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Literature summary extracted from

  • Dey, P.M.
    Galactokinase of Vicia faba seeds (1983), Eur. J. Biochem., 136, 155-159.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.6 3-mercaptoethanol
-
 Vicia faba
2.7.1.6 ADP
-
 Vicia faba
2.7.1.6 alpha-D-galactose 1-phosphate
-
 Vicia faba
2.7.1.6 Cu2+ 1 mM, complete inhibition Vicia faba
2.7.1.6 D-galactose 1-phosphate
-
 Vicia faba
2.7.1.6 Hg2+ 1 mM, complete inhibition Vicia faba
2.7.1.6 iodoacetamide 0.05 mM, complete inactivation Vicia faba
2.7.1.6 NEM 0.05 mM, complete inactivation Vicia faba
2.7.1.6 PCMB 0.005 mM, complete inactivation Vicia faba
2.7.1.6 Zn2+ 1 mM, complete inhibition Vicia faba

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.6 0.5
-
 D-galactose pH 7.3, 25°C Vicia faba
2.7.1.6 1.2
-
 D-galactosamine pH 7.3, 25°C Vicia faba
2.7.1.6 1.5
-
 ATP pH 7.5, 25°C Vicia faba
2.7.1.6 2.2
-
 2-deoxy-D-galactose pH 7.5, 25°C Vicia faba

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.6 Ca2+ divalent cation required, 7% of the activation with Mg2+ Vicia faba
2.7.1.6 Co2+ divalent cation required, 70% of the activation with Mg2+ Vicia faba
2.7.1.6 Mg2+ divalent cation required, Mg2+ is the best activator Vicia faba
2.7.1.6 Mn2+ divalent cation required, 40% of the activation with Mg2+ Vicia faba
2.7.1.6 Ni2+ divalent cation required, 15% of the activation with Mg2+ Vicia faba

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.6 30900
-
 2 * 30900, SDS-PAGE Vicia faba
2.7.1.6 60000
-
 gel filtration Vicia faba

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.6 Vicia faba
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.6
-
 Vicia faba

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.6 seed
-
 Vicia faba
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.6 2.258
-
-
 Vicia faba

Storage Stability

EC Number Storage Stability Organism
2.7.1.6 -30°C, stable for 2 months with only 5-10% loss of activity Vicia faba

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.6 ATP + 2-deoxy-D-galactose
-
 Vicia faba ADP + 2-deoxy-D-galactose 1-phosphate
-
 ?
2.7.1.6 ATP + D-galactosamine
-
 Vicia faba ADP + D-galactosamine 1-phosphate
-
 ?
2.7.1.6 ATP + D-galactose
-
 Vicia faba ADP + alpha-D-galactose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.6 dimer 2 * 30900, SDS-PAGE Vicia faba

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.6 20 45 pH 7.3, 50 mM phosphate buffer, 10 mM 2-mercaptoethanol, 5% glycerol, 60 min, stable Vicia faba

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.6 7.3
-
-
 Vicia faba

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.6 4.5 6.5 25°C, 30 min, stable Vicia faba

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.6 3.5
-
 D-galactose 1-phosphate pH 7.3, 25°C, competitive inhibition with galactose as the variable substrate Vicia faba
2.7.1.6 4.8
-
 ADP pH 7.3, 25°C, non-competitive with respect to ATP Vicia faba
2.7.1.6 8.5
-
 ADP non-competitive with respect to galactose Vicia faba

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.6 Vicia faba isoelectric focusing
-
 5.3