BRENDA - Enzyme Database

Regulation of phosphatidylinositol 4-phosphate 5-kinase from Schizosaccharomyces pombe by casein kinase I

Vancurova, I.; Choi, J.H.; Lin, H.; Kuret, J.; Vancura, A.; J. Biol. Chem. 274, 1147-1155 (1999)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.68
spermine
0.5 mM, 300% increase in activity
Schizosaccharomyces pombe
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.68
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
0.1 mM, 55% inhibition
Schizosaccharomyces pombe
2.7.1.68
phosphatidic acid
0.1 mM, 125% stimulation
Schizosaccharomyces pombe
2.7.1.68
phosphatidylinositol
0.1 mM, 125% stimulation
Schizosaccharomyces pombe
2.7.1.68
phosphatidylserine
0.1 mM, 125% stimulation
Schizosaccharomyces pombe
2.7.1.68
spermine
0.03 mg/ml, 50% inhibition
Schizosaccharomyces pombe
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.68
additional information
-
additional information
Km for 1-phosphatidyl-1D-myo-inositol 4-phosphate is 1.0 mol%
Schizosaccharomyces pombe
2.7.1.68
0.008
-
ATP
pH 7.5
Schizosaccharomyces pombe
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.68
plasma membrane
-
Schizosaccharomyces pombe
5886
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.68
63000
-
x * 63000, SDS-PAGE
Schizosaccharomyces pombe
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
Schizosaccharomyces pombe
the enzyme catalyzes the last step in the synthesis of phosphatidylinositol 4,5-bisphosphate, which is a precursor of diacylglycerol and inositol 1,4,5-triphosphate and is also involved in regulation of actin cytoskeleton remodeling and membrane traffic. The enzyme is regulated by casein kinase Cki1
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.68
Schizosaccharomyces pombe
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
2.7.1.68
phosphoprotein
phosphorylation by Cki1 in vitro decreases the activity of the enzyme
Schizosaccharomyces pombe
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.68
-
Schizosaccharomyces pombe
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.68
1.704
-
-
Schizosaccharomyces pombe
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
641844
Schizosaccharomyces pombe
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
-
?
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
the enzyme catalyzes the last step in the synthesis of phosphatidylinositol 4,5-bisphosphate, which is a precursor of diacylglycerol and inositol 1,4,5-triphosphate and is also involved in regulation of actin cytoskeleton remodeling and membrane traffic. The enzyme is regulated by casein kinase Cki1
641844
Schizosaccharomyces pombe
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.1.68
?
x * 63000, SDS-PAGE
Schizosaccharomyces pombe
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.68
7.5
-
-
Schizosaccharomyces pombe
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.68
5.5
8.5
pH 5.5: about 35% of maximal activity, pH 8.5: about 60% of maximal activity
Schizosaccharomyces pombe
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.68
spermine
0.5 mM, 300% increase in activity
Schizosaccharomyces pombe
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.68
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
0.1 mM, 55% inhibition
Schizosaccharomyces pombe
2.7.1.68
phosphatidic acid
0.1 mM, 125% stimulation
Schizosaccharomyces pombe
2.7.1.68
phosphatidylinositol
0.1 mM, 125% stimulation
Schizosaccharomyces pombe
2.7.1.68
phosphatidylserine
0.1 mM, 125% stimulation
Schizosaccharomyces pombe
2.7.1.68
spermine
0.03 mg/ml, 50% inhibition
Schizosaccharomyces pombe
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.68
additional information
-
additional information
Km for 1-phosphatidyl-1D-myo-inositol 4-phosphate is 1.0 mol%
Schizosaccharomyces pombe
2.7.1.68
0.008
-
ATP
pH 7.5
Schizosaccharomyces pombe
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.68
plasma membrane
-
Schizosaccharomyces pombe
5886
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.68
63000
-
x * 63000, SDS-PAGE
Schizosaccharomyces pombe
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
Schizosaccharomyces pombe
the enzyme catalyzes the last step in the synthesis of phosphatidylinositol 4,5-bisphosphate, which is a precursor of diacylglycerol and inositol 1,4,5-triphosphate and is also involved in regulation of actin cytoskeleton remodeling and membrane traffic. The enzyme is regulated by casein kinase Cki1
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
?
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
2.7.1.68
phosphoprotein
phosphorylation by Cki1 in vitro decreases the activity of the enzyme
Schizosaccharomyces pombe
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.68
-
Schizosaccharomyces pombe
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.68
1.704
-
-
Schizosaccharomyces pombe
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
641844
Schizosaccharomyces pombe
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
-
?
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
the enzyme catalyzes the last step in the synthesis of phosphatidylinositol 4,5-bisphosphate, which is a precursor of diacylglycerol and inositol 1,4,5-triphosphate and is also involved in regulation of actin cytoskeleton remodeling and membrane traffic. The enzyme is regulated by casein kinase Cki1
641844
Schizosaccharomyces pombe
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.1.68
?
x * 63000, SDS-PAGE
Schizosaccharomyces pombe
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.68
7.5
-
-
Schizosaccharomyces pombe
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.68
5.5
8.5
pH 5.5: about 35% of maximal activity, pH 8.5: about 60% of maximal activity
Schizosaccharomyces pombe