Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Okpodu, C.M.; Gross, W.; Burkhart, W.; Boss, W.F.
    Purification and Characterization of a Soluble Phosphatidylinositol 4-Kinase from Carrot Suspension Culture Cells (1995), Plant Physiol., 107, 491-500.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.67 Triton X-100 maximal activity occurs when the substrate is added as Triton X-100/phosphatidylinositol mixed micelles Daucus carota

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.67 adenosine 0.1 mM, no effect Daucus carota
2.7.1.67 ADP up to 0.1 mM, no effect Daucus carota
2.7.1.67 Ca2+ 0.01 mM to 1 mM: no effect Daucus carota

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.67 0.4
-
 ATP pH 8.0, 25°C Daucus carota

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.67 Ca2+ 0.01-1 mM, no effect Daucus carota
2.7.1.67 Mg2+ divalent cation required. At 1-5 mM, Mn2+ is more effective than Mg2+ in increasing enzyme activity. Maximal activity occurs at 25-40 mM Daucus carota
2.7.1.67 Mn2+ divalent cation required. At 1-5 mM, Mn2+ is more effective than Mg2+ in increasing enzyme activity Daucus carota

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.67 80000
-
 1 * 80000, SDS-PAGE Daucus carota
2.7.1.67 83000
-
 gel filtration Daucus carota

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.67 Daucus carota
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.67
-
 Daucus carota

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.67 cell suspension culture
-
 Daucus carota
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.67 0.0075
-
-
 Daucus carota

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.67 ATP + 1-phosphatidyl-1D-myo-inositol
-
 Daucus carota ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.67 monomer 1 * 80000, SDS-PAGE Daucus carota

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.67 8 8.5
-
 Daucus carota

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.67 6.5 8.5 pH 6.5: about 40% of maximal activity, pH 8.0-8.5: optimium Daucus carota