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Literature summary extracted from

  • Walker, D.H.; Dougherty, N.; Pike, L.J.
    Purification and characterization of a phosphatidylinositol kinase from A431 cells (1988), Biochemistry, 27, 6504-6511.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.67 adenosine IC50: 0.09 mM Homo sapiens
2.7.1.67 ADP
-
 Homo sapiens
2.7.1.67 AMP
-
 Homo sapiens
2.7.1.67 Ca2+ inhibits in the presence of Mg2+ Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.67 0.016
-
 1-phosphatidyl-1D-myo-inositol pH 7.2, 30°C Homo sapiens
2.7.1.67 0.074
-
 ATP
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.67 membrane
-
 Homo sapiens 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.67 Mg2+ less than 1 mM is required Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.67 additional information
-
 the enzyme binds substantial amounts of Triton X-100 and is actually present in detergent-containing solutions as a complex with a molecular weight of 120000 Da. It seems likely that the active species of the enzyme is a single 55000 Da polypeptide chain bound to essentially one Triton X-100 micelle Homo sapiens
2.7.1.67 55000
-
 1 * 55000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.67 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.67
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.67 A-431 cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.67 1.05
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.7.1.67 -20°C, buffer containing 30% glycerol and 2 mM DTT, minimal loss of activity after 2 weeks Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.67 ATP + 1-phosphatidyl-1D-myo-inositol
-
 Homo sapiens ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.67 monomer 1 * 55000, SDS-PAGE Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.67 0.1
-
 Ca2+ pH 7.2, 30°C Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.1.67 0.09
-
 IC50: 0.09 mM Homo sapiens adenosine