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Literature summary extracted from

  • Cochet, C.; Chambaz, E.M.
    Catalytic properties of a purified phosphatidylinositol-4-phosphate kinase from rat brain (1986), Biochem. J., 237, 25-31.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.68 spermine strong activation in presence of micromolar concentrations, at 2 mM Mg2+ optimal spermine concentration is 0.1-0.2 mM spermine Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.68 heparin IC50: 0.002 mg/ml, competitive towards 1-phosphatidyl-1D-myo-inositol 4-phosphate Rattus norvegicus
2.7.1.68 quercetin IC50: 0.0002 mM, competitive towards ATP or GTP Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.68 additional information
-
 additional information Km-value for 1-phosphatidyl-1D-myo-inositol 4-phosphate is 0.0033 mg/ml Rattus norvegicus
2.7.1.68 0.025
-
 ATP pH 7.5, 22°C Rattus norvegicus
2.7.1.68 0.133
-
 GTP pH 7.5, 22°C Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.68 Mg2+ required, optimal concentration is 20-30 mM Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.68 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.68 partial Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.68 brain
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.68 1
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.7.1.68 -80°C, stable for at least 3 months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.68 ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 Rattus norvegicus ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?
2.7.1.68 GTP + 1-phosphatidyl-1D-myo-inositol 4-phosphate 50% of the activity with ATP Rattus norvegicus GDP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?