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Literature summary extracted from

  • Richards, W.L.; Serif, G.S.
    Canine thyroid fucokinase (1977), Biochim. Biophys. Acta, 484, 353-367.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.52 EDTA stabilizes during purification Canis lupus familiaris
2.7.1.52 glycerol, sucrose, DTT or 2-mercaptoethanol stabilizes Canis lupus familiaris
2.7.1.52 ovalbumin does not stabilize purified enzyme Canis lupus familiaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.52 L-fucose substrate inhibition at high concentrations Canis lupus familiaris
2.7.1.52 L-galactose weak Canis lupus familiaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.52 0.017
-
L-fucose pH 6.5, 37°C Canis lupus familiaris
2.7.1.52 0.026
-
L-fucose pH 8.3, 37°C Canis lupus familiaris
2.7.1.52 0.29
-
ATP pH 6.5, pH 37°C Canis lupus familiaris
2.7.1.52 0.63
-
ATP pH 8.3, pH 37°C Canis lupus familiaris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.52 Co2+ divalent cation required, CoSO4 exhibits 12% of the activity with MgSO4 Canis lupus familiaris
2.7.1.52 Mg2+ divalent cation required Mg2+ is slightly more effective than Mn2+ Canis lupus familiaris
2.7.1.52 Mn2+ divalent cation required, MnSO4 exhibits 90% of the activity with MgSO4 Canis lupus familiaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.52 494000
-
gel filtration Canis lupus familiaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.52 ATP + 6-deoxy-L-galactose Canis lupus familiaris i.e. L-fucose, serves to utilize free L-fucose for glycoprotein synthesis ADP + 6-deoxy-L-galactose 1-phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.52 Canis lupus familiaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.52
-
Canis lupus familiaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.52 thyroid gland
-
Canis lupus familiaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.52 0.0091
-
radiometric assay Canis lupus familiaris

Storage Stability

EC Number Storage Stability Organism
2.7.1.52 2-4°C, most stable in the presence of glycerol and a sulfhydryl reagent Canis lupus familiaris
2.7.1.52 frozen, in the presence of glycerol or sucrose and a sulfhydryl reagent, up to 1 week Canis lupus familiaris
2.7.1.52 frozen, rapid loss of activity, glycerol or sucrose and DTT or 2-mercaptoethanol stabilize, no stabilization by Mg2+, EDTA, KCl or ovalbumin Canis lupus familiaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.52 ATP + 6-deoxy-L-galactose highly specific for ATP Canis lupus familiaris ADP + 6-deoxy-L-galactose 1-phosphate beta-L-fucose 1-phosphate ?
2.7.1.52 ATP + 6-deoxy-L-galactose highly specific for L-fucose Canis lupus familiaris ADP + 6-deoxy-L-galactose 1-phosphate beta-L-fucose 1-phosphate ?
2.7.1.52 ATP + 6-deoxy-L-galactose i.e. L-fucose, serves to utilize free L-fucose for glycoprotein synthesis Canis lupus familiaris ADP + 6-deoxy-L-galactose 1-phosphate
-
?
2.7.1.52 CTP + 6-deoxy-L-galactose no activity Canis lupus familiaris CDP + 6-deoxy-L-galactose 1-phosphate
-
?
2.7.1.52 GTP + 6-deoxy-L-galactose no activity Canis lupus familiaris GDP + 6-deoxy-L-galactose 1-phosphate
-
?
2.7.1.52 UTP + 6-deoxy-L-galactose no activity Canis lupus familiaris UDP + 6-deoxy-L-galactose 1-phosphate
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.52 6.5
-
two optima: pH 6.5 and pH 8.3 Canis lupus familiaris
2.7.1.52 8.3
-
two optima: pH 6.5 and pH 8.3 Canis lupus familiaris