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Literature summary extracted from

  • Mesak, L.R.; Dahl, M.K.
    Purification and enzymatic characterization of PgcM: A beta-phosphoglucomutase and glucose-1-phosphate phosphodismutase of Bacillus subtilis (2000), Arch. Microbiol., 174, 256-264.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.4.2.6 beta-D-glucose 1,6-bisphosphate
-
 Bacillus subtilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.41 pgcM gene, encoding PgcM, a 226 amino acids protein with beta-phosphoglucomutase and glucose 1-phosphate phosphodismutase activities, is cloned, sequenced and used to construct a plasmid-based overexpression system for PcgM in Bacillus megaterium DSM 319 Bacillus subtilis
5.4.2.6 overexpression in Bacillus mergaterium Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.41 0.006
-
 beta-D-glucose 1-phosphate
-
 Bacillus subtilis
2.7.1.41 0.021
-
 alpha-D-glucose 1-phosphate
-
 Bacillus subtilis
5.4.2.6 0.004
-
 beta-D-glucose 1-phosphate phosphoglucomutase activity Bacillus subtilis
5.4.2.6 0.006
-
 beta-D-glucose 1-phosphate 1-phosphate phosphodismutase activity Bacillus subtilis
5.4.2.6 0.021
-
 alpha-D-glucose 1-phosphate 1-phosphate phosphodismutase activity Bacillus subtilis
5.4.2.6 0.027
-
 alpha-D-glucose 1-phosphate phosphoglucomutase activity Bacillus subtilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.41 soluble recombinant PgcM with beta-phosphoglucomutase and glucose 1-phosphate phosphodismutase activities Bacillus subtilis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.41 Mg2+ MgCl2 as cofactor, optimal concentration: 10-15 mM Bacillus subtilis
5.4.2.6 Mg2+ required for activity, maximal activity at 10 mM Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.41 26500
-
 3 * 26500, PgcM with beta-phosphoglucomutase and glucose 1-phosphate phosphodismutase activities, SDS-PAGE Bacillus subtilis
2.7.1.41 28000
-
 3 * 28000, PgcM with beta-phosphoglucomutase and glucose 1-phosphate phosphodismutase activities, estimated from the amino acid sequence data Bacillus subtilis
5.4.2.6 26500
-
 3 * 26500, SDS-PAGE Bacillus subtilis
5.4.2.6 80000
-
 nonreducing SDS-PAGE Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.41 additional information Bacillus subtilis carbohydrate metabolism ?
-
 ?
5.4.2.6 beta-D-Glucose 1-phosphate Bacillus subtilis
-
 beta-D-Glucose 6-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.41 Bacillus subtilis
-
 wild-type strain 168
-
5.4.2.6 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.41 recombinant PgcM expressed in Bacillus megaterium DSM 319 Bacillus subtilis
5.4.2.6 recombinant PgcM Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.41 alpha-D-glucose 1-phosphate + alpha-D-glucose 1-phosphate
-
 Bacillus subtilis alpha-D-glucose + alpha-D-glucose 1,6-bisphosphate
-
 ?
2.7.1.41 beta-D-glucose 1-phosphate + beta-D-glucose 1-phosphate
-
 Bacillus subtilis beta-D-glucose + beta-D-glucose 1,6-bisphosphate
-
 ?
2.7.1.41 D-glucose 1-phosphate + D-glucose 1-phosphate PgcM uses the alpha- and beta-forms of glucose 1-phosphate as substrates, higher affinity for the beta-form, binding of substrates and cofactors trigger a conformational change of PgcM Bacillus subtilis D-glucose + D-glucose 1,6-bisphosphate
-
 ?
2.7.1.41 additional information bifunctional PgcM with beta-phosphoglucomutase, EC 5.4.2.1, and glucose 1-phosphate phosphodismutase activities Bacillus subtilis ?
-
 ?
2.7.1.41 additional information carbohydrate metabolism Bacillus subtilis ?
-
 ?
5.4.2.6 alpha-D-glucose 1-phosphate 7fold lower affinity than with beta-D-glucose Bacillus subtilis alpha-D-glucose 6-phosphate
-
 r
5.4.2.6 beta-D-Glucose 1-phosphate
-
 Bacillus subtilis beta-D-Glucose 6-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.1.41 trimer 3 * 28000, PgcM with beta-phosphoglucomutase and glucose 1-phosphate phosphodismutase activities, estimated from the amino acid sequence data Bacillus subtilis
2.7.1.41 trimer 3 * 26500, PgcM with beta-phosphoglucomutase and glucose 1-phosphate phosphodismutase activities, SDS-PAGE Bacillus subtilis
5.4.2.6 trimer 3 * 26500, SDS-PAGE Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
2.7.1.41 PgcM bifunctional PgcM with beta-phosphoglucomutase, EC 5.4.2.1, and glucose 1-phosphate phosphodismutase activities Bacillus subtilis
5.4.2.6 PgcM
-
 Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.41 95
-
 30 min, stable, PgcM with beta-phosphoglucomutase and glucose 1-phosphate phosphodismutase activities Bacillus subtilis
5.4.2.6 95
-
 no loss of activity after 30 min Bacillus subtilis