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Literature summary extracted from
- Purification of the mannitol-specific enzyme II of the Escherichia coli phosphoenolpyruvate:sugar phosphotransferase system (1979), J. Biol. Chem., 254, 249-252.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.7.1.197 | N-ethylmaleimide | rapid inactivation with 0.5 mM | Escherichia coli |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 2.7.1.197 | membrane | - |
Escherichia coli | 16020 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.7.1.197 | 60000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.1.197 | [protein HPr]-Npi-phospho-L-histidine + D-mannitol[side 1] | Escherichia coli | - |
[protein HPr]-L-histidine + D-mannitol 1-phosphate[side 2] | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.7.1.197 | Escherichia coli | P00550 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.7.1.197 | hexyl agarose column chromatography, omega-aminohexyl agarose column chromatography, and DEAE cellulose column chromatography | Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.7.1.197 | 0.023 | - |
unpurified enzyme from membrane, at pH 9.1 and 37°C | Escherichia coli |
| 2.7.1.197 | 26.67 | - |
after 235fold purification, at pH 9.1 and 37°C | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.1.197 | [protein HPr]-Npi-phospho-L-histidine + D-mannitol[side 1] | - |
Escherichia coli | [protein HPr]-L-histidine + D-mannitol 1-phosphate[side 2] | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.7.1.197 | mannitol-specific enzyme II | - |
Escherichia coli |
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