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Literature summary extracted from

  • Turner, W.L.; Plaxton, W.C.
    Purification and characterization of cytosolic pyruvate kinase from banana fruit (2000), Biochem. J., 352, 875-882.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.40 L-glutamate
-
 Musa cavendishii
2.7.1.40 MgATP2-
-
 Musa cavendishii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.40 0.098
-
 phosphoenolpyruvate hyperbolic saturation kinetics, pH 6.9, 25°C Musa cavendishii
2.7.1.40 0.12
-
 ADP hyperbolic saturation kinetics, pH 6.9, 25°C Musa cavendishii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.40 K+ requirement Musa cavendishii
2.7.1.40 K+ Km-value 0.91 mM, hyperbolic saturation kinetics Musa cavendishii
2.7.1.40 Mg2+ Km-value 0.27 mM, hyperbolic saturation kinetics Musa cavendishii
2.7.1.40 Mg2+ fulfills absolute requirement for divalent cation Musa cavendishii
2.7.1.40 additional information absolute requirement for a bivalent and a monovalent cation with Mg2+ and K+ fulfilling this Musa cavendishii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.40 57000
-
 4 * 57000, SDS-PAGE Musa cavendishii
2.7.1.40 240000
-
 gel filtration Musa cavendishii

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Musa cavendishii
-
 banana
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.40
-
 Musa cavendishii

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.40 ATP + pyruvate = ADP + phosphoenolpyruvate model for allosteric regulation Musa cavendishii
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.40 fruit
-
 Musa cavendishii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.40 59
-
 pH 6.9, 25°C Musa cavendishii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate ADP preferred substrate, UDP, IDP, GDP and CDP may also be used with lower effectivity Musa cavendishii ATP + pyruvate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.40 tetramer 4 * 57000, SDS-PAGE Musa cavendishii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.40 6.9
-
-
 Musa cavendishii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.40 3.7
-
 L-glutamate cosubstrate MgADP-, pH 7.5, 25°C Musa cavendishii
2.7.1.40 3.9
-
 MgATP2- cosubstrate MgADP-, pH 6.9, 25°C Musa cavendishii
2.7.1.40 4
-
 MgATP2- cosubstrate MgADP-, pH 7.5, 25°C Musa cavendishii
2.7.1.40 4.4
-
 L-glutamate cosubstrate phosphoenolpyruvate, pH 7.5, 25°C Musa cavendishii
2.7.1.40 9.6
-
 MgATP2- cosubstrate phosphoenolpyruvate, pH 6.9, 25°C Musa cavendishii
2.7.1.40 10
-
 MgATP2- cosubstrate phosphoenolpyruvate, pH 7.5, 25°C Musa cavendishii
2.7.1.40 13.6
-
 L-glutamate cosubstrate phosphoenolpyruvate, pH 6.9, 25°C Musa cavendishii
2.7.1.40 19.2
-
 L-glutamate cosubstrate MgADP-, pH 6.9, 25°C Musa cavendishii