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Literature summary extracted from

  • De Médicis, E.; Laliberté, J.F.; Vass-Marengo, J.
    Purification and properties of pyruvate kinase from Halobacterium cutirubrum (1982), Biochim. Biophys. Acta, 708, 57-67.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.40 alanine not Halobacterium salinarum
2.7.1.40 D-ribose 5-phosphate activation Halobacterium salinarum
2.7.1.40 Monovalent anions activation, in decreasing order of efficiency: Cl-, Br-, NO3- Halobacterium salinarum

General Stability

EC Number General Stability Organism
2.7.1.40 highly unstable at less than 3 M sodium or potassium chloride, cannot be renatured Halobacterium salinarum
2.7.1.40 optimal stability at pH 6.5 and room temperature Halobacterium salinarum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.40 6-phosphogluconate
-
 Halobacterium salinarum
2.7.1.40 fructose 1,6-diphosphate
-
 Halobacterium salinarum
2.7.1.40 L-alanine not Halobacterium salinarum
2.7.1.40 phosphate
-
 Halobacterium salinarum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.40 K+ requirement Halobacterium salinarum
2.7.1.40 K+ best activator at optimal conditions, in decreasing order of efficiency: K+, Rb+, Cs+, Na+, NH4+, Li+ Halobacterium salinarum
2.7.1.40 monovalent cation in decreasing order of efficiency: K+, Rb+, Cs+, Na+, NH4+, Li+ Halobacterium salinarum
2.7.1.40 additional information no activation by SCN- Halobacterium salinarum
2.7.1.40 Na+ in decreasing order of efficiency: K+, Rb+, Cs+, Na+, NH4+, Li+ Halobacterium salinarum
2.7.1.40 NH4+ in decreasing order of efficiency: K+, Rb+, Cs+, Na+, NH4+, Li+ Halobacterium salinarum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.40 73000
-
 4 * 73000, SDS-PAGE Halobacterium salinarum
2.7.1.40 300000
-
 gel filtration Halobacterium salinarum

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Halobacterium salinarum
-
-
-
2.7.1.40 Halobacterium salinarum 9
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.40 at room temperature, partial Halobacterium salinarum

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.40 ATP + pyruvate = ADP + phosphoenolpyruvate allosteric enzyme: homotropic Halobacterium salinarum
a

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.7.1.40 the enzyme cannot be renatured Halobacterium salinarum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.40 cell culture
-
 Halobacterium salinarum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.40 additional information
-
-
 Halobacterium salinarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate positive cooperativity for phosphoenolpyruvate Halobacterium salinarum ATP + pyruvate
-
 ?
2.7.1.40 ADP + phosphoenolpyruvate positive cooperativity for phosphoenolpyruvate Halobacterium salinarum 9 ATP + pyruvate
-
 ?
2.7.1.40 additional information allosteric enzyme: homotropic Halobacterium salinarum ?
-
 ?
2.7.1.40 additional information allosteric enzyme: homotropic Halobacterium salinarum 9 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.40 tetramer 4 * 73000, SDS-PAGE Halobacterium salinarum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.40 additional information
-
 cold-labile enzyme Halobacterium salinarum
2.7.1.40 20
-
 most stable at Halobacterium salinarum
2.7.1.40 50
-
 45 min, 15% loss of activity Halobacterium salinarum
2.7.1.40 60
-
 t1/2: 10 min Halobacterium salinarum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.40 6.5
-
 stable at Halobacterium salinarum

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.40 AMP allosteric effector Halobacterium salinarum