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Literature summary extracted from

  • Sköld, O.
    Uridine kinase from ehrlich ascites tumor: purification and properties (1960), J. Biol. Chem., 235, 3273-3279.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.48 additional information no substrate inhibition of cytidine and 5-fluorouridine at substrate concentrations higher than 0.5 mM Mus musculus
2.7.1.48 p-chloromercuribenzoate 0.17 mM, 67% inhibition, 0.69 mM, 82% inhibition, glutathione protects Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.48 0.023
-
 cytidine pH 7.4, 37°C Mus musculus
2.7.1.48 0.03
-
 5-fluorouridine pH 7.4, 37°C Mus musculus
2.7.1.48 0.048
-
 uridine pH 7.4, 37°C Mus musculus
2.7.1.48 0.5
-
 ATP pH 7.4, 37°C Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.48 Fe2+ can partially replace Mg2+ Mus musculus
2.7.1.48 Fe2+ 59% of activity with Mg2+ Mus musculus
2.7.1.48 Mg2+ required for full activity Mus musculus
2.7.1.48 Mg2+ between 0.5/1 and 1/1 Mus musculus
2.7.1.48 Mn2+ can partially replace Mg2+ Mus musculus
2.7.1.48 Mn2+ 44 mM, 68% of activity with Mg2+ Mus musculus
2.7.1.48 additional information not activated by Ca2+ and Cu2+ Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.48 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.48 protamine sulfate, CM-cellulose, ammonium sulfate, DEAE-cellulose, zone electrophoresis Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.48 Ehrlich ascites carcinoma cell
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.48 80.5
-
-
 Mus musculus

Storage Stability

EC Number Storage Stability Organism
2.7.1.48 -15°C, 2 to 3 months, occasional thawing, no loss of activity Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.48 ATP + 5-fluorocytidine
-
 Mus musculus ADP + 5-fluorocytidine 5'-monophosphate
-
 ?
2.7.1.48 ATP + 5-fluorouridine
-
 Mus musculus ADP + 5-fluorouridine 5'-monophosphate
-
 ?
2.7.1.48 ATP + 6-azauridine
-
 Mus musculus ADP + 6-azauridine 5'-monophosphate
-
 ?
2.7.1.48 ATP + cytidine
-
 Mus musculus ADP + CMP
-
 ?
2.7.1.48 ATP + uridine no activity with CTP and UTP Mus musculus ADP + UMP
-
 ?
2.7.1.48 GTP + uridine
-
 Mus musculus UMP + GDP
-
 ?
2.7.1.48 ITP + uridine
-
 Mus musculus IDP + UMP
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.48 37
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.48 5.5 8
-
 Mus musculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.48 5 9 approx. 50% of maximal activity at pH 5.0, approx. 70% of maximal activity at pH 9.0 Mus musculus