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Literature summary extracted from

  • Jacobson, G.R.; Lee, C.A.; Leonard, J.E.; Saier, M.H.
    Mannitol-specific enzyme II of the bacterial phosphotransferase system. I. Properties of the purified permease (1983), J. Biol. Chem., 258, 10748-10756.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.197 additional information the purified enzyme is dependent on lubrol PX and phospholipid for maximal activity Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.197 D-fructose 1,6-bisphosphate 34% inhibition at 20 mM Escherichia coli
2.7.1.197 D-glucitol
-
 Escherichia coli
2.7.1.197 D-glucose 1-phosphate
-
 Escherichia coli
2.7.1.197 D-glucose 6-phosphate
-
 Escherichia coli
2.7.1.197 D-mannitol strong substrate inhibition at pH 7.0 Escherichia coli
2.7.1.197 D-mannitol 1-phosphate strong substrate inhibition at pH 7.0 Escherichia coli
2.7.1.197 Trypsin
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.197 0.011
-
 D-mannitol[side 1] at pH 5.5 and 30°C Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.197 membrane integral membrane protein Escherichia coli 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.197 60000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.197 [protein]-L-histidine + D-mannitol 1-phosphate[side 2] Escherichia coli
-
 [protein]-Npi-phospho-L-histidine + D-mannitol[side 1]
-
 r
2.7.1.197 [protein]-Npi-phospho-L-histidine + D-mannitol[side 1] Escherichia coli
-
 [protein]-L-histidine + D-mannitol 1-phosphate[side 2]
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.197 Escherichia coli P00550
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.197 DEAE-cellulose column chromatography and hexyl agarose column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.197 additional information the purified enzyme does not catalyze hydrolysis of D-mannitol 1-phosphate Escherichia coli ?
-
 ?
2.7.1.197 [protein]-L-histidine + D-mannitol 1-phosphate[side 2]
-
 Escherichia coli [protein]-Npi-phospho-L-histidine + D-mannitol[side 1]
-
 r
2.7.1.197 [protein]-Npi-phospho-L-histidine + D-mannitol[side 1]
-
 Escherichia coli [protein]-L-histidine + D-mannitol 1-phosphate[side 2]
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.7.1.197 enzyme IImtl
-
 Escherichia coli
2.7.1.197 mannitol-specific enzyme II
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.197 6.5
-
-
 Escherichia coli