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Literature summary extracted from

  • Timson, D.J.; Reece, R.J.
    Functional analysis of disease-causing mutations in human galactokinase (2003), Eur. J. Biochem., 270, 1767-1774.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.6 expression in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.6 A198V very similar kinetic parameters to the wild-type enzyme Homo sapiens
2.7.1.6 A384P mutant enzyme is not present in the soluble fraction after sonication and can not be purified Homo sapiens
2.7.1.6 C32M mutant enzyme is not present in the soluble fraction after sonication and can not be purified Homo sapiens
2.7.1.6 G346S mutant enzyme shows substantial reduction in turnover number. Lower specificity constant for galactose than wild-type enzyme Homo sapiens
2.7.1.6 G347S mutant enzyme shows substantial reduction in turnover number, increase in Km-value for galactose. Lower specificity constant for galactose than wild-type enzyme Homo sapiens
2.7.1.6 G349S lower specificity constant for galactose than wild-type enzyme. Lower specificity constant for galactose than wild-type enzyme Homo sapiens
2.7.1.6 G36R mutant enzyme is not present in the soluble fraction after sonication and can not be purified Homo sapiens
2.7.1.6 H44Y increrase in Km-value for galactose compared to wild-type. Lower specificity constant for galactose than wild-type enzyme Homo sapiens
2.7.1.6 P28T mutant enzyme is not present in the soluble fraction after sonication and can not be purified Homo sapiens
2.7.1.6 T288M mutant enzyme is not present in the soluble fraction after sonication and can not be purified Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.6 alpha-D-galactose 1-phosphate uncompetitive inhibitor with respect to galactose, mixed inhibitor with respect to ATP Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.6 0.005
-
 ATP pH 8, 37°C, mutant enzyme G346S Homo sapiens
2.7.1.6 0.026
-
 ATP pH 8, 37°C, mutant enzyme A198V Homo sapiens
2.7.1.6 0.034
-
 ATP pH 8, 37°C, wild-type enzyme Homo sapiens
2.7.1.6 0.039
-
 ATP pH 8, 37°C, mutant enzyme G349S Homo sapiens
2.7.1.6 0.089
-
 ATP pH 8, 37°C, mutant enzyme G347S Homo sapiens
2.7.1.6 0.11
-
 ATP pH 8, 37°C, mutant enzyme R68C Homo sapiens
2.7.1.6 0.13
-
 ATP pH 8, 37°C, mutant enzyme H44Y Homo sapiens
2.7.1.6 0.43
-
 D-galactose pH 8, 37°C, mutant enzyme R68C Homo sapiens
2.7.1.6 0.66
-
 D-galactose pH 8, 37°C, mutant enzyme A198V Homo sapiens
2.7.1.6 0.97
-
 D-galactose pH 8, 37°C, wild-type enzyme Homo sapiens
2.7.1.6 1.1
-
 D-galactose pH 8, 37°C, mutant enzyme G346S Homo sapiens
2.7.1.6 1.7
-
 D-galactose pH 8, 37°C, mutant enzyme G349S Homo sapiens
2.7.1.6 7.7
-
 D-galactose pH 8, 37°C, mutant enzyme H44Y Homo sapiens
2.7.1.6 13
-
 D-galactose pH 8, 37°C, mutant enzyme G347S Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.6 ATP + galactose Homo sapiens lack of fully functional galactokinase is one cause of the inherited disease galactosemia, the main clinical manifestation of which is the early onset of cataracts ADP + alpha-D-galactose 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.6 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.6
-
 Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.6 ATP + alpha-D-galactose = ADP + alpha-D-galactose 1-phosphate the most likely kinetic mechanism is an ordered ternary complex in which ATP is the first substrate to bind Homo sapiens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.6 ATP + D-galactose
-
 Homo sapiens ADP + alpha-D-galactose 1-phosphate
-
 ?
2.7.1.6 ATP + galactose lack of fully functional galactokinase is one cause of the inherited disease galactosemia, the main clinical manifestation of which is the early onset of cataracts Homo sapiens ADP + alpha-D-galactose 1-phosphate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.6 0.4
-
 ATP pH 8, 37°C, mutant enzyme G346S Homo sapiens
2.7.1.6 0.4
-
 D-galactose pH 8, 37°C, mutant enzyme G346S Homo sapiens
2.7.1.6 1.1
-
 ATP pH 8, 37°C, mutant enzyme G347S Homo sapiens
2.7.1.6 1.1
-
 D-galactose pH 8, 37°C, mutant enzyme G347S Homo sapiens
2.7.1.6 1.8
-
 ATP pH 8, 37°C, mutant enzyme G349S Homo sapiens
2.7.1.6 1.8
-
 D-galactose pH 8, 37°C, mutant enzyme G349S Homo sapiens
2.7.1.6 2
-
 ATP pH 8, 37°C, mutant enzyme H44Y Homo sapiens
2.7.1.6 2
-
 D-galactose pH 8, 37°C, mutant enzyme H44Y Homo sapiens
2.7.1.6 3.9
-
 ATP pH 8, 37°C, mutant enzyme R68C Homo sapiens
2.7.1.6 3.9
-
 D-galactose pH 8, 37°C, mutant enzyme R68C Homo sapiens
2.7.1.6 5.9
-
 ATP pH 8, 37°C, mutant enzyme R198V Homo sapiens
2.7.1.6 5.9
-
 D-galactose pH 8, 37°C, mutant enzyme R198V Homo sapiens
2.7.1.6 8.7
-
 ATP pH 8, 37°C, wild-type enzyme Homo sapiens
2.7.1.6 8.7
-
 D-galactose pH 8, 37°C, wild-type enzyme Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.6 28
-
 alpha-D-galactose 1-phosphate pH 8, 37°C, uncompetitive inhibition constant with respect to galactose Homo sapiens
2.7.1.6 39
-
 alpha-D-galactose 1-phosphate pH 8, 37°C, uncompetitive inhibition constant with respect to ATP Homo sapiens
2.7.1.6 90
-
 alpha-D-galactose 1-phosphate pH 8, 37°C, competitive inhibition constant with respect to ATP Homo sapiens