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Literature summary extracted from

  • Lin, M.; Turpin, D.H.; Plaxton, W.C.
    Pyruvate kinase isozymes from the green alga, Selenastrum minutum. II. Kinetic and regulatory properties (1989), Arch. Biochem. Biophys., 269, 228-238.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.40 asparagine activation Monoraphidium minutum
2.7.1.40 asparagine kinetics Monoraphidium minutum
2.7.1.40 D-glucose 1-phosphate slight activation, not isozyme PKp Monoraphidium minutum
2.7.1.40 D-glucose 6-phosphate activation Monoraphidium minutum
2.7.1.40 D-glucose 6-phosphate not: isozyme PKp Monoraphidium minutum
2.7.1.40 dihydroxyacetone phosphate activation Monoraphidium minutum
2.7.1.40 dihydroxyacetone phosphate kinetics Monoraphidium minutum
2.7.1.40 isoleucine slight activation, kinetics Monoraphidium minutum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.40 2,3-diphosphoglycerate
-
 Monoraphidium minutum
2.7.1.40 2-phosphoglycerate only isozyme PKp, not PKc Monoraphidium minutum
2.7.1.40 3-phosphoglycerate
-
 Monoraphidium minutum
2.7.1.40 aspartate only isozyme PKp, not PKc Monoraphidium minutum
2.7.1.40 ATP MgATP2- Monoraphidium minutum
2.7.1.40 citrate
-
 Monoraphidium minutum
2.7.1.40 D-ribose 5-phosphate only isozyme PKp, not PKc Monoraphidium minutum
2.7.1.40 D-ribulose 1,5-bisphosphate isozyme PKp, not PKc Monoraphidium minutum
2.7.1.40 fructose 1,6-diphosphate
-
 Monoraphidium minutum
2.7.1.40 glucose 6-phosphate only isozyme PKp, not PKc Monoraphidium minutum
2.7.1.40 isocitrate only isozyme PKp, not PKc Monoraphidium minutum
2.7.1.40 malate only isozyme PKp, not PKc Monoraphidium minutum
2.7.1.40 MgATP2-
-
 Monoraphidium minutum
2.7.1.40 oxalate
-
 Monoraphidium minutum
2.7.1.40 phosphate
-
 Monoraphidium minutum
2.7.1.40 Phosphoglycolate only isozyme PKp, not PKc Monoraphidium minutum
2.7.1.40 tyrosine
-
 Monoraphidium minutum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.40 0.03 0.05 ADP PKc-isozyme Monoraphidium minutum
2.7.1.40 0.049 0.1 IDP cosubstrate phosphoenolpyruvate Monoraphidium minutum
2.7.1.40 0.049 0.1 IDP isozyme PKc Monoraphidium minutum
2.7.1.40 0.18
-
 phosphoenolpyruvate isozyme PKp Monoraphidium minutum
2.7.1.40 0.2 0.35 ADP PKp-isozyme Monoraphidium minutum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.40 chloroplast 2 isozymes: a cytosolic and a chloroplastid one Monoraphidium minutum 9507
-
2.7.1.40 cytosol 2 isozymes: a cytosolic and a plastid one Monoraphidium minutum 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.40 divalent cation requirement Monoraphidium minutum
2.7.1.40 divalent cation requires both a divalent and a monovalent cation Monoraphidium minutum
2.7.1.40 K+
-
 Monoraphidium minutum
2.7.1.40 Mg2+
-
 Monoraphidium minutum
2.7.1.40 Mn2+ requirement Monoraphidium minutum
2.7.1.40 Mn2+ can partially replace Mg2+ Monoraphidium minutum
2.7.1.40 monovalent cation requirement Monoraphidium minutum
2.7.1.40 monovalent cation requires both a divalent and a monovalent cation Monoraphidium minutum
2.7.1.40 NH4+ requirement Monoraphidium minutum
2.7.1.40 NH4+ can replace K+ Monoraphidium minutum

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Monoraphidium minutum
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.40 cell culture
-
 Monoraphidium minutum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate best nucleoside diphosphate substrate Monoraphidium minutum ATP + pyruvate
-
 ?
2.7.1.40 CDP + phosphoenolpyruvate less effective than ADP Monoraphidium minutum CTP + pyruvate
-
 ?
2.7.1.40 CDP + phosphoenolpyruvate reaction with PKp-isozyme at 30%, with PKc-isozyme at 12% the rate of ADP Monoraphidium minutum CTP + pyruvate
-
 ?
2.7.1.40 GDP + phosphoenolpyruvate less effective than ADP Monoraphidium minutum GTP + pyruvate
-
 ?
2.7.1.40 GDP + phosphoenolpyruvate reaction for PKc-isozyme at 71%, for PKp-isozyme at 39% the rate of ADP Monoraphidium minutum GTP + pyruvate
-
 ?
2.7.1.40 IDP + phosphoenolpyruvate less effective than ADP Monoraphidium minutum ITP + pyruvate
-
 ?
2.7.1.40 IDP + phosphoenolpyruvate reaction with PKp-isozyme at 20%, with PKc-isozyme at 89% the rate of ADP Monoraphidium minutum ITP + pyruvate
-
 ?
2.7.1.40 UDP + phosphoenolpyruvate reaction at about 70% the rate of ADP, PKc-isozyme Monoraphidium minutum UTP + pyruvate
-
 ?
2.7.1.40 UDP + phosphoenolpyruvate less effective than ADP Monoraphidium minutum UTP + pyruvate
-
 ?
2.7.1.40 UDP + phosphoenolpyruvate reaction at about 31% the rate of ADP, PKp-isozyme Monoraphidium minutum UTP + pyruvate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.40 25
-
 assay at Monoraphidium minutum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.40 6.5
-
 isozyme PKp Monoraphidium minutum
2.7.1.40 7
-
 isozyme PKc Monoraphidium minutum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.40 5.2 8 isozyme PKp, about half-maximal activity at pH 5.2 and pH 8 Monoraphidium minutum
2.7.1.40 5.9 8.2 isozyme PKc, about half-maximal activity at pH 5.9 and pH 8.2 Monoraphidium minutum