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Literature summary extracted from

  • Stayton, M.M.; Fromm, H.J.
    Purification, properties, and kinetics of D-ribulokinase from Aerobacter aerogenes (1979), J. Biol. Chem., 254, 3765-3771.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.47 2-mercaptoethanol activation Klebsiella aerogenes

General Stability

EC Number General Stability Organism
2.7.1.47 D-ribulose stabilizes Klebsiella aerogenes
2.7.1.47 EDTA or 2-mercaptoethanol stabilizes during purification Klebsiella aerogenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.47 ADP product inhibition Klebsiella aerogenes
2.7.1.47 additional information no inhibition by D-ribulose 5-phosphate Klebsiella aerogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.47 additional information
-
 additional information kinetic study at 28°C Klebsiella aerogenes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.47 Mg2+ requirement Klebsiella aerogenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.47 59000
-
 2 * 59000, SDS-PAGE Klebsiella aerogenes
2.7.1.47 116000
-
 gel filtration Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.47 ATP + D-ribulose Klebsiella aerogenes inducible enzyme of ribitol catabolism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.47 Klebsiella aerogenes
-
 mutant, constitutive for D-ribulokinase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.47
-
 Klebsiella aerogenes

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.47 ATP + D-ribulose = ADP + D-ribulose 5-phosphate random bi bi reaction mechanism Klebsiella aerogenes
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.47 152
-
-
 Klebsiella aerogenes

Storage Stability

EC Number Storage Stability Organism
2.7.1.47 4°C, about 10% loss of activity within 6 months Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.47 ATP + D-ribulose
-
 Klebsiella aerogenes ADP + D-ribulose 5-phosphate
-
 ?
2.7.1.47 ATP + D-ribulose inducible enzyme of ribitol catabolism Klebsiella aerogenes ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.47 dimer 2 * 59000, SDS-PAGE Klebsiella aerogenes