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Literature summary extracted from

  • Hou, W.M.; Zhang, Z.L.; Tai, H.H.
    Purification and characterization of phosphatidylinositol kinase from porcine liver microsomes (1988), Biochim. Biophys. Acta, 959, 67-75.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.67 adenosine IC50: 0.07 mM. 1 mM, 93% inhibition Sus scrofa
2.7.1.67 ADP IC50: 0.12 mM. 1 mM, 92% inhibition Sus scrofa
2.7.1.67 AMP 1 mM, 48% inhibition Sus scrofa
2.7.1.67 Ca2+ inhibits Mg2+-stimulated activity, IC50: 0.4 mM Sus scrofa
2.7.1.67 deoxycholate 10 mM, 35% inhibition Sus scrofa
2.7.1.67 GDP 1 mM, 27% inhibition Sus scrofa
2.7.1.67 GMP 1 mM, 20% inhibition Sus scrofa
2.7.1.67 GTP 1 mM, 24% inhibition Sus scrofa
2.7.1.67 guanosine 1 mM, 50% inhibition Sus scrofa
2.7.1.67 Lubrol Px 1%, 72% inhibition Sus scrofa
2.7.1.67 NEM 0.1 mM, 42% inhibition Sus scrofa
2.7.1.67 PCMB 0.1 mM, 98% inhibition Sus scrofa
2.7.1.67 phosphatidylethanolamine 0.1 mM, 24% inhibition Sus scrofa
2.7.1.67 phosphatidylglycerol 0.1 mM, 63% inhibition Sus scrofa
2.7.1.67 phosphatidylserine 0.1 mM, 33% inhibition Sus scrofa
2.7.1.67 quercetin IC50: 0.1 mM Sus scrofa
2.7.1.67 Sodium mersalyl 0.1 mM, 78% inhibition Sus scrofa
2.7.1.67 Triton X-100 0.2%, 11% inhibition Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.67 0.06
-
 ATP pH 7.5, 30°C Sus scrofa
2.7.1.67 0.12
-
 phosphatidylinositol pH 7.5, 30°C Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.67 microsome
-
 Sus scrofa
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.67 Co2+ divalent metal ions stimulate in decreasing order of efficiency: Mg2+, Fe2+, Mn2+, Fe3+, Co2+ Sus scrofa
2.7.1.67 Fe2+ divalent metal ions stimulate in decreasing order of efficiency: Mg2+, Fe2+, Mn2+, Fe3+, Co2+ Sus scrofa
2.7.1.67 Fe3+ divalent metal ions stimulate in decreasing order of efficiency: Mg2+, Fe2+, Mn2+, Fe3+, Co2+ Sus scrofa
2.7.1.67 Mg2+ divalent metal ions stimulate in decreasing order of efficiency: Mg2+, Fe2+, Mn2+, Fe3+, Co2+ Sus scrofa
2.7.1.67 Mn2+ divalent metal ions stimulate in decreasing order of efficiency: Mg2+, Fe2+, Mn2+, Fe3+, Co2+ Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.67 55000
-
 x * 55000, SDS-PAGE Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.67 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.67
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.67 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.67 8.824
-
-
 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.67 ATP + 1-phosphatidyl-1D-myo-inositol
-
 Sus scrofa ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.67 ? x * 55000, SDS-PAGE Sus scrofa

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.1.67 0.07
-
 IC50: 0.07 mM. 1 mM, 93% inhibition Sus scrofa adenosine
2.7.1.67 0.1
-
 IC50: 0.1 mM Sus scrofa quercetin
2.7.1.67 0.12
-
 IC50: 0.12 mM. 1 mM, 92% inhibition Sus scrofa ADP
2.7.1.67 0.4
-
 inhibits Mg2+-stimulated activity, IC50: 0.4 mM Sus scrofa Ca2+