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Literature summary extracted from

  • Bang, S.S.; Baumann, P.; Sawyer, M.H.
    Properties of 1-phosphofructokinase from Pseudomonas putida (1977), Can. J. Microbiol., 23, 721-725.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.56 additional information no activation by pyruvate, phosphoenolpyruvate, AMP, cAMP, citrate, fructose 6-phosphate, glucose 6-phosphate, 6-phosphogluconate or 2-keto-3-deoxy-6-phosphogluconate Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.56 ADP 2 mM, 45% inhibition, 5 mM ATP completely relieves inhibition Pseudomonas putida
2.7.1.56 ATP at a Mg2+/ATP ratio below 2; free form Pseudomonas putida
2.7.1.56 additional information not inhibited by 6-phosphogluconate, 2-oxo-3-deoxy-6-phosphogluconate; not inhibited by cAMP, pyruvate, phosphoenolpyruvate; not inhibited by citrate; not inhibited by CTP and UTP; not inhibited by D-fructose 6-phosphate; not inhibited by D-glucose 6-phosphate; not inhibited by phosphate, AMP Pseudomonas putida
2.7.1.56 SO42-
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.56 0.16
-
 D-Fructose 1-phosphate pH 8.0, 25°C Pseudomonas putida
2.7.1.56 0.19
-
 D-Fructose 1-phosphate pH 9.0, 25°C Pseudomonas putida
2.7.1.56 0.41
-
 ATP pH 8.0, 25°C Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.56 K+ 30-50 mM, 2.3fold activation Pseudomonas putida
2.7.1.56 Mg2+ required for activity Pseudomonas putida
2.7.1.56 Mg2+ sigmoidal response, maximal activity at 5 mM Pseudomonas putida
2.7.1.56 Mg2+ actual substrate: MgATP2- Pseudomonas putida
2.7.1.56 additional information not activated by phosphate Pseudomonas putida
2.7.1.56 Na+ 30-50 mM, 1.5fold activation Pseudomonas putida
2.7.1.56 NH4+ 30-50 mM, 1.5fold activation Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.56 ATP + D-fructose 1-phosphate Pseudomonas putida sole pathway of D-fructose metabolism in several Pseudomonas species ADP + D-fructose 1,6-bisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.56 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.56 ammonium sulfate, DEAE-Sephadex, partially purified Pseudomonas putida

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.56 4.02
-
-
 Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
2.7.1.56 4°C, 0.8-1.3 mg protein/ml in 50 mM phosphate buffer, pH 7, 0.5 mM dithiothreitol, 5-10% loss of activity Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.56 ATP + D-fructose 1-phosphate
-
 Pseudomonas putida ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.56 ATP + D-fructose 1-phosphate sole pathway of D-fructose metabolism in several Pseudomonas species Pseudomonas putida ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.56 GTP + D-fructose 1-phosphate
-
 Pseudomonas putida GDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.56 ITP + D-fructose 1-phosphate
-
 Pseudomonas putida IDP + D-fructose 1,6-bisphosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.56 25
-
 assay at Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.56 9
-
-
 Pseudomonas putida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.56 5.5 10.2 very low activity at pH 5.5, approx. half-maximal activity at pH 6.3 and pH 10.2 Pseudomonas putida