BRENDA - Enzyme Database

Identification of phosphatidylinositol kinase in rat liver lysosomal membranes

Collins, C.A.; Wells, W.W.; J. Biol. Chem. 258, 2130-2134 (1983)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.67
Triton X-100
0.4% stimulates 2fold
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.67
Ca2+
1 mM
Rattus norvegicus
2.7.1.67
La3+
0.2 mM, 78% inhibition
Rattus norvegicus
2.7.1.67
phosphatidic acid
slight
Rattus norvegicus
2.7.1.67
Triton X-100
above 0.4% w/v
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.67
0.13
-
ATP
pH 7.5, 30°C, in presence of at least 20 mM MgCl2
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.67
lysosomal membrane
-
Rattus norvegicus
5765
-
2.7.1.67
lysosome
membrane
Rattus norvegicus
5764
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.67
Mg2+
optimal activity in presence of at least 20 mM MgCl2
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.67
Rattus norvegicus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.67
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.67
ATP + 1-phosphatidyl-1D-myo-inositol
-
641789
Rattus norvegicus
ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.67
7
9
-
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.67
Triton X-100
0.4% stimulates 2fold
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.67
Ca2+
1 mM
Rattus norvegicus
2.7.1.67
La3+
0.2 mM, 78% inhibition
Rattus norvegicus
2.7.1.67
phosphatidic acid
slight
Rattus norvegicus
2.7.1.67
Triton X-100
above 0.4% w/v
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.67
0.13
-
ATP
pH 7.5, 30°C, in presence of at least 20 mM MgCl2
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.67
lysosomal membrane
-
Rattus norvegicus
5765
-
2.7.1.67
lysosome
membrane
Rattus norvegicus
5764
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.67
Mg2+
optimal activity in presence of at least 20 mM MgCl2
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.67
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.67
ATP + 1-phosphatidyl-1D-myo-inositol
-
641789
Rattus norvegicus
ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.67
7
9
-
Rattus norvegicus