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Literature summary extracted from

  • Fulchignoni-Lataud, M.C.; Tuilie, M.; Roux, J.M.
    Uridine--cytidine kinase from foetal and adult rat liver. Purification and study of some properties (1976), Eur. J. Biochem., 69, 217-222.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.48 ATP protects the enzyme completely for 30 min at 37°C in crude extract Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.48 0.052
-
 uridine 37°C, adult liver uridine kinase Rattus norvegicus
2.7.1.48 0.37
-
 ATP 37°C, foetal liver uridine kinase Rattus norvegicus
2.7.1.48 0.37
-
 ATP ATP in form of MgATP2- Rattus norvegicus
2.7.1.48 0.4
-
 uridine 37°C, foetal liver uridine kinase Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.48 Mg2+ required for full activity Rattus norvegicus
2.7.1.48 Mg2+ optimal Mg2+ to ATP ratio at 12 mM ATP: 1.25 Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.48 Rattus norvegicus
-
 2 forms of liver uridine kinase in foetus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.48 streptomycin, ammonium sulfate, DEAE-cellulose Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.48 liver foetal and adult Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.48 0.06
-
 uridine kinase from adult animal Rattus norvegicus
2.7.1.48 57.49
-
 uridine kinase from foetal animal Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.7.1.48 -20°C, several weeks, no loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.48 ATP + uridine
-
 Rattus norvegicus ADP + UMP
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.48 additional information
-
 ATP protects foetal liver uridine kinase completely for 30 min at 37°C in crude extract Rattus norvegicus
2.7.1.48 45
-
 foetal liver uridine kinase, 50% loss of activity after 10 min, adult liver uridine kinase, no loss of activity after 10 min Rattus norvegicus