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Literature summary extracted from

  • Mori, H.; Iida, A.; Teshiba, S.; Fujio, T.
    Cloning of a guanosine-inosine kinase gene of Escherichia coli and characterization of the purified gene product (1995), J. Bacteriol., 177, 4921-4926.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.73 K+ the enzyme requires both Mg2+ and K+ Escherichia coli
2.7.1.73 Mg2+ enzyme requires both Mg2+ and K+ Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.73
-
 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.73 Cu2+
-
 Escherichia coli
2.7.1.73 Zn2+
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.73 0.0061
-
 guanosine pH 7.2, 30°C Escherichia coli
2.7.1.73 0.51
-
 ATP pH 7.2, 30°C, guanosine kinase reaction Escherichia coli
2.7.1.73 0.66
-
 dATP pH 7.2, 30°C, inosine kinase reaction Escherichia coli
2.7.1.73 0.71
-
 ATP pH 7.2, 30°C, inosine kinase reaction Escherichia coli
2.7.1.73 2.1
-
 Inosine pH 7.2, 30°C Escherichia coli
2.7.1.73 2.4
-
 dATP pH 7.2, 30°C, guanosine kinase reaction Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.73 48400
-
 x * 48400, calculation from nucleotide sequence Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.73 Escherichia coli P0AEW6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.73 recombinant enzyme Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.73 3.7
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.73 ATP + guanosine
-
 Escherichia coli ADP + GMP
-
 ?
2.7.1.73 ATP + inosine about 60% of the activity with guanosine Escherichia coli ADP + 5'-IMP
-
 ?
2.7.1.73 dATP + guanosine
-
 Escherichia coli dADP + GMP
-
 ?
2.7.1.73 dATP + inosine
-
 Escherichia coli dADP + IMP
-
 ?
2.7.1.73 UTP + guanosine UTP shows 20% of the activity with ATP Escherichia coli UDP + GMP
-
 ?
2.7.1.73 UTP + inosine UTP shows 20% of the activity with ATP Escherichia coli UDP + IMP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.73 ? x * 48400, calculation from nucleotide sequence Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.73 26 39 inosine kinase Escherichia coli
2.7.1.73 38
-
 guanosine kinase Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.73 6.9
-
 inosine kinase Escherichia coli
2.7.1.73 8.2
-
 guanosine kinase Escherichia coli