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Literature summary extracted from

  • Vancurova, I.; Choi, J.H.; Lin, H.; Kuret, J.; Vancura, A.
    Regulation of phosphatidylinositol 4-phosphate 5-kinase from Schizosaccharomyces pombe by casein kinase I (1999), J. Biol. Chem., 274, 1147-1155.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.68 spermine 0.5 mM, 300% increase in activity Schizosaccharomyces pombe

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.68 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate 0.1 mM, 55% inhibition Schizosaccharomyces pombe
2.7.1.68 phosphatidic acid 0.1 mM, 125% stimulation Schizosaccharomyces pombe
2.7.1.68 phosphatidylinositol 0.1 mM, 125% stimulation Schizosaccharomyces pombe
2.7.1.68 phosphatidylserine 0.1 mM, 125% stimulation Schizosaccharomyces pombe
2.7.1.68 spermine 0.03 mg/ml, 50% inhibition Schizosaccharomyces pombe

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.68 additional information
-
 additional information Km for 1-phosphatidyl-1D-myo-inositol 4-phosphate is 1.0 mol% Schizosaccharomyces pombe
2.7.1.68 0.008
-
 ATP pH 7.5 Schizosaccharomyces pombe

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.68 plasma membrane
-
 Schizosaccharomyces pombe 5886
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.68 63000
-
 x * 63000, SDS-PAGE Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.68 ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate Schizosaccharomyces pombe the enzyme catalyzes the last step in the synthesis of phosphatidylinositol 4,5-bisphosphate, which is a precursor of diacylglycerol and inositol 1,4,5-triphosphate and is also involved in regulation of actin cytoskeleton remodeling and membrane traffic. The enzyme is regulated by casein kinase Cki1 ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.68 Schizosaccharomyces pombe
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.68 phosphoprotein phosphorylation by Cki1 in vitro decreases the activity of the enzyme Schizosaccharomyces pombe

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.68
-
 Schizosaccharomyces pombe

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.68 1.704
-
-
 Schizosaccharomyces pombe

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.68 ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 Schizosaccharomyces pombe ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?
2.7.1.68 ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate the enzyme catalyzes the last step in the synthesis of phosphatidylinositol 4,5-bisphosphate, which is a precursor of diacylglycerol and inositol 1,4,5-triphosphate and is also involved in regulation of actin cytoskeleton remodeling and membrane traffic. The enzyme is regulated by casein kinase Cki1 Schizosaccharomyces pombe ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.68 ? x * 63000, SDS-PAGE Schizosaccharomyces pombe

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.68 7.5
-
-
 Schizosaccharomyces pombe

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.68 5.5 8.5 pH 5.5: about 35% of maximal activity, pH 8.5: about 60% of maximal activity Schizosaccharomyces pombe