EC Number | General Stability | Organism |
---|---|---|
2.7.1.60 | purified enzyme is unstable to ammonium sulfate precipitation or freezing and thawing | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.60 | 0.2 | - |
N-acetyl-D-mannosamine | pH 8.1, 37°C | Rattus norvegicus | |
2.7.1.60 | 1.2 | - |
N-glycolylmannosamine | pH 8.1, 37°C | Rattus norvegicus | |
2.7.1.60 | 2 | - |
ATP | pH 8.1, 37°C | Rattus norvegicus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.60 | Mg2+ | required for activity | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.60 | ATP + N-acetyl-D-mannosamine | Rattus norvegicus | metabolic pathway between hexoses and sialic acids | ADP + N-acetyl-D-mannosamine 6-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.60 | Rattus norvegicus | - |
rat, albino | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.60 | - |
Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.1.60 | kidney | - |
Rattus norvegicus | - |
2.7.1.60 | liver | - |
Rattus norvegicus | - |
2.7.1.60 | additional information | no enzymatic activity in brain, heart and muscle | Rattus norvegicus | - |
2.7.1.60 | spleen | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.60 | 2.5 | - |
activity of fraction with N-glycolylmannosamine | Rattus norvegicus |
2.7.1.60 | 2.76 | - |
activity of fraction with N-glycolylmannosamine | Rattus norvegicus |
2.7.1.60 | 4 | - |
activity of fraction with N-acetylmannosamine | Rattus norvegicus |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.1.60 | 0°C, loses about 50% of its activity in 24 hours, stable for at least 1 week in presence of 0.01 M N-acylmannosamine | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.60 | ATP + N-acetyl-D-mannosamine | metabolic pathway between hexoses and sialic acids | Rattus norvegicus | ADP + N-acetyl-D-mannosamine 6-phosphate | - |
? | |
2.7.1.60 | ATP + N-acetyl-D-mannosamine | - |
Rattus norvegicus | ADP + N-acetyl-D-mannosamine-6-phosphate | - |
? | |
2.7.1.60 | ATP + N-glycolylmannosamine | - |
Rattus norvegicus | N-glycolylmannosamine-6-phosphate | - |
? | |
2.7.1.60 | additional information | N-acetylglucosamine and N-glycolylglucosamine, N-acetylgalactosamine, mannosamine, glucosamine, galactosamine, mannose, galactose, fructose, glucose, glucose-1-phosphate and glucose-6-phosphate are inactive as substrates, the phosphoryl donor ATP cannot be replaced by GTP, UTP, CTP, TTP, dATP or phosphoenolpyruvate | Rattus norvegicus | ? | - |
? |