Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Datta, A.
    N-Acetyl-D-glucosamine kinase from hog spleen (1975), Methods Enzymol., 42c, 58-62.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.59 2- mercaptoethanol
-
 Sus scrofa
2.7.1.59 cysteine reverses inhibition with p-chloroxymercuribenzoate Sus scrofa

General Stability

EC Number General Stability Organism
2.7.1.59 unstable to freezing and thawing, heat and low pH Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.59 CTP
-
 Sus scrofa
2.7.1.59 diphosphate
-
 Sus scrofa
2.7.1.59 N-acetyl-D-glucosamine-6-phosphate non-competitive Sus scrofa
2.7.1.59 p-chloromercuribenzoate reversible with cysteine or 2-mercaptoethanol Sus scrofa
2.7.1.59 phosphate
-
 Sus scrofa
2.7.1.59 UDP-N-acetylglucosamine
-
 Sus scrofa
2.7.1.59 UTP
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.59 1.1
-
 N-acetyl-D-glucosamine 37°C, pH 9.0 Sus scrofa
2.7.1.59 1.8
-
 ATP 37°C, pH 9.0 Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.59 Co2+ can partially replace Mg2+ in activation Sus scrofa
2.7.1.59 Mg2+ required Sus scrofa
2.7.1.59 Mn2+ can partially replace Mg2+ in activation Sus scrofa
2.7.1.59 additional information no activity with Ca2+, Ba2+ Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.59 ATP + N-acetyl-D-glucosamine Sus scrofa involved in UDP-N-acetylglucosamine formation ADP + N-acetyl-D-glucosamine 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.59 Sus scrofa
-
 hog
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.59
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.59 spleen
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.59 90
-
 purified enzyme, 37°C, pH 9.5 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
2.7.1.59 4°C, 0.02 M potassium phosphate buffer, pH 7.6, 0.001 M EDTA, 0.01 M 2-mercaptoethanol, stable for at least a week Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.59 ATP + N-acetyl-D-glucosamine
-
 Sus scrofa ADP + N-acetyl-D-glucosamine 6-phosphate
-
 ?
2.7.1.59 ATP + N-acetyl-D-glucosamine involved in UDP-N-acetylglucosamine formation Sus scrofa ADP + N-acetyl-D-glucosamine 6-phosphate
-
 ?
2.7.1.59 GTP + N-acetyl-D-glucosamine 30% of the activity with ATP Sus scrofa GDP + N-acetyl-D-glucosamine 6-phosphate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.59 70
-
 50% loss of activity within 2 min Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.59 8.6 9.4
-
 Sus scrofa