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Literature summary extracted from

  • Sakai, H.; Suzuki, K.; Imahori, K.
    Purification and properties of pyruvate kinase from Bacillus stearothermophilus (1986), J. Biochem., 99, 1157-1167.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.40 3-phosphoglycerate slight activation Geobacillus stearothermophilus
2.7.1.40 D-fructose 1,6-diphosphate not Geobacillus stearothermophilus
2.7.1.40 D-glucose 1-phosphate not Geobacillus stearothermophilus
2.7.1.40 D-glucose 6-phosphate activation Geobacillus stearothermophilus
2.7.1.40 D-ribose 5-phosphate activation Geobacillus stearothermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.40 ADP at high concentrations; substrate inhibition Geobacillus stearothermophilus
2.7.1.40 Ca2+
-
 Geobacillus stearothermophilus
2.7.1.40 Cu2+
-
 Geobacillus stearothermophilus
2.7.1.40 Mg2+ free form Geobacillus stearothermophilus
2.7.1.40 Ni2+
-
 Geobacillus stearothermophilus
2.7.1.40 phosphate
-
 Geobacillus stearothermophilus
2.7.1.40 Sr2+
-
 Geobacillus stearothermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.40 Ca2+ not Geobacillus stearothermophilus
2.7.1.40 Co2+ can replace Mg2+ Geobacillus stearothermophilus
2.7.1.40 Co2+ requirement Geobacillus stearothermophilus
2.7.1.40 K+ requirement Geobacillus stearothermophilus
2.7.1.40 Mg2+ requirement Geobacillus stearothermophilus
2.7.1.40 Mn2+ requirement Geobacillus stearothermophilus
2.7.1.40 Mn2+ can partially replace Mg2+ Geobacillus stearothermophilus
2.7.1.40 additional information no activation by Sr2+ Geobacillus stearothermophilus
2.7.1.40 additional information no activation by Cu2+, Ni2+ Geobacillus stearothermophilus
2.7.1.40 Na+ can poorly replace K+ Geobacillus stearothermophilus
2.7.1.40 NH4+ requirement Geobacillus stearothermophilus
2.7.1.40 NH4+ can replace K+ Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.40 additional information
-
 amino acid composition Geobacillus stearothermophilus
2.7.1.40 242000
-
 sedimentation analysis Geobacillus stearothermophilus
2.7.1.40 250000
-
 gel filtration Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Geobacillus stearothermophilus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.40 additional information no glycoprotein Geobacillus stearothermophilus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.40 no isozymes Geobacillus stearothermophilus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.40 ATP + pyruvate = ADP + phosphoenolpyruvate allosteric enzyme: homotropic Geobacillus stearothermophilus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.40 cell culture
-
 Geobacillus stearothermophilus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.40 333
-
 pH 7.2, 30°C Geobacillus stearothermophilus

Storage Stability

EC Number Storage Stability Organism
2.7.1.40 -20°C, in 25 mM Tris-HCl buffer, pH 7.5, 50 mM KCl, 5 mM MgCl2, 50% ethylene glycol, at least 2 years Geobacillus stearothermophilus
2.7.1.40 25°C, in 25 mM Tris-HCl buffer, pH 7.5, 50 mM KCl, 5 mM MgCl2, 50% ethylene glycol, inactivation within a few days Geobacillus stearothermophilus
2.7.1.40 4°C, in 25 mM Tris-HCl buffer, pH 7.5, 50 mM KCl, 5 mM MgCl2, 50% ethylene glycol, at least 2 years, at pH-values below 5, inactivation within a few days Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate no positive cooperativity for ADP Geobacillus stearothermophilus ATP + pyruvate
-
 ?
2.7.1.40 ADP + phosphoenolpyruvate best nucleoside diphosphate substrate Geobacillus stearothermophilus ATP + pyruvate
-
 ?
2.7.1.40 ADP + phosphoenolpyruvate positive cooperativity for phosphoenolpyruvate Geobacillus stearothermophilus ATP + pyruvate
-
 ?
2.7.1.40 CDP + phosphoenolpyruvate poor substrate Geobacillus stearothermophilus CTP + pyruvate
-
 ?
2.7.1.40 CDP + phosphoenolpyruvate less effective than ADP Geobacillus stearothermophilus CTP + pyruvate
-
 ?
2.7.1.40 epsilon-ADP + phosphoenolpyruvate poor substrate Geobacillus stearothermophilus ?
-
 ?
2.7.1.40 GDP + phosphoenolpyruvate less effective than ADP Geobacillus stearothermophilus GTP + pyruvate
-
 ?
2.7.1.40 IDP + phosphoenolpyruvate less effective than ADP Geobacillus stearothermophilus ITP + pyruvate
-
 ?
2.7.1.40 additional information broad specificity for nucleoside diphosphates Geobacillus stearothermophilus ?
-
 ?
2.7.1.40 additional information allosteric enzyme: homotropic Geobacillus stearothermophilus ?
-
 ?
2.7.1.40 UDP + phosphoenolpyruvate less effective than ADP Geobacillus stearothermophilus UTP + pyruvate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.40 tetramer 4 * 62000-64000, SDS-PAGE Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.40 30
-
 assay at Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.40 25
-
 in 25 mM Tris-HCl buffer, pH 7.5, 50 mM KCl, 5 mM MgCl2, 50% ethylene glycol, inactivation within a few days Geobacillus stearothermophilus
2.7.1.40 65
-
 above, rapid inactivation, in the presence of 10 mM MgCl2: t1/2: more than 30 min, 50 mM KCl protects slightly, not phosphoenolpyruvate Geobacillus stearothermophilus
2.7.1.40 70
-
 5 min, inactivation, t1/2: 1 min Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.40 6.8
-
 60°C Geobacillus stearothermophilus
2.7.1.40 7.2
-
 30°C Geobacillus stearothermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.40 5.5 7.4 60°C, about half-maximal activity at pH 5.5 and pH 7.4 Geobacillus stearothermophilus
2.7.1.40 6.4 8.2 30°C, about half-maximal activity at pH 6.4 and pH 8.2 Geobacillus stearothermophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.40 5
-
 below, inactivation within a few days, 4°C Geobacillus stearothermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.40 AMP allosteric effector Geobacillus stearothermophilus
2.7.1.40 CMP activation Geobacillus stearothermophilus
2.7.1.40 CMP allosteric Geobacillus stearothermophilus
2.7.1.40 GDP activation Geobacillus stearothermophilus
2.7.1.40 GDP allosteric Geobacillus stearothermophilus
2.7.1.40 GMP activation Geobacillus stearothermophilus
2.7.1.40 GMP allosteric Geobacillus stearothermophilus