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Literature summary extracted from

  • Cihak, A.
    Stability of the insoluble form of uridine kinase coupled to Zn2+ or Pb2+ ions (1976), Hoppe-Seyler's Z. Physiol. Chem., 357, 345-350.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.48 2-mercaptoethanol 1-2.5 mM, 40-50% activation of Pb2+-precipitated uridine kinase Bos taurus
2.7.1.48 dithiothreitol 1-2.5 mM, 40-50% activation of Pb2+-precipitated uridine kinase Bos taurus

General Stability

EC Number General Stability Organism
2.7.1.48 stability of insoluble metal ion precipitated form of the enzyme Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.48 Ag+
-
 Bos taurus
2.7.1.48 CTP 0.1 mM, complete inhibition Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.48 additional information freeze dried preparations of uridine kinase precipitated by Pb2+ or Zn2+ are highly active Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.48 Bos taurus
-
 calf
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.48 brain
-
 Bos taurus
-

Storage Stability

EC Number Storage Stability Organism
2.7.1.48 5°C, Pb2+ or Zn2+-precipitated uridine kinase, 6 months, 10-15% loss of activity Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.48 ATP + uridine
-
 Bos taurus ADP + UMP
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.48 50
-
 3% loss of activity after 10 min Bos taurus
2.7.1.48 100
-
 dry uridine kinase precipitated with Pb2+, 30% loss of activity after 1 h, dry uridine kinase precipitated with Zn2+, 25% loss of activity after 1 h Bos taurus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.48 4 7.5 complete loss of activity below pH 4.0 Bos taurus