Literature summary extracted from

Flanagan, C.A.; Thorner, J.
Purification and characterization of a soluble phosphatidylinositol 4-kinase from the yeast Saccharomyces cerevisiae (1992), J. Biol. Chem., 267, 24117-24125.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
2.7.1.67	Triton X-100	stimulates	Saccharomyces cerevisiae

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.7.1.67	adenosine	10 mM, 30% inhibition; no inhibition	Saccharomyces cerevisiae
2.7.1.67	ADP	5 mM, 90% inhibition	Saccharomyces cerevisiae
2.7.1.67	ATPgammaS	5 mM 90% inhibition	Saccharomyces cerevisiae
2.7.1.67	phosphate	20 mM, 50% inhibition	Saccharomyces cerevisiae

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.7.1.67	0.05	-	phosphatidylinositol	pH 7.5, 34°C	Saccharomyces cerevisiae
2.7.1.67	0.1	-	ATP	pH 7.5, 34°C	Saccharomyces cerevisiae

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
2.7.1.67	soluble	-	Saccharomyces cerevisiae	-	-

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.7.1.67	125000	-	x * 125000, SDS-PAGE	Saccharomyces cerevisiae

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.7.1.67	Saccharomyces cerevisiae	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.7.1.67	-	Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.7.1.67	4.3	-	-	Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.7.1.67	ATP + 1-phosphatidyl-1D-myo-inositol	-	Saccharomyces cerevisiae	ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate	-	?

Subunits

EC Number	Subunits	Comment	Organism
2.7.1.67	?	x * 125000, SDS-PAGE	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)