoxyuridine | Bos taurus | |
2.7.1.74 | N-ethylmaleimide | thiol reagents protect or reverse | Bos taurus | |
2.7.1.74 | p-chloromercuribenzoate | thiol reagents protect or reverse | Bos taurus | |
2.7.1.74 | UDP | - |
Bos taurus | |
2.7.1.74 | UMP | not | Bos taurus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.74 | Ca2+ | activation | Bos taurus | |
2.7.1.74 | Ca2+ | about 55% as efficient as Mg2+ | Bos taurus | |
2.7.1.74 | CdCl2 | activation, in the absence of dithiothreitol | Bos taurus | |
2.7.1.74 | Co2+ | activation | Bos taurus | |
2.7.1.74 | Co2+ | about 40% as efficient as Mg2+ | Bos taurus | |
2.7.1.74 | Fe2+ | activation | Bos taurus | |
2.7.1.74 | Fe2+ | about 45% as efficient as Mg2+ | Bos taurus | |
2.7.1.74 | Mg2+ | - |
Bos taurus | |
2.7.1.74 | Mn2+ | requirement | Bos taurus | |
2.7.1.74 | Mn2+ | about 80% as efficient as Mg2+ | Bos taurus | |
2.7.1.74 | additional information | no activation by Ba2+, Cu2+ | Bos taurus | |
2.7.1.74 | Ni2+ | activation | Bos taurus | |
2.7.1.74 | Ni2+ | about 20% as efficient as Mg2+ | Bos taurus | |
2.7.1.74 | Sr2+ | activation | Bos taurus | |
2.7.1.74 | Sr2+ | about 15% as efficient as Mg2+ | Bos taurus | |
2.7.1.74 | Zn2+ | activation | Bos taurus | |
2.7.1.74 | Zn2+ | about 40% as efficient as Mg2+ | Bos taurus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.74 | 56000 | - |
gel filtration, equilibrium density and sucrose density gradient centrifugation | Bos taurus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.74 | Bos taurus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.74 | - |
Bos taurus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.1.74 | thymus | - |
Bos taurus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.74 | 0.012 | - |
37°C, pH 8.0 | Bos taurus |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.1.74 | -30°C, several months in the presence of 2-mercaptoethanol | Bos taurus |
2.7.1.74 | 4°C, if purified in the absence of 2-mercaptoethanol it loses most of its activity within 2 days, DTT restores | Bos taurus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.74 | ATP + 2'-deoxycytidine | in the presence of DTT less effective than dUTP, UTP, dTTP or GTP, without DTT only less effective than dUTP | Bos taurus | ADP + 5'-dCMP | - |
? | |
2.7.1.74 | dTTP + 2'-deoxycytidine | - |
Bos taurus | dTDP + 2'-deoxy-CMP | - |
? | |
2.7.1.74 | dUTP + 2'-deoxycytidine | best phosphate donor | Bos taurus | dUDP + 2'-deoxy-CMP | - |
? | |
2.7.1.74 | GTP + 2'-deoxycytidine | - |
Bos taurus | GDP + 2'-deoxy-CMP | - |
? | |
2.7.1.74 | NTP + cytosine arabinoside | - |
Bos taurus | NDP + cytosine arabinoside 5'-monophosphate | - |
? | |
2.7.1.74 | NTP + deoxyadenosine | - |
Bos taurus | NDP + dAMP | - |
? | |
2.7.1.74 | NTP + deoxycytidine | - |
Bos taurus | NDP + dCMP | - |
? | |
2.7.1.74 | NTP + deoxyguanosine | - |
Bos taurus | NDP + dGMP | - |
? | |
2.7.1.74 | UTP + 2'-deoxycytidine | - |
Bos taurus | UDP + 2'-deoxy-CMP | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.74 | 37 | - |
assay at | Bos taurus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.74 | 22 | - |
at least 24 h, in the presence of dithiothreitol | Bos taurus |
2.7.1.74 | 60 | - |
dCTP enhances heat stability at pH 6, dithiothreitol slightly, not deoxycytidine or other nucleosides | Bos taurus |
2.7.1.74 | 60 | - |
t1/2: 7.9 min at pH 8, t1/2: 2 min at pH 6, unstable at pH 10 | Bos taurus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.74 | 6 | 10 | broad plateau, with very slight optima at pH 6 and 10 | Bos taurus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.74 | 6 | - |
t1/2: 2 min at 60°C | Bos taurus |
2.7.1.74 | 8 | - |
moderately stable, t1/2: 7.9 min at 60°C | Bos taurus |
2.7.1.74 | 10 | - |
unstable at 60°C | Bos taurus |