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Literature summary extracted from

  • Gindzienski, A.; Glowacka, D.; Zwierz, K.
    Purification and properties of N-acetylglucosamine kinase from human gastric mucosa (1974), Eur. J. Biochem., 43, 155-160.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.59 unstable to dialysis and gel filtration, 0.013 mM substrate protects against inactivation Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.59 p-chloromercuribenzoate irreversible Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.59 0.11
-
N-acetyl-D-glucosamine 37°C, pH 8.0 Homo sapiens
2.7.1.59 1.8
-
ATP 37°C, pH 8.0 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.59 Co2+ can partially replace Mg2+ in activation Homo sapiens
2.7.1.59 Mg2+ required Homo sapiens
2.7.1.59 Mn2+ can partially replace Mg2+ in activation Homo sapiens
2.7.1.59 additional information slight activities with Ni2+, Zn2+, Cu2+ Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.59 77000
-
gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.59 ATP + N-acetyl-D-glucosamine Homo sapiens part of glycoprotein synthesis pathway ADP + N-acetyl-D-glucosamine 6-phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.59 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.59
-
Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.59 gastric mucosa
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.59 1.28
-
purified enzyme, 37°C, pH 8.0 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.7.1.59 -20°C, stable for at least 6 months Homo sapiens
2.7.1.59 4°C, stable for at least 3 days Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.59 ATP + N-acetyl-D-glucosamine
-
Homo sapiens ADP + N-acetyl-D-glucosamine 6-phosphate
-
?
2.7.1.59 ATP + N-acetyl-D-glucosamine part of glycoprotein synthesis pathway Homo sapiens ADP + N-acetyl-D-glucosamine 6-phosphate
-
?
2.7.1.59 ATP + N-acetyl-D-mannosamine roughly 50% of activity Homo sapiens ADP + N-acetyl-D-mannosamine 6-phosphate
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.59 60
-
84% loss of activity within 2 min, no protection with added substrate Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.59 7.8 8
-
Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.59 5
-
50% loss of activity within 2 min, no protection with added substrate Homo sapiens