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Literature summary extracted from

  • Payne, R.C.; Cheng, N.; Traut, T.W.
    Uridine kinase from Ehrlich ascites carcinoma. Purification and properties of homogeneous enzyme (1985), J. Biol. Chem., 260, 10242-10247.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.48 pure enzyme is very sensitive to freezing Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.48 CTP
-
 Mus musculus
2.7.1.48 UTP
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.48 0.04
-
 uridine pH 8.0, 22°C Mus musculus
2.7.1.48 0.057
-
 cytidine pH 8.0, 22°C Mus musculus
2.7.1.48 0.069
-
 5-fluorouridine pH 8.0, 22°C Mus musculus
2.7.1.48 0.2
-
 3-deazauridine pH 8.0, 22°C Mus musculus
2.7.1.48 0.34
-
 6-azauridine pH 8.0, 22°C Mus musculus
2.7.1.48 0.45
-
 ATP pH 8.0, 22°C Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.48 31000
-
 x * 31000, enzyme exists in multiple interconvertible oligomeric forms, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.48 ATP + uridine Mus musculus rate limiting enzyme of pyrimidine salvage pathway ADP + UMP
-
 ?
2.7.1.48 ATP + uridine Mus musculus rate-limiting enzyme in anabolism of uridine and cytidine ADP + UMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.48 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.48 DE-52, ammonium sulfate, ATP-agarose column Mus musculus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.48 ATP + uridine = ADP + UMP sequential mechanism Mus musculus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.48 Ehrlich ascites carcinoma cell
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.48 283
-
-
 Mus musculus

Storage Stability

EC Number Storage Stability Organism
2.7.1.48 -20°C, 50% glycerol, several months, no loss of activity Mus musculus
2.7.1.48 0°C, 8 weeks, 20% loss of activity Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.48 ATP + 3-deazauridine
-
 Mus musculus ADP + 3-deazauridine 5'-monophosphate
-
 ?
2.7.1.48 ATP + 5-fluorouridine
-
 Mus musculus ADP + 5-fluorouridine 5'-monophosphate
-
 ?
2.7.1.48 ATP + 6-azauridine
-
 Mus musculus ADP + 6-azauridine 5'-monophosphate
-
 ?
2.7.1.48 ATP + cytidine
-
 Mus musculus ADP + CMP
-
 ?
2.7.1.48 ATP + uridine
-
 Mus musculus ADP + UMP
-
 ?
2.7.1.48 ATP + uridine rate limiting enzyme of pyrimidine salvage pathway Mus musculus ADP + UMP
-
 ?
2.7.1.48 ATP + uridine rate-limiting enzyme in anabolism of uridine and cytidine Mus musculus ADP + UMP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.48 ? x * 31000, enzyme exists in multiple interconvertible oligomeric forms, SDS-PAGE Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.48 22
-
 assay at Mus musculus
2.7.1.48 37
-
 assay at Mus musculus