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Literature summary extracted from

  • Szymona, M.; Ostrowski, W.
    Inorganic polyphosphate glucokinase of Mycobacterium (1964), Biochim. Biophys. Acta, 85, 283-295.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.63 dialysis against distilled water for 10 h leads to decreased activity Mycolicibacterium phlei
2.7.1.63 KCl, 0.3 M, stabilizes Mycolicibacterium phlei

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.63 tripolyphosphate when present in excess together with Graham‘s salt at pH 7.2 Mycolicibacterium phlei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.63 0.175
-
(Phosphate)n pH 8.5, 37°C, in terms of acid-labile phosphate Mycolicibacterium phlei
2.7.1.63 0.28
-
D-glucose pH 8.5, 37°C Mycolicibacterium phlei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.63 Mg2+ activation Mycolicibacterium phlei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.63 113000
-
sucrose density gradient centrifugation Mycolicibacterium phlei

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.63 Mycolicibacterium phlei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.63 partial Mycolicibacterium phlei

Storage Stability

EC Number Storage Stability Organism
2.7.1.63 frozen, partially purified preparation, several weeks Mycolicibacterium phlei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.63 (phosphate)n + D-glucose no utilization of triphosphate or tetrapolyphosphate Mycolicibacterium phlei (phosphate)n-1 + D-glucose 6-phosphate
-
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Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.63 37
-
24 h, pH 4-5: precipitation and 80% of activity retained, above pH 5.5: inactivation within 24 h, 0.3 M KCl stabilizes Mycolicibacterium phlei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.63 8.5
-
-
Mycolicibacterium phlei

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.63 3.5
-
t1/2: 24 h at 3°C or at 37°C plus 0.3 M KCl Mycolicibacterium phlei
2.7.1.63 5 7 24 h stable at 3°C Mycolicibacterium phlei