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Literature summary extracted from

  • Suer, S.; Sickmann, A.; Meyer, H.E.; Herberg, F.W.; Heilmeyer, L.M., Jr.
    Human phosphatidylinositol 4-kinase isoform PI4K92. Expression of the recombinant enzyme and determination of multiple phosphorylation sites (2001), Eur. J. Biochem., 268, 2099-2106.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.67 Triton X-100 optimal concentration is 0.1% Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.67 isoform PI4K92 is expressed as His6 tagged protein in Sf9 cells reaching a level of approximately 5% of cellular proteins Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.67 adenosine
-
 Homo sapiens
2.7.1.67 Wortmannin IC50: about 300 nM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.67 1
-
 ATP 37°C Homo sapiens
2.7.1.67 1
-
 phosphatidylinositol 37°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.67 Homo sapiens Q9UBF8
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.67 phosphoprotein phosphoprotein, additionally phosphate is incorporated by incubation with ATP/Mg or ATP/Mn. Phosphorylation sites are mapped by MALDI-MS and LC-MS/MS at the following positions: S258, T263, S266, S277, S294, T423, S496, T504 Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.67 type III kinase, isoform PI4K92 is expressed as His6 tagged protein in Sf9 cells Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.67 0.581
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.67 ATP + 1-phosphatidyl-1D-myo-inositol
-
 Homo sapiens ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.67 0.365
-
 adenosine 37°C Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.1.67 0.0003
-
 IC50: about 300 nM Homo sapiens Wortmannin