BRENDA - Enzyme Database

Pyruvate kinase isozymes from the green alga, Selenastrum minutum. II. Kinetic and regulatory properties

Lin, M.; Turpin, D.H.; Plaxton, W.C.; Arch. Biochem. Biophys. 269, 228-238 (1989)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.40
asparagine
activation; kinetics
Monoraphidium minutum
2.7.1.40
D-glucose 1-phosphate
slight activation, not isozyme PKp
Monoraphidium minutum
2.7.1.40
D-glucose 6-phosphate
activation; not: isozyme PKp
Monoraphidium minutum
2.7.1.40
dihydroxyacetone phosphate
activation; kinetics
Monoraphidium minutum
2.7.1.40
isoleucine
slight activation, kinetics
Monoraphidium minutum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.40
2,3-diphosphoglycerate
-
Monoraphidium minutum
2.7.1.40
2-phosphoglycerate
only isozyme PKp, not PKc
Monoraphidium minutum
2.7.1.40
3-phosphoglycerate
-
Monoraphidium minutum
2.7.1.40
aspartate
only isozyme PKp, not PKc
Monoraphidium minutum
2.7.1.40
ATP
MgATP2-
Monoraphidium minutum
2.7.1.40
citrate
-
Monoraphidium minutum
2.7.1.40
D-ribose 5-phosphate
only isozyme PKp, not PKc
Monoraphidium minutum
2.7.1.40
D-ribulose 1,5-bisphosphate
isozyme PKp, not PKc
Monoraphidium minutum
2.7.1.40
fructose 1,6-diphosphate
-
Monoraphidium minutum
2.7.1.40
glucose 6-phosphate
only isozyme PKp, not PKc
Monoraphidium minutum
2.7.1.40
isocitrate
only isozyme PKp, not PKc
Monoraphidium minutum
2.7.1.40
malate
only isozyme PKp, not PKc
Monoraphidium minutum
2.7.1.40
MgATP2-
-
Monoraphidium minutum
2.7.1.40
oxalate
-
Monoraphidium minutum
2.7.1.40
phosphate
-
Monoraphidium minutum
2.7.1.40
Phosphoglycolate
only isozyme PKp, not PKc
Monoraphidium minutum
2.7.1.40
tyrosine
-
Monoraphidium minutum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.40
0.03
0.05
ADP
PKc-isozyme
Monoraphidium minutum
2.7.1.40
0.049
0.1
IDP
cosubstrate phosphoenolpyruvate; isozyme PKc
Monoraphidium minutum
2.7.1.40
0.18
-
phosphoenolpyruvate
isozyme PKp
Monoraphidium minutum
2.7.1.40
0.2
0.35
ADP
PKp-isozyme
Monoraphidium minutum
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.40
chloroplast
2 isozymes: a cytosolic and a chloroplastid one
Monoraphidium minutum
9507
-
2.7.1.40
cytosol
2 isozymes: a cytosolic and a plastid one
Monoraphidium minutum
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.40
divalent cation
requirement; requires both a divalent and a monovalent cation
Monoraphidium minutum
2.7.1.40
K+
-
Monoraphidium minutum
2.7.1.40
Mg2+
-
Monoraphidium minutum
2.7.1.40
Mn2+
can partially replace Mg2+; requirement
Monoraphidium minutum
2.7.1.40
monovalent cation
requirement; requires both a divalent and a monovalent cation
Monoraphidium minutum
2.7.1.40
NH4+
can replace K+; requirement
Monoraphidium minutum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.40
Monoraphidium minutum
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.40
cell culture
-
Monoraphidium minutum
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.40
ADP + phosphoenolpyruvate
best nucleoside diphosphate substrate
641576
Monoraphidium minutum
ATP + pyruvate
-
-
-
-
2.7.1.40
CDP + phosphoenolpyruvate
less effective than ADP
641576
Monoraphidium minutum
CTP + pyruvate
-
-
-
?
2.7.1.40
CDP + phosphoenolpyruvate
reaction with PKp-isozyme at 30%, with PKc-isozyme at 12% the rate of ADP
641576
Monoraphidium minutum
CTP + pyruvate
-
-
-
?
2.7.1.40
GDP + phosphoenolpyruvate
less effective than ADP
641576
Monoraphidium minutum
GTP + pyruvate
-
-
-
?
2.7.1.40
GDP + phosphoenolpyruvate
reaction for PKc-isozyme at 71%, for PKp-isozyme at 39% the rate of ADP
641576
Monoraphidium minutum
GTP + pyruvate
-
-
-
?
2.7.1.40
IDP + phosphoenolpyruvate
less effective than ADP
641576
Monoraphidium minutum
ITP + pyruvate
-
-
-
?
2.7.1.40
IDP + phosphoenolpyruvate
reaction with PKp-isozyme at 20%, with PKc-isozyme at 89% the rate of ADP
641576
Monoraphidium minutum
ITP + pyruvate
-
-
-
?
2.7.1.40
UDP + phosphoenolpyruvate
reaction at about 70% the rate of ADP, PKc-isozyme
641576
Monoraphidium minutum
UTP + pyruvate
-
-
-
?
2.7.1.40
UDP + phosphoenolpyruvate
less effective than ADP
641576
Monoraphidium minutum
UTP + pyruvate
-
-
-
?
2.7.1.40
UDP + phosphoenolpyruvate
reaction at about 31% the rate of ADP, PKp-isozyme
641576
Monoraphidium minutum
UTP + pyruvate
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.40
25
-
assay at
Monoraphidium minutum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.40
6.5
-
isozyme PKp
Monoraphidium minutum
2.7.1.40
7
-
isozyme PKc
Monoraphidium minutum
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.40
5.2
8
isozyme PKp, about half-maximal activity at pH 5.2 and pH 8
Monoraphidium minutum
2.7.1.40
5.9
8.2
isozyme PKc, about half-maximal activity at pH 5.9 and pH 8.2
Monoraphidium minutum
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.40
asparagine
activation; kinetics
Monoraphidium minutum
2.7.1.40
D-glucose 1-phosphate
slight activation, not isozyme PKp
Monoraphidium minutum
2.7.1.40
D-glucose 6-phosphate
activation; not: isozyme PKp
Monoraphidium minutum
2.7.1.40
dihydroxyacetone phosphate
activation; kinetics
Monoraphidium minutum
2.7.1.40
isoleucine
slight activation, kinetics
Monoraphidium minutum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.40
2,3-diphosphoglycerate
-
Monoraphidium minutum
2.7.1.40
2-phosphoglycerate
only isozyme PKp, not PKc
Monoraphidium minutum
2.7.1.40
3-phosphoglycerate
-
Monoraphidium minutum
2.7.1.40
aspartate
only isozyme PKp, not PKc
Monoraphidium minutum
2.7.1.40
ATP
MgATP2-
Monoraphidium minutum
2.7.1.40
citrate
-
Monoraphidium minutum
2.7.1.40
D-ribose 5-phosphate
only isozyme PKp, not PKc
Monoraphidium minutum
2.7.1.40
D-ribulose 1,5-bisphosphate
isozyme PKp, not PKc
Monoraphidium minutum
2.7.1.40
fructose 1,6-diphosphate
-
Monoraphidium minutum
2.7.1.40
glucose 6-phosphate
only isozyme PKp, not PKc
Monoraphidium minutum
2.7.1.40
isocitrate
only isozyme PKp, not PKc
Monoraphidium minutum
2.7.1.40
malate
only isozyme PKp, not PKc
Monoraphidium minutum
2.7.1.40
MgATP2-
-
Monoraphidium minutum
2.7.1.40
oxalate
-
Monoraphidium minutum
2.7.1.40
phosphate
-
Monoraphidium minutum
2.7.1.40
Phosphoglycolate
only isozyme PKp, not PKc
Monoraphidium minutum
2.7.1.40
tyrosine
-
Monoraphidium minutum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.40
0.03
0.05
ADP
PKc-isozyme
Monoraphidium minutum
2.7.1.40
0.049
0.1
IDP
cosubstrate phosphoenolpyruvate; isozyme PKc
Monoraphidium minutum
2.7.1.40
0.18
-
phosphoenolpyruvate
isozyme PKp
Monoraphidium minutum
2.7.1.40
0.2
0.35
ADP
PKp-isozyme
Monoraphidium minutum
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.40
chloroplast
2 isozymes: a cytosolic and a chloroplastid one
Monoraphidium minutum
9507
-
2.7.1.40
cytosol
2 isozymes: a cytosolic and a plastid one
Monoraphidium minutum
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.40
divalent cation
requirement; requires both a divalent and a monovalent cation
Monoraphidium minutum
2.7.1.40
K+
-
Monoraphidium minutum
2.7.1.40
Mg2+
-
Monoraphidium minutum
2.7.1.40
Mn2+
can partially replace Mg2+; requirement
Monoraphidium minutum
2.7.1.40
monovalent cation
requirement; requires both a divalent and a monovalent cation
Monoraphidium minutum
2.7.1.40
NH4+
can replace K+; requirement
Monoraphidium minutum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.40
cell culture
-
Monoraphidium minutum
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.40
ADP + phosphoenolpyruvate
best nucleoside diphosphate substrate
641576
Monoraphidium minutum
ATP + pyruvate
-
-
-
-
2.7.1.40
CDP + phosphoenolpyruvate
less effective than ADP
641576
Monoraphidium minutum
CTP + pyruvate
-
-
-
?
2.7.1.40
CDP + phosphoenolpyruvate
reaction with PKp-isozyme at 30%, with PKc-isozyme at 12% the rate of ADP
641576
Monoraphidium minutum
CTP + pyruvate
-
-
-
?
2.7.1.40
GDP + phosphoenolpyruvate
less effective than ADP
641576
Monoraphidium minutum
GTP + pyruvate
-
-
-
?
2.7.1.40
GDP + phosphoenolpyruvate
reaction for PKc-isozyme at 71%, for PKp-isozyme at 39% the rate of ADP
641576
Monoraphidium minutum
GTP + pyruvate
-
-
-
?
2.7.1.40
IDP + phosphoenolpyruvate
less effective than ADP
641576
Monoraphidium minutum
ITP + pyruvate
-
-
-
?
2.7.1.40
IDP + phosphoenolpyruvate
reaction with PKp-isozyme at 20%, with PKc-isozyme at 89% the rate of ADP
641576
Monoraphidium minutum
ITP + pyruvate
-
-
-
?
2.7.1.40
UDP + phosphoenolpyruvate
reaction at about 70% the rate of ADP, PKc-isozyme
641576
Monoraphidium minutum
UTP + pyruvate
-
-
-
?
2.7.1.40
UDP + phosphoenolpyruvate
less effective than ADP
641576
Monoraphidium minutum
UTP + pyruvate
-
-
-
?
2.7.1.40
UDP + phosphoenolpyruvate
reaction at about 31% the rate of ADP, PKp-isozyme
641576
Monoraphidium minutum
UTP + pyruvate
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.40
25
-
assay at
Monoraphidium minutum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.40
6.5
-
isozyme PKp
Monoraphidium minutum
2.7.1.40
7
-
isozyme PKc
Monoraphidium minutum
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.40
5.2
8
isozyme PKp, about half-maximal activity at pH 5.2 and pH 8
Monoraphidium minutum
2.7.1.40
5.9
8.2
isozyme PKc, about half-maximal activity at pH 5.9 and pH 8.2
Monoraphidium minutum