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Literature summary extracted from

  • Van Hugo, H.; Gottschalk, G.
    Purification and properties of 1-phosphofructokinase from Clostridium pasteurianum (1974), Eur. J. Biochem., 48, 455-463.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.56 freeze-thawing, stable to Clostridium pasteurianum
2.7.1.56 Tris-HCl buffer in purification and electrophoresis leads to partial denaturation and loss of activity, stable in phosphate buffer Clostridium pasteurianum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.56 ADP competitive vs. ATP Clostridium pasteurianum
2.7.1.56 ATP
-
Clostridium pasteurianum
2.7.1.56 CTP if concentration exceeds Mg2+ concentration Clostridium pasteurianum
2.7.1.56 Cu2+ 0.5 mM, 50% inhibition in the presence of 3 mM Mg2+ Clostridium pasteurianum
2.7.1.56 D-fructose 1,6-bisphosphate noncompetitive vs. fructose 1-phosphate Clostridium pasteurianum
2.7.1.56 GTP
-
Clostridium pasteurianum
2.7.1.56 Hg2+ 0.002 mM, 50% inhibition in the presence of 3 mM Mg2+ Clostridium pasteurianum
2.7.1.56 ITP
-
Clostridium pasteurianum
2.7.1.56 additional information not inhibited by cAMP, pyruvate, phosphoenolpyruvate; not inhibited by citrate; not inhibited by D-fructose 6-phosphate; not inhibited by D-glucose 1-phosphate; not inhibited by D-glucose 6-phosphate; not inhibited by monovalent cations, glucose, ADPglucose, UDPglucose, NAD(H), NADP(H), dihydroxyacetone phosphate, glyceraldehyde 3-phosphate, 3-phosphoglycerate, CoA, acetyl-CoA, acetoacetyl-CoA; not inhibited by phosphate, AMP Clostridium pasteurianum
2.7.1.56 Ni2+ 3 mM, 50% inhibition in the presence of 3 mM Mg2+ Clostridium pasteurianum
2.7.1.56 SO42-
-
Clostridium pasteurianum
2.7.1.56 UTP if concentration exceeds Mg2+ concentration Clostridium pasteurianum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.56 0.14
-
ATP pH 8.0, 25°C Clostridium pasteurianum
2.7.1.56 0.62
-
D-Fructose 1-phosphate pH 8.0, 25°C Clostridium pasteurianum
2.7.1.56 0.67
-
ITP pH 8.0, 25°C Clostridium pasteurianum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.56 Co2+ 3 mM, 80% of activity with Mn2+ Clostridium pasteurianum
2.7.1.56 Fe2+ 3 mM, 50% of activity with Mn2+ Clostridium pasteurianum
2.7.1.56 Mg2+ required for activity Clostridium pasteurianum
2.7.1.56 Mg2+ 3 mM, 84% of activity with Mn2+ Clostridium pasteurianum
2.7.1.56 Mn2+ can replace Mg2+ Clostridium pasteurianum
2.7.1.56 Mn2+ required for activity Clostridium pasteurianum
2.7.1.56 Zn2+ 3 mM, 34% of activity with Mn2+ Clostridium pasteurianum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.56 21400
-
1 * 21400 + 1 * 35500, SDS-PAGE, after treatment with 8 M urea and 2-mercaptoethanol Clostridium pasteurianum
2.7.1.56 28500
-
1 * 28500 + 1 * 40000, SDS-PAGE, after treatment with 2-mercaptoethanol Clostridium pasteurianum
2.7.1.56 35500
-
1 * 21400 + 1 * 35500, SDS-PAGE, after treatment with 8 M urea and 2-mercaptoethanol Clostridium pasteurianum
2.7.1.56 40000
-
1 * 28500 + 1 * 40000, SDS-PAGE, after treatment with 2-mercaptoethanol Clostridium pasteurianum
2.7.1.56 62500
-
low-speed sedimentation equilibrium Clostridium pasteurianum
2.7.1.56 63100
-
gel filtration Clostridium pasteurianum
2.7.1.56 63200
-
sucrose density gradient centrifugation Clostridium pasteurianum

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.56 Clostridium pasteurianum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.56 CM-Sephadex, calcium phosphate gel, Sephadex G-100, DEAE-Sephadex, hydroxyapatite Clostridium pasteurianum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.56 additional information
-
-
Clostridium pasteurianum

Storage Stability

EC Number Storage Stability Organism
2.7.1.56 -20°C, 50 mM phosphate buffer, pH 7.5, 1 year, no loss of activity Clostridium pasteurianum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.56 ATP + D-fructose 1-phosphate
-
Clostridium pasteurianum ADP + D-fructose 1,6-bisphosphate
-
?
2.7.1.56 ITP + D-fructose 1-phosphate
-
Clostridium pasteurianum IDP + D-fructose 1,6-bisphosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.56 dimer 1 * 21400 + 1 * 35500, SDS-PAGE, after treatment with 8 M urea and 2-mercaptoethanol Clostridium pasteurianum
2.7.1.56 dimer 1 * 28500 + 1 * 40000, SDS-PAGE, after treatment with 2-mercaptoethanol Clostridium pasteurianum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.56 7.8 9.2
-
Clostridium pasteurianum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.56 7 9.8 approx. half-maximal activity at pH 7 and 9.8 Clostridium pasteurianum