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Literature summary extracted from

  • Maeda, T.; Saito, T.; Oguchi, Y.; Nakazawa, M.; Takeuchi, T.; Asai, T.
    Expression and characterization of recombinant pyruvate kinase from Toxoplasma gondii tachyzoites (2003), Parasitol. Res., 89, 259-265.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.40 D-fructose 1,6-diphosphate not Toxoplasma gondii
2.7.1.40 D-glucose 6-phosphate radically activates Toxoplasma gondii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.40
-
 Toxoplasma gondii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.40 AMP not Toxoplasma gondii
2.7.1.40 MgATP2-
-
 Toxoplasma gondii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.40 57000
-
 4 * 57000, SDS-PAGE Toxoplasma gondii
2.7.1.40 220000 240000 gel filtration Toxoplasma gondii

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Toxoplasma gondii Q969A2
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Storage Stability

EC Number Storage Stability Organism
2.7.1.40 4°C, in 20 mM Tris-HCl, pH 7.0, 30% glycerol, 200 mM KCl, stable for 1 week Toxoplasma gondii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate
-
 Toxoplasma gondii ATP + pyruvate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.40 tetramer 4 * 57000, SDS-PAGE Toxoplasma gondii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.40 additional information
-
 no loss with one cycle of freezing and thawing Toxoplasma gondii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.40 7
-
-
 Toxoplasma gondii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.40 1.77
-
 MgATP2- 37°C, pH 7.0 Toxoplasma gondii