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Literature summary extracted from

  • Flynn, I.W.; Bowman, I.B.R.
    Purification and characterization of pyruvate kinase from Trypanosoma brucei (1980), Arch. Biochem. Biophys., 200, 401-409.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.40 cysteine activation, can replace dithiothreitol Trypanosoma brucei
2.7.1.40 D-fructose 1,6-diphosphate activation Trypanosoma brucei
2.7.1.40 D-fructose 1,6-diphosphate kinetics Trypanosoma brucei
2.7.1.40 D-glucose 6-phosphate
-
 Trypanosoma brucei
2.7.1.40 dithiothreitol activation Trypanosoma brucei
2.7.1.40 GSH activation, can replace dithiothreittol Trypanosoma brucei

General Stability

EC Number General Stability Organism
2.7.1.40 glycerol stabilizes during purification Trypanosoma brucei
2.7.1.40 unstable in aqueous solution, thiol protecting agents and glycerol stabilize Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.40 K+ above 100 mM, activates below Trypanosoma brucei
2.7.1.40 Na+ above 100 mM, activates below Trypanosoma brucei
2.7.1.40 NH4+ above 100 mM, activates below Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.40 0.082 0.4 ADP pH 7.4, 25°C Trypanosoma brucei
2.7.1.40 0.098 0.1 GDP pH 7.4, 25°C Trypanosoma brucei
2.7.1.40 0.41
-
 UDP pH 7.4, 25°C Trypanosoma brucei
2.7.1.40 2.53
-
 CDP pH 7.4, 25°C Trypanosoma brucei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.40 K+ requirement Trypanosoma brucei
2.7.1.40 K+ inhibits above 100 mM Trypanosoma brucei
2.7.1.40 Mg2+ requirement Trypanosoma brucei
2.7.1.40 Mn2+ requirement Trypanosoma brucei
2.7.1.40 monovalent cation requirement Trypanosoma brucei
2.7.1.40 Na+ inhibits above 100 mM Trypanosoma brucei
2.7.1.40 NH4+ requirement Trypanosoma brucei
2.7.1.40 NH4+ can replace K+ Trypanosoma brucei
2.7.1.40 NH4+ inhibits above 100 mM Trypanosoma brucei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.40 270000
-
 gel filtration Trypanosoma brucei

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Trypanosoma brucei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.40 partial Trypanosoma brucei

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.40 cell culture
-
 Trypanosoma brucei
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.40 additional information
-
-
 Trypanosoma brucei

Storage Stability

EC Number Storage Stability Organism
2.7.1.40 4°C, lyophilized lyzed trypanosomes, 3 h after resuspension, 20% loss of activity, storage in aqueous solution leads to a decrease in activity and cooperative nature of phosphoenolpyruvate-enzyme interaction Trypanosoma brucei
2.7.1.40 glycerol, 25% w/v, or 0.1 mM dithiothreitol stabilizes, more effective under refrigerated conditions than at room temperature, KCl, MgSO4, EDTA, phosphoenolpyruvate, ADP or (NH4)2SO4 decreases stability at either 4°C or 17°C Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate best nucleoside diphosphate substrate Trypanosoma brucei ATP + pyruvate
-
 ?
2.7.1.40 ADP + phosphoenolpyruvate positive cooperativity for phosphoenolpyruvate Trypanosoma brucei ATP + pyruvate
-
 ?
2.7.1.40 CDP + phosphoenolpyruvate less effective than ADP Trypanosoma brucei CTP + pyruvate
-
 ?
2.7.1.40 GDP + phosphoenolpyruvate less effective than ADP Trypanosoma brucei GTP + pyruvate
-
 ?
2.7.1.40 additional information preferred: purine nucleotides Trypanosoma brucei ?
-
 ?
2.7.1.40 UDP + phosphoenolpyruvate less effective than ADP Trypanosoma brucei UTP + pyruvate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.40 25
-
 assay at Trypanosoma brucei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.40 6.5 7
-
 Trypanosoma brucei