Literature summary extracted from
Szymona, M.; Ostrowski, W.
Inorganic polyphosphate glucokinase of Mycobacterium (1964), Biochim. Biophys. Acta, 85, 283-295.
General Stability
EC Number |
General Stability |
Organism |
---|
2.7.1.63 |
dialysis against distilled water for 10 h leads to decreased activity |
Mycolicibacterium phlei |
2.7.1.63 |
KCl, 0.3 M, stabilizes |
Mycolicibacterium phlei |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.7.1.63 |
tripolyphosphate |
when present in excess together with Grahams salt at pH 7.2 |
Mycolicibacterium phlei |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
2.7.1.63 |
0.175 |
- |
(Phosphate)n |
pH 8.5, 37°C, in terms of acid-labile phosphate |
Mycolicibacterium phlei |
|
2.7.1.63 |
0.28 |
- |
D-glucose |
pH 8.5, 37°C |
Mycolicibacterium phlei |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
2.7.1.63 |
Mg2+ |
activation |
Mycolicibacterium phlei |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
2.7.1.63 |
113000 |
- |
sucrose density gradient centrifugation |
Mycolicibacterium phlei |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.7.1.63 |
Mycolicibacterium phlei |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.7.1.63 |
partial |
Mycolicibacterium phlei |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
2.7.1.63 |
frozen, partially purified preparation, several weeks |
Mycolicibacterium phlei |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.7.1.63 |
(phosphate)n + D-glucose |
no utilization of triphosphate or tetrapolyphosphate |
Mycolicibacterium phlei |
(phosphate)n-1 + D-glucose 6-phosphate |
- |
? |
|
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
2.7.1.63 |
37 |
- |
24 h, pH 4-5: precipitation and 80% of activity retained, above pH 5.5: inactivation within 24 h, 0.3 M KCl stabilizes |
Mycolicibacterium phlei |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
2.7.1.63 |
8.5 |
- |
- |
Mycolicibacterium phlei |
pH Stability
EC Number |
pH Stability |
pH Stability Maximum |
Comment |
Organism |
---|
2.7.1.63 |
3.5 |
- |
t1/2: 24 h at 3°C or at 37°C plus 0.3 M KCl |
Mycolicibacterium phlei |
2.7.1.63 |
5 |
7 |
24 h stable at 3°C |
Mycolicibacterium phlei |