EC Number | General Stability | Organism |
---|---|---|
2.7.1.56 | freeze-thawing, stable to | Clostridium pasteurianum |
2.7.1.56 | Tris-HCl buffer in purification and electrophoresis leads to partial denaturation and loss of activity, stable in phosphate buffer | Clostridium pasteurianum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.56 | ADP | competitive vs. ATP | Clostridium pasteurianum | |
2.7.1.56 | ATP | - |
Clostridium pasteurianum | |
2.7.1.56 | CTP | if concentration exceeds Mg2+ concentration | Clostridium pasteurianum | |
2.7.1.56 | Cu2+ | 0.5 mM, 50% inhibition in the presence of 3 mM Mg2+ | Clostridium pasteurianum | |
2.7.1.56 | D-fructose 1,6-bisphosphate | noncompetitive vs. fructose 1-phosphate | Clostridium pasteurianum | |
2.7.1.56 | GTP | - |
Clostridium pasteurianum | |
2.7.1.56 | Hg2+ | 0.002 mM, 50% inhibition in the presence of 3 mM Mg2+ | Clostridium pasteurianum | |
2.7.1.56 | ITP | - |
Clostridium pasteurianum | |
2.7.1.56 | additional information | not inhibited by cAMP, pyruvate, phosphoenolpyruvate; not inhibited by citrate; not inhibited by D-fructose 6-phosphate; not inhibited by D-glucose 1-phosphate; not inhibited by D-glucose 6-phosphate; not inhibited by monovalent cations, glucose, ADPglucose, UDPglucose, NAD(H), NADP(H), dihydroxyacetone phosphate, glyceraldehyde 3-phosphate, 3-phosphoglycerate, CoA, acetyl-CoA, acetoacetyl-CoA; not inhibited by phosphate, AMP | Clostridium pasteurianum | |
2.7.1.56 | Ni2+ | 3 mM, 50% inhibition in the presence of 3 mM Mg2+ | Clostridium pasteurianum | |
2.7.1.56 | SO42- | - |
Clostridium pasteurianum | |
2.7.1.56 | UTP | if concentration exceeds Mg2+ concentration | Clostridium pasteurianum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.56 | 0.14 | - |
ATP | pH 8.0, 25°C | Clostridium pasteurianum | |
2.7.1.56 | 0.62 | - |
D-Fructose 1-phosphate | pH 8.0, 25°C | Clostridium pasteurianum | |
2.7.1.56 | 0.67 | - |
ITP | pH 8.0, 25°C | Clostridium pasteurianum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.56 | Co2+ | 3 mM, 80% of activity with Mn2+ | Clostridium pasteurianum | |
2.7.1.56 | Fe2+ | 3 mM, 50% of activity with Mn2+ | Clostridium pasteurianum | |
2.7.1.56 | Mg2+ | required for activity | Clostridium pasteurianum | |
2.7.1.56 | Mg2+ | 3 mM, 84% of activity with Mn2+ | Clostridium pasteurianum | |
2.7.1.56 | Mn2+ | can replace Mg2+ | Clostridium pasteurianum | |
2.7.1.56 | Mn2+ | required for activity | Clostridium pasteurianum | |
2.7.1.56 | Zn2+ | 3 mM, 34% of activity with Mn2+ | Clostridium pasteurianum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.56 | 21400 | - |
1 * 21400 + 1 * 35500, SDS-PAGE, after treatment with 8 M urea and 2-mercaptoethanol | Clostridium pasteurianum |
2.7.1.56 | 28500 | - |
1 * 28500 + 1 * 40000, SDS-PAGE, after treatment with 2-mercaptoethanol | Clostridium pasteurianum |
2.7.1.56 | 35500 | - |
1 * 21400 + 1 * 35500, SDS-PAGE, after treatment with 8 M urea and 2-mercaptoethanol | Clostridium pasteurianum |
2.7.1.56 | 40000 | - |
1 * 28500 + 1 * 40000, SDS-PAGE, after treatment with 2-mercaptoethanol | Clostridium pasteurianum |
2.7.1.56 | 62500 | - |
low-speed sedimentation equilibrium | Clostridium pasteurianum |
2.7.1.56 | 63100 | - |
gel filtration | Clostridium pasteurianum |
2.7.1.56 | 63200 | - |
sucrose density gradient centrifugation | Clostridium pasteurianum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.56 | Clostridium pasteurianum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.56 | CM-Sephadex, calcium phosphate gel, Sephadex G-100, DEAE-Sephadex, hydroxyapatite | Clostridium pasteurianum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.56 | additional information | - |
- |
Clostridium pasteurianum |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.1.56 | -20°C, 50 mM phosphate buffer, pH 7.5, 1 year, no loss of activity | Clostridium pasteurianum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.56 | ATP + D-fructose 1-phosphate | - |
Clostridium pasteurianum | ADP + D-fructose 1,6-bisphosphate | - |
? | |
2.7.1.56 | ITP + D-fructose 1-phosphate | - |
Clostridium pasteurianum | IDP + D-fructose 1,6-bisphosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.56 | dimer | 1 * 21400 + 1 * 35500, SDS-PAGE, after treatment with 8 M urea and 2-mercaptoethanol | Clostridium pasteurianum |
2.7.1.56 | dimer | 1 * 28500 + 1 * 40000, SDS-PAGE, after treatment with 2-mercaptoethanol | Clostridium pasteurianum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.56 | 7.8 | 9.2 | - |
Clostridium pasteurianum |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.56 | 7 | 9.8 | approx. half-maximal activity at pH 7 and 9.8 | Clostridium pasteurianum |