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Literature summary extracted from

  • Hinderlich, S.; Nohring, S.; Weise, C.; Franke, P.; Stasche, R.; Reutter, W.
    Purification and characterization of N-acetylglucosamine kinase from rat liver. Comparison with UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (1998), Eur. J. Biochem., 252, 133-139.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.59 5,5'-dithiobis(2-nitrobenzoic acid) substrates partially protect against inactivation Rattus norvegicus
2.7.1.59 arsenite/2,3-dimercaptopropanol substrates partially protect against inactivation Rattus norvegicus
2.7.1.59 Diamide substrates partially protect against inactivation Rattus norvegicus
2.7.1.59 iodoacetamide substrates partially protect against inactivation Rattus norvegicus
2.7.1.59 N-ethylmaleimide substrates partially protect against inactivation Rattus norvegicus
2.7.1.59 Periodate substrates partially protect against inactivation Rattus norvegicus
2.7.1.60 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Rattus norvegicus
2.7.1.60 arsenite/2,3-dimercaptopropanol
-
 Rattus norvegicus
2.7.1.60 Diamide
-
 Rattus norvegicus
2.7.1.60 iodoacetamide
-
 Rattus norvegicus
2.7.1.60 N-ethylmaleimide
-
 Rattus norvegicus
2.7.1.60 Periodate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.59 0.05
-
 N-acetyl-D-glucosamine 37°C, pH 7.5 Rattus norvegicus
2.7.1.59 1
-
 N-acetyl-D-mannosamine 37°C, pH 7.5 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.59 cytoplasm
-
 Rattus norvegicus 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.59 39000
-
 identification of 90000 Da band, found to be another protein Rattus norvegicus
2.7.1.59 39000
-
 SDS-PAGE, second band at 90000 Da shows no activity Rattus norvegicus
2.7.1.59 80000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.59 ATP + N-acetyl-D-glucosamine Rattus norvegicus
-
 ADP + N-acetyl-D-glucosamine 6-phosphate
-
 ?
2.7.1.60 ATP + N-acetyl-D-mannosamine Rattus norvegicus involved in N-acetylneuraminic acid metabolism, key enzyme in N-acetylneuraminic acid biosynthesis ADP + N-acetyl-D-mannosamine 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.59 Rattus norvegicus
-
-
-
2.7.1.60 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.59
-
 Rattus norvegicus
2.7.1.60
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.59 liver
-
 Rattus norvegicus
-
2.7.1.60 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.59 52.4
-
 purified enzyme, 37°C, pH 7.5 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.59 ATP + N-acetyl-D-glucosamine
-
 Rattus norvegicus ADP + N-acetyl-D-glucosamine 6-phosphate
-
 ?
2.7.1.59 ATP + N-acetyl-D-mannosamine
-
 Rattus norvegicus ADP + N-acetyl-D-mannosamine 6-phosphate
-
 ?
2.7.1.60 ATP + N-acetyl-D-mannosamine involved in N-acetylneuraminic acid metabolism, key enzyme in N-acetylneuraminic acid biosynthesis Rattus norvegicus ADP + N-acetyl-D-mannosamine 6-phosphate
-
 ?
2.7.1.60 ATP + N-acetyl-D-mannosamine
-
 Rattus norvegicus ADP + N-acetyl-D-mannosamine-6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.59 dimer 2 * 39000, SDS-PAGE, gel filtration Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.60 EC 5.1.3.14/EC 2.7.1.60
-
 Rattus norvegicus
2.7.1.60 UDP-GlcNAc-epimerase/ManNAc kinase
-
 Rattus norvegicus