Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Asensio, C.; Ruiz-Amil, M.
    N-Acetyl-D-glucosamine kinase. II. Escherichia coli (1966), Methods Enzymol., 9, 421-425.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.59 ADP strong competitive inhibitor Escherichia coli
2.7.1.59 D-glucosamine
-
 Escherichia coli
2.7.1.59 N-Methyl-D-glucosamine
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.59 0.00005
-
 N-acetyl-D-glucosamine 37°C, pH 7.8 Escherichia coli
2.7.1.59 0.00014
-
 ATP 37°C, pH 7.8 Escherichia coli
2.7.1.59 0.00025
-
 ITP 37°C, pH 7.8 Escherichia coli
2.7.1.59 0.005
-
 D-glucose 37°C, pH 7.8 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.59 ATP + N-acetyl-D-glucosamine Escherichia coli
-
 ADP + N-acetyl-D-glucosamine 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.59 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.59
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.59 4.6
-
 purified enzyme, 37°C, pH 7.8 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.7.1.59 -20°C, stable for several months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.59 ATP + D-glucose 15% of the rate with N-acetyl-D-glucosamine Escherichia coli ADP + D-glucose 6-phosphate
-
 ?
2.7.1.59 ATP + N-acetyl-D-glucosamine
-
 Escherichia coli ADP + N-acetyl-D-glucosamine 6-phosphate
-
 ?
2.7.1.59 ITP + N-acetyl-D-glucosamine 75% of the activity with ATP Escherichia coli IDP + N-acetyl-D-glucosamine 6-phosphate
-
 ?
2.7.1.59 additional information N-methyl-D-glucosamine, D-glucosamine, N-acetyl-D-galactosamine, N-acetyl-D-mannosamine do not serve as substrates, very low activities with UTP, GTP and CTP Escherichia coli ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.59 65
-
 heat treatment for 5 min included in purification procedure Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.59 7.5 9.5
-
 Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.59 9.5
-
 no loss of activity within 30 min at 30°C Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.59 0.001
-
 N-Methyl-D-glucosamine 37°C, pH 7.8 Escherichia coli
2.7.1.59 0.01
-
 D-glucosamine 37°C, pH 7.8 Escherichia coli