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Literature summary extracted from

  • Kapoor, R.; Venkitasubramanian, T.A.
    Purification and properties of pyruvate kinase from Mycobacterium smegmatis (1983), Arch. Biochem. Biophys., 225, 320-330.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.40 D-glucose 6-phosphate activation Mycolicibacterium smegmatis

General Stability

EC Number General Stability Organism
2.7.1.40 0.1 M KCl stabilizes enzyme in dilute solutions Mycolicibacterium smegmatis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.40 ATP glucose 6-phosphate reverses Mycolicibacterium smegmatis
2.7.1.40 Ba2+ in decreasing order of inhibitory efficiency: Ni2+, Zn2+, Cu2+, Ca2+, Ba2+ Mycolicibacterium smegmatis
2.7.1.40 Ca2+ in decreasing order of inhibitory efficiency: Ni2+, Zn2+, Cu2+, Ca2+, Ba2+ Mycolicibacterium smegmatis
2.7.1.40 Cu2+ in decreasing order of inhibitory efficiency: Ni2+, Zn2+, Cu2+, Ca2+, Ba2+ Mycolicibacterium smegmatis
2.7.1.40 Ni2+ in decreasing order of inhibitory efficiency: Ni2+, Zn2+, Cu2+, Ca2+, Ba2+ Mycolicibacterium smegmatis
2.7.1.40 phosphate
-
 Mycolicibacterium smegmatis
2.7.1.40 sulfate
-
 Mycolicibacterium smegmatis
2.7.1.40 Zn2+ in decreasing order of inhibitory efficiency: Ni2+, Zn2+, Cu2+, Ca2+, Ba2+ Mycolicibacterium smegmatis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.40 additional information
-
 additional information kinetic parameters Mycolicibacterium smegmatis
2.7.1.40 0.04 0.063 phosphoenolpyruvate
-
 Mycolicibacterium smegmatis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.40 Ca2+ not Mycolicibacterium smegmatis
2.7.1.40 Mg2+ requirement Mycolicibacterium smegmatis
2.7.1.40 Mg2+ kinetics Mycolicibacterium smegmatis
2.7.1.40 Mn2+ requirement Mycolicibacterium smegmatis
2.7.1.40 Mn2+ can partially replace Mg2+ Mycolicibacterium smegmatis
2.7.1.40 Mn2+ kinetics Mycolicibacterium smegmatis
2.7.1.40 monovalent cation not Mycolicibacterium smegmatis
2.7.1.40 additional information no activation by Ba2+ Mycolicibacterium smegmatis
2.7.1.40 additional information no activation by Cu2+, Ni2+ Mycolicibacterium smegmatis
2.7.1.40 Zn2+ not Mycolicibacterium smegmatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.40 230700
-
 gel filtration Mycolicibacterium smegmatis

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Mycolicibacterium smegmatis
-
-
-
2.7.1.40 Mycolicibacterium smegmatis CDC 46
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.40
-
 Mycolicibacterium smegmatis

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.40 ATP + pyruvate = ADP + phosphoenolpyruvate allosteric enzyme: homotropic Mycolicibacterium smegmatis
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.40 cell culture
-
 Mycolicibacterium smegmatis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.40 220
-
-
 Mycolicibacterium smegmatis

Storage Stability

EC Number Storage Stability Organism
2.7.1.40 -20°C, in 50 mM phosphate buffer, pH 7, 100 mM KCl, 2 mM phosphoenolpyruvate, 0.1% 2-mercaptoethanol, 50% v/v glycerol, at least 6 months Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate best nucleoside diphosphate substrate Mycolicibacterium smegmatis ATP + pyruvate
-
 ?
2.7.1.40 ADP + phosphoenolpyruvate positive cooperativity for phosphoenolpyruvate Mycolicibacterium smegmatis ATP + pyruvate
-
 ?
2.7.1.40 ADP + phosphoenolpyruvate best nucleoside diphosphate substrate Mycolicibacterium smegmatis CDC 46 ATP + pyruvate
-
 ?
2.7.1.40 ADP + phosphoenolpyruvate positive cooperativity for phosphoenolpyruvate Mycolicibacterium smegmatis CDC 46 ATP + pyruvate
-
 ?
2.7.1.40 CDP + phosphoenolpyruvate less effective than ADP Mycolicibacterium smegmatis CTP + pyruvate
-
 ?
2.7.1.40 CDP + phosphoenolpyruvate less effective than ADP Mycolicibacterium smegmatis CDC 46 CTP + pyruvate
-
 ?
2.7.1.40 additional information allosteric enzyme: homotropic Mycolicibacterium smegmatis ?
-
 ?
2.7.1.40 additional information allosteric enzyme: homotropic Mycolicibacterium smegmatis CDC 46 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.40 tetramer 4* 57540, SDS-PAGE Mycolicibacterium smegmatis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.40 28
-
 assay at Mycolicibacterium smegmatis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.40 30
-
 up to, in 50 mM HEPES-potassium hydroxide buffer, pH 7.5, 0.1 M mercaptoethanol, 20% v/v glycerol, 30 min stable Mycolicibacterium smegmatis
2.7.1.40 70
-
 30 min, in 50 mM HEPES-potassium hydroxide buffer, pH 7.5, 0.1 M 2-mercaptoethanol, 20% v/v glycerol, 85% loss of activity Mycolicibacterium smegmatis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.40 7
-
-
 Mycolicibacterium smegmatis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.40 6.8 7.1
-
 Mycolicibacterium smegmatis