EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.48 | CTP | enzyme shows a temperature dependence of CTP inhibition | Geobacillus stearothermophilus | |
2.7.1.48 | CTP | 0.56 mM, 50% inhibition | Rattus norvegicus | |
2.7.1.48 | UTP | - |
Geobacillus stearothermophilus | |
2.7.1.48 | UTP | - |
Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.48 | 0.21 | - |
uridine | pH 7.5, 10°C | Geobacillus stearothermophilus | |
2.7.1.48 | 0.64 | - |
uridine | pH 7.5, 37°C | Geobacillus stearothermophilus | |
2.7.1.48 | 1.6 | - |
uridine | pH 7.5, 60°C | Geobacillus stearothermophilus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.48 | Mg2+ | required for full activity | Rattus norvegicus | |
2.7.1.48 | Mg2+ | required for full activity | Geobacillus stearothermophilus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.48 | ATP + uridine | Rattus norvegicus | first enzyme of pyrimidine salvage pathway | ADP + UMP | - |
? | |
2.7.1.48 | ATP + uridine | Geobacillus stearothermophilus | first enzyme of pyrimidine salvage pathway | ADP + UMP | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.48 | Geobacillus stearothermophilus | - |
- |
- |
2.7.1.48 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.48 | - |
Geobacillus stearothermophilus |
2.7.1.48 | streptomycin, ammonium sulfate, calcium phosphate, Sephadex G-200 | Rattus norvegicus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.7.1.48 | ATP + uridine = ADP + UMP | ping-pong mechanism | Rattus norvegicus | |
2.7.1.48 | ATP + uridine = ADP + UMP | ping-pong mechanism | Geobacillus stearothermophilus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.1.48 | Novikoff ascites tumor cell | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.48 | 0.88 | - |
- |
Rattus norvegicus |
2.7.1.48 | 4.33 | - |
- |
Geobacillus stearothermophilus |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.1.48 | -20°C, 50% glycerol, at least 6 months, no loss of activity | Rattus norvegicus |
2.7.1.48 | 4°C, at least 1 month, no loss of activity | Rattus norvegicus |
2.7.1.48 | 4°C, sterile conditions, at least 2 years, no loss of activity enzyme | Geobacillus stearothermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.48 | ATP + uridine | very low activity with CTP and UTP | Rattus norvegicus | ADP + UMP | - |
? | |
2.7.1.48 | ATP + uridine | very low activity with CTP and UTP | Geobacillus stearothermophilus | ADP + UMP | - |
? | |
2.7.1.48 | ATP + uridine | first enzyme of pyrimidine salvage pathway | Rattus norvegicus | ADP + UMP | - |
? | |
2.7.1.48 | ATP + uridine | first enzyme of pyrimidine salvage pathway | Geobacillus stearothermophilus | ADP + UMP | - |
? | |
2.7.1.48 | dATP + uridine | - |
Rattus norvegicus | dADP + UMP | - |
? | |
2.7.1.48 | dATP + uridine | - |
Geobacillus stearothermophilus | dADP + UMP | - |
? | |
2.7.1.48 | dCTP + uridine | weak activity | Rattus norvegicus | dCDP + UMP | - |
? | |
2.7.1.48 | dCTP + uridine | weak activity | Geobacillus stearothermophilus | dCDP + UMP | - |
? | |
2.7.1.48 | dGTP + uridine | - |
Rattus norvegicus | dGDP + UMP | - |
? | |
2.7.1.48 | dGTP + uridine | - |
Geobacillus stearothermophilus | dGDP + UMP | - |
? | |
2.7.1.48 | dTTP + uridine | weak activity | Rattus norvegicus | dTDP + UMP | - |
? | |
2.7.1.48 | dTTP + uridine | weak activity | Geobacillus stearothermophilus | dTDP + UMP | - |
? | |
2.7.1.48 | dUTP + uridine | - |
Rattus norvegicus | dUDP + UMP | - |
? | |
2.7.1.48 | dUTP + uridine | - |
Geobacillus stearothermophilus | dUDP + UMP | - |
? | |
2.7.1.48 | GTP + uridine | weak activity | Rattus norvegicus | UMP + GDP | - |
? | |
2.7.1.48 | GTP + uridine | weak activity | Geobacillus stearothermophilus | UMP + GDP | - |
? | |
2.7.1.48 | ITP + uridine | weak activity | Rattus norvegicus | IDP + UMP | - |
? | |
2.7.1.48 | ITP + uridine | weak activity | Geobacillus stearothermophilus | IDP + UMP | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.48 | 37 | - |
assay at | Rattus norvegicus |
2.7.1.48 | 37 | - |
assay at | Geobacillus stearothermophilus |
2.7.1.48 | 60 | - |
higher activity than at 10°C and 37°C | Geobacillus stearothermophilus |