Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Orengo, A.; Saunders, G.F.
    Regulation of a thermostable pyrimidine ribonucleoside kinase by cytidine triphosphate (1972), Biochemistry, 11, 1761-1767.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.48 CTP 80-85% inhibition at approx. 2 mM Geobacillus stearothermophilus
2.7.1.48 CTP complete inhibition at approx. 2 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.48 0.21
-
 uridine
-
 Geobacillus stearothermophilus
2.7.1.48 0.27
-
 uridine 37°C Rattus norvegicus
2.7.1.48 0.64
-
 uridine
-
 Geobacillus stearothermophilus
2.7.1.48 1.6
-
 uridine
-
 Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.48 109000
-
 sucrose density gradient centrifugation Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.48 Geobacillus stearothermophilus
-
-
-
2.7.1.48 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.48 streptomycin, ammonium sulfate, calcium phosphate, DEAE-cellulose Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.48 ATP + uridine
-
 Rattus norvegicus ADP + UMP
-
 ?
2.7.1.48 ATP + uridine
-
 Geobacillus stearothermophilus ADP + UMP
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.48 60
-
 higher activity than at 10°C and 37°C Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.48 59.5
-
 no loss of activity after 30 min Geobacillus stearothermophilus
2.7.1.48 59.5
-
 79% loss of activity after 4 min Rattus norvegicus