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Literature summary extracted from

  • Schmidt, C.L.; Daneel, H.J.; Schultz, G.; Buchanan, B.B.
    Shikimate kinase from spinach chloroplasts. Purification, characterization and regulatory function in aromatic amino acid biosynthesis (1990), Plant Physiol., 93, 758-766.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.71 ADP 0.5 mM; 12% inhibition Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.71 additional information
-
 additional information
-
 Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.71 chloroplast
-
 Spinacia oleracea 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.71 Mg2+ divalent cation required, Mg2+ is most effective followed by Mn2+. Optimal Mg2+ concentration is 2.4 mM Spinacia oleracea
2.7.1.71 Mn2+ divalent cation required, Mg2+ is most effective followed by Mn2+ Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.71 28000
-
 gel filtration Spinacia oleracea
2.7.1.71 31000
-
 1 * 31000, SDS-PAGE Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.71 ATP + shikimate Spinacia oleracea energy charge plays a role in regulating shikimate kinase, thereby controlling the shikimate pathway ADP + shikimate 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.71 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.71
-
 Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.71 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.71 8.39
-
-
 Spinacia oleracea

Storage Stability

EC Number Storage Stability Organism
2.7.1.71 -20°C, in presence of 15% glycerol, stable for up to 4 weeks without loss of activity Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.71 2'-deoxyATP + shikimate
-
 Spinacia oleracea 2'-deoxyADP + shikimate 3-phosphate
-
 ?
2.7.1.71 ATP + shikimate
-
 Spinacia oleracea ADP + shikimate 3-phosphate
-
 ?
2.7.1.71 ATP + shikimate energy charge plays a role in regulating shikimate kinase, thereby controlling the shikimate pathway Spinacia oleracea ADP + shikimate 3-phosphate
-
 ?
2.7.1.71 additional information no activity with XTP, ITP, GTP, TTP, CTP and UTP Spinacia oleracea ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.71 monomer 1 * 31000, SDS-PAGE Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.71 9.5
-
-
 Spinacia oleracea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.71 6 9.5 pH 6.0: about 50% of maximal activity, pH 9.5: optimum Spinacia oleracea