Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kessel, D.
    Properties of deoxycytidine kinase partially purified from L1210 cells (1968), J. Biol. Chem., 243, 4739-4744.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.74 bovine serum albumin does not stabilize Mus musculus
2.7.1.74 protamine treatment, MnCl2-treatment or ammonium sulfate fractionation inactivates during purification Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.74 cytidine not Mus musculus
2.7.1.74 cytosine arabinoside deoxycytidine as substrate Mus musculus
2.7.1.74 dAMP not Mus musculus
2.7.1.74 dCDP
-
 Mus musculus
2.7.1.74 dCMP
-
 Mus musculus
2.7.1.74 dCTP strong, pH-dependent Mus musculus
2.7.1.74 dGMP not Mus musculus
2.7.1.74 dinitrophenol weak Mus musculus
2.7.1.74 dioxane
-
 Mus musculus
2.7.1.74 dUMP not Mus musculus
2.7.1.74 HgCl2
-
 Mus musculus
2.7.1.74 IAA weak Mus musculus
2.7.1.74 additional information little or no inhibition by deoxyuridine; no inhibition by TTP; no inhibition by uridine, thymidine; relatively insensitive to SH-inhibitors and detergents Mus musculus
2.7.1.74 N-ethylmaleimide weak Mus musculus
2.7.1.74 SDS
-
 Mus musculus
2.7.1.74 Urea
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.74 additional information
-
 additional information kinetic study Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.74 cytosol
-
 Mus musculus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.74 Ca2+ activation Mus musculus
2.7.1.74 Ca2+ can replace Mn2+ or Mg2+ to some extent Mus musculus
2.7.1.74 Fe2+ activation Mus musculus
2.7.1.74 Fe2+ can replace Mn2+ or Mg2+ to some extent Mus musculus
2.7.1.74 Mg2+ requirement, 2 mM Mus musculus
2.7.1.74 Mn2+ requirement Mus musculus
2.7.1.74 Mn2+ 0.5 mM Mus musculus
2.7.1.74 additional information no activation by Ba2+, Cu2+ Mus musculus
2.7.1.74 Zn2+ not Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.74 60000
-
 gel filtration Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.74 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.74 partial Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.74 leukemia cell L1210 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.74 0.0048
-
 37°C, pH 7.0 Mus musculus

Storage Stability

EC Number Storage Stability Organism
2.7.1.74 -20°C, 3 months Mus musculus
2.7.1.74 4°C, at least 1 week Mus musculus
2.7.1.74 glycerol, bovine serum albumin, 2-mercaptoethanol or EDTA does not enhance storage stability Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.74 ATP + 2'-deoxycytidine best phosphate donor Mus musculus ADP + 5'-dCMP
-
 ?
2.7.1.74 NTP + cytosine arabinoside
-
 Mus musculus NDP + cytosine arabinoside 5'-monophosphate
-
 ?
2.7.1.74 NTP + deoxycytidine no substrate: phosphate Mus musculus NDP + dCMP
-
 ?
2.7.1.74 NTP + deoxycytidine enzyme shows little specificity towards phosphate donor Mus musculus NDP + dCMP
-
 ?
2.7.1.74 UTP + 2'-deoxycytidine best phosphate donor at low substrate levels Mus musculus UDP + 2'-deoxy-CMP
-
 ?
2.7.1.74 UTP + 2'-deoxycytidine as good as CTP, GTP or dATP Mus musculus UDP + 2'-deoxy-CMP
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.74 37
-
 assay at Mus musculus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.74 27 37 moderately sensitive to variations of incubation temperature Mus musculus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.74 50
-
 10 min, 10% loss of activity Mus musculus
2.7.1.74 60
-
 10 min, about 65% loss of activity Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.74 5 7 broad Mus musculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.74 5 10 maximal activity at pH 5 and about half-maximal activity at pH 10 Mus musculus